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PDK2 Antibody (5F8)

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Western Blot: PDK2 Antibody (5F8) [H00005164-M02] - PDK2 monoclonal antibody (M02), clone 5F8. Analysis of PDK2 expression in A-431.
Western Blot: PDK2 Antibody (5F8) [H00005164-M02] - PDK2 monoclonal antibody (M02), clone 5F8. Analysis of PDK2 expression in A-431.
Immunohistochemistry-Paraffin: PDK2 Antibody (5F8) [H00005164-M02] - Analysis of monoclonal antibody to PDK2 on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 2 ug/ml.
Western Blot: PDK2 Antibody (5F8) [H00005164-M02] - PDK2 monoclonal antibody (M02), clone 5F8 Analysis of PDK2 expression in HeLa.
Western Blot: PDK2 Antibody (5F8) [H00005164-M02] - PDK2 monoclonal antibody (M02), clone 5F8. Analysis of PDK2 expression in 293.
ELISA: PDK2 Antibody (5F8) [H00005164-M02] - Detection limit for recombinant GST tagged PDK2 is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Clone
5F8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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PDK2 Antibody (5F8) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
PDK2 (AAH05811, 187 a.a. ~ 276 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. HIGSIDPNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEINAANSKQPIHMVYVPSHLYHMLFELFKNAMRATVESHESSLILPPIKVM
Specificity
PDK2 (5F8)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PDK2
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 2 ug/ml
  • Western Blot 1:100-1:2000
Application Notes
WB usage on cell lysate and recombinant protein reported in scientific literature (PMID: 22123926).
Control
HeLa Whole Cell Lysate
Reviewed Applications
Read 1 Review rated 3
using
H00005164-M02 in the following applications:
Publications
Read Publication using H00005164-M02.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PDK2 Antibody (5F8)

  • EC 2.7.11
  • EC 2.7.11.2
  • lipoamide, kinase isozyme 2, mitochondrial
  • PDH kinase 2
  • PDHK2
  • PDK2
  • PDKII
  • Pyruvate dehydrogenase kinase isoform 2
  • pyruvate dehydrogenase kinase, isoenzyme 2
  • pyruvate dehydrogenase kinase, isozyme 2

Background

PDK2 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PDK2 Antibody (H00005164-M02)(1)


Review for PDK2 Antibody (H00005164-M02) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using H00005164-M02:
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WB
(1)
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Images Ratings Applications Species Date Details
  3
reviewed by:
Verified Customer
 WB Human 08/22/2015
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Summary

ApplicationWestern Blot
Sample TestedHuman cancer cell whole cell lysate
SpeciesHuman

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for PDK2 Antibody (H00005164-M02) (0)

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Control Lysate(s)

HeLa Whole Cell Lysate

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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 Verified Customer
08/22/2015
Application: WB
Species: Human
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Bioinformatics

Gene Symbol PDK2
Entrez
OMIM
Uniprot