| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-07047SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-07049SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-93829-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-95194-0.02ml | |||
![Western Blot: PDK4 Antibody [NBP1-07047] - Western Blot on human heart protein lysate.](http://images.novusbio.com/fullsize/PDK4-Antibody-Western-Blot-NBP1-07047-img0002.jpg "Western Blot: PDK4 Antibody [NBP1-07047] - Western Blot on human heart protein lysate.")
![Western Blot: PDK4 Antibody [NBP1-07047] - Mouse liver. NuPAGE 4-12%. This image was submitted via customer review.](http://images.novusbio.com/fullsize/PDK4-Antibody-Western-Blot-NBP1-07047-img0003.jpg)
![Western Blot: PDK4 Antibody [NBP1-07047] - The protein expression levels in QF muscle. Representative Western blot images. n = 14-15 animals/group. * = vs. LFsed (P 0.1). Black bars = sedentary, grey bars = running. Image collected and cropped by CiteAb from the following publication (//nutritionandmetabolism.biomedcentral.com/articles/10.1186/1743-7075-9-53), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PDK4-Antibody-Western-Blot-NBP1-07047-img0004.jpg)
![Western Blot: PDK4 Antibody - BSA Free [NBP1-07047] - Genetic manipulations can alter PDH E1 alpha phosphorylation in response to hypoxia.(a) MIA PaCa-2 control (Hg), silenced HIF1 alpha (shHIF1 alpha ) or silenced PDHK1 (shPDHK1) & PDP1 overexpressing cells were incubated for 16 h in normoxia or hypoxia (0.5% O2) & phospho-serine E1 alpha detected by Western blot showing that all three modifications can have an inhibitory effect on hypoxic Ser232 phosphorylation. (b) Increased pSer232-E1 alpha detection after E1 alpha immunoprecipitation from lysates of cells treated as in (a). (c) The same analysis as in (a) after treatment with 16 h 1 mM DMOG. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27498883), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-07047_rabbit-polyclonal-pdk4-antibody-31020241534333.jpg)
![Western Blot: PDK4 Antibody - BSA Free [NBP1-07047] - Hypoxia inhibits mitochondrial OCR & PDH activity & induces PDHK1 protein & activity.(a) Ratio of oxygen consumption rate (OCR) to extracellular acidification rate (ECAR) measured by Seahorse XF in MIA PaCa-2, PANC-1, & SU.86.86 cell lines in high (25 mM) or low (0.5 mM) glucose incubated overnight with or without 1 mM DMOG. (mean ± SEM, two-tailed Student’s t-test, **p < 0.01, ***p < 0.001) (b) Cell-based PDH activity assay in cells incubated 16 h in normoxia, hypoxia (0.5% O2) or 1 mM DMOG. (mean ± SD, one-way ANOVA, *p < 0.05, **p < 0.01, ***p < 0.001) (c) Western blots of HIF alpha isoforms, pyruvate dehydrogenase kinase isoforms (PDHKs), phosphatase (PDP1 – lower band *), target phosphorylated serine residues on E1 alpha & total E1 alpha after overnight incubation in normoxia or hypoxia (0.5% O2) at 25 or 5 mM glucose as indicated. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27498883), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-07047_rabbit-polyclonal-pdk4-antibody-310202416171443.jpg)
![Western Blot: PDK4 Antibody [NBP1-07049] - Western Blot on human heart protein lysate.](http://images.novusbio.com/fullsize/PDK4-Antibody-Western-Blot-NBP1-07049-img0003.jpg "Western Blot: PDK4 Antibody [NBP1-07049] - Western Blot on human heart protein lysate.")
![Immunocytochemistry/Immunofluorescence: PDK4 Antibody [NBP1-07049] - PDK4 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/PDK4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-07049-img0004.jpg)
![Western Blot: PDK4 Antibody [NBP1-54723] - Titration: 0.2-1 ug/ml Positive Control: Transfected 293T.](http://images.novusbio.com/fullsize/PDK4-Antibody-Western-Blot-NBP1-54723-img0005.jpg "Western Blot: PDK4 Antibody [NBP1-54723] - Titration: 0.2-1 ug/ml Positive Control: Transfected 293T.")
![Immunohistochemistry-Paraffin: PDK4 Antibody [NBP1-54723] - Human Muscle Tissue, Skeletal muscle cells (Indicated with Arrows) 4-8ug/ml.](http://images.novusbio.com/fullsize/PDK4-Antibody-Immunohistochemistry-Paraffin-NBP1-54723-img0006.jpg)
![Western Blot: PDK4 Antibody [NBP1-54723] - Sample Type: Huh7 HepG2 (50ug) Primary Antibody Dilution: 1:500 Image Submitted By: Partha Kasturi University of Kansas Medical Center.](http://images.novusbio.com/fullsize/PDK4-Antibody-Western-Blot-NBP1-54723-img0004.jpg)
![Western Blot: PDK4 Antibody [NBP2-93829] - Analysis of various lysates, using PDK4 antibody at 1:2350 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/PDK4-Antibody-Western-Blot-NBP2-93829-img0007.jpg "Western Blot: PDK4 Antibody [NBP2-93829] - Analysis of various lysates, using PDK4 antibody at 1:2350 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: PDK4 Antibody [NBP2-93829] - Immunofluorescence analysis of U-2 OS cells using PDK4 antibody (NBP2-93829) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/PDK4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-93829-img0003.jpg)
![Immunohistochemistry-Paraffin: PDK4 Antibody [NBP2-93829] - Mouse heart using PDK4 Rabbit pAb (NBP2-93829) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PDK4-Antibody-Immunohistochemistry-Paraffin-NBP2-93829-img0006.jpg)
![Immunohistochemistry-Paraffin: PDK4 Antibody [NBP2-93829] - Rat heart using PDK4 Rabbit pAb (NBP2-93829) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PDK4-Antibody-Immunohistochemistry-Paraffin-NBP2-93829-img0004.jpg)
![Immunohistochemistry-Paraffin: PDK4 Antibody [NBP2-93829] - Human placenta using PDK4 Rabbit pAb (NBP2-93829) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PDK4-Antibody-Immunohistochemistry-Paraffin-NBP2-93829-img0005.jpg)
![Western Blot: PDK4 Antibody [NBP2-95194] - Western blot analysis of extracts of various cell lines, using PDK4 antibody (NBP2-95194) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/PDK4 Antibody-Western Blot-NBP2-95194-img0003.jpg "Western Blot: PDK4 Antibody [NBP2-95194] - Western blot analysis of extracts of various cell lines, using PDK4 antibody (NBP2-95194) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: PDK4 Antibody [NBP2-95194] - Immunohistochemistry of paraffin-embedded human placenta using PDK4 Rabbit pAb (NBP2-95194) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PDK4-Antibody-Immunohistochemistry-Paraffin-NBP2-95194-img0002.jpg)
![Immunohistochemistry-Paraffin: PDK4 Antibody [NBP2-95194] - Immunohistochemistry of paraffin-embedded rat heart using PDK4 Rabbit pAb (NBP2-95194) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PDK4-Antibody-Immunohistochemistry-Paraffin-NBP2-95194-img0001.jpg)
![Immunohistochemistry-Paraffin: PDK4 Antibody [NBP2-49235] - Staining of human heart muscle shows strong cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/PDK4-Antibody-Immunohistochemistry-Paraffin-NBP2-49235-img0002.jpg "Immunohistochemistry-Paraffin: PDK4 Antibody [NBP2-49235] - Staining of human heart muscle shows strong cytoplasmic positivity in myocytes.")
![ELISA: Human PDK4 ELISA Kit (Colorimetric) [NBP2-81231] - Samples were spiked with high concentrations of Human PDK4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-81231_human-pdk4-elisa-kit-colorimetric-1322024160120.jpg "ELISA: Human PDK4 ELISA Kit (Colorimetric) [NBP2-81231] - Samples were spiked with high concentrations of Human PDK4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human PDK4 ELISA Kit (Colorimetric) [NBP2-81231] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-81231_human-pdk4-elisa-kit-colorimetric-4320249141623.jpg)
![ELISA: Rat PDK4 ELISA Kit (Colorimetric) [NBP2-81233] - Samples were spiked with high concentrations of Rat PDK4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-81233_rat-pdk4-elisa-kit-colorimetric-132202416254069.jpg "ELISA: Rat PDK4 ELISA Kit (Colorimetric) [NBP2-81233] - Samples were spiked with high concentrations of Rat PDK4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat PDK4 ELISA Kit (Colorimetric) [NBP2-81233] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-81233_rat-pdk4-elisa-kit-colorimetric-43202495507.jpg)
![ELISA: Mouse PDK4 ELISA Kit (Colorimetric) [NBP2-81232] - Samples were spiked with high concentrations of Mouse PDK4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-81232_mouse-pdk4-elisa-kit-colorimetric-132202416235730.jpg "ELISA: Mouse PDK4 ELISA Kit (Colorimetric) [NBP2-81232] - Samples were spiked with high concentrations of Mouse PDK4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse PDK4 ELISA Kit (Colorimetric) [NBP2-81232] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-81232_mouse-pdk4-elisa-kit-colorimetric-432024941540.jpg)