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PARP2 Antibody

Images

 
Western Blot: PARP2 Antibody [NB100-2556] - (1ug/ml) staining of Human Spleen Lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: PARP2 Antibody [NB100-2556] - (5ug/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: PARP2 Antibody [NB100-2556] - (5ug/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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PARP2 Antibody Summary

Immunogen
Peptide with sequence C-LDLFEVEKDGEKE corresponding to internal region according to NP_005475.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PARP2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 1 - 3 ug/ml
Application Notes
WB: Approx. 70 kDa band observed in human spleen lysates (calculated MW of 60.8 kDa band according to NP_005475.1).
IHC: Paraffin embedded Human Skin and Adrenal Gland.
Control
Human Spleen Whole Tissue Lysate (Adult Whole Normal)
Publications
Read Publication using NB100-2556.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PARP2 Antibody

  • ADP-ribosyltransferase (NAD+; poly(ADP-ribose) polymerase)-like 2
  • ADPRT2
  • ADPRT-2
  • ADPRT2pADPRT-2
  • ADPRTL2
  • ADPRTL2EC 2.4.2.30
  • ADPRTL3
  • ARTD2
  • EC 2.4.2.30
  • hPARP-2
  • NAD(+) ADP-ribosyltransferase 2
  • pADPRT-2
  • PARP2
  • PARP-2
  • poly (ADP-ribose) polymerase 2
  • poly (ADP-ribose) polymerase family, member 2
  • poly (ADP-ribosyl) transferase-like 2
  • poly [ADP-ribose] polymerase 2
  • poly(ADP-ribose) synthetase
  • Poly[ADP-ribose] synthase 2
  • poly[ADP-ribose] synthetase 2

Background

This gene encodes poly(ADP-ribosyl)transferase-like 2 protein, which contains a catalytic domain and is capable of catalyzing a poly(ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. The basic residues within the N-terminal region of this protein may bear potential DNA-binding properties, and may be involved in the nuclear and/or nucleolar targeting of the protein. Two alternatively spliced transcript variants encoding distinct isoforms have been found.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PARP2 Antibody (NB100-2556)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for PARP2 Antibody (NB100-2556) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional PARP2 Products

Array NB100-2556

Research Areas for PARP2 Antibody (NB100-2556)

Find related products by research area.

Blogs on PARP2.

PARP Antibody Assays aid both Apoptosis and Cancer Research
The PARP (Poly(ADP-ribose) polymerase) protein is a zinc-dependant nuclear enzyme whose main role is to detect and repair DNA single-strand breaks (SSB). However, PARP antibody research has revealed there are at least 17 PARP proteins, which also play...  Read full blog post.

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Bioinformatics

Gene Symbol PARP2
Entrez
Uniprot