Recombinant Human PDGF-D/SCDGFB His Protein Summary
Description |
A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 250-370 of Human PDGF-D/SCDGFB Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMSYHDR KSKVDLDRLN DDAKRYSCTP RNYSVNIREE LKLANVVFFP RCLLVQRCGG NCGCGTVNWR SCTCNSGKTV KKYHEVLQFE PGHIKRRGRA KTMALVDIQL DHHERCDCIC SSRPPR |
Source |
E. coli |
Protein/Peptide Type |
Recombinant Protein |
Gene |
PDGFD |
Purity |
>90%, by SDS-PAGE |
Applications/Dilutions
Dilutions |
|
Application Notes |
Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays. |
Theoretical MW |
16.6 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol |
Preservative |
No Preservative |
Concentration |
1 mg/ml |
Purity |
>90%, by SDS-PAGE |
Alternate Names for Recombinant Human PDGF-D/SCDGFB His Protein
Background
Platelet-derived growth factor D, also known as PDGFD, plays an important role in wound healing. This protein induces macrophage recruitment, increased interstitial pressure, and blood vessel maturation during angiogenesis. This protein can initiate events that lead to a mesangial proliferative glomerulonephritis, including influx of monocytes and macrophages and production of extracellular matrix. Recombinant human PDGFD protein, fused to His-tag at N-terminus, was expressed in E.coli.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 3 months from date of receipt.
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