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PARD3/Par3 Antibody (833517)

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PARD3/Par3 was detected in immersion fixed HEK293 human embryonic kidney cell line using Mouse Anti-Human PARD3/Par3 Monoclonal Antibody (Catalog # MAB8030) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
PARD3/Par3 was detected in immersion fixed HEK293 human embryonic kidney cell line using Mouse Anti-Human PARD3/Par3 Monoclonal Antibody (Catalog # MAB8030) at 25 µg/mL for 3 hours at room temperature. Cells were ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF
Clone
833517
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB8030-SP
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PARD3/Par3 Antibody (833517) Summary

Immunogen
E. coli-derived recombinant human PARD3/Par3
Tyr451-Glu555
Accession # Q8TEW0
Specificity
Detects human PARD3/Par3 in ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human PARD3b/Par3b is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PARD3
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
Publications
Read Publications using
MAB8030 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PARD3/Par3 Antibody (833517)

  • ASIP
  • Baz
  • Bazooka
  • FLJ21015
  • par-3 partitioning defective 3 homolog (C. elegans)
  • PAR3
  • PAR3A
  • PAR3C.elegans) homolog
  • PARD3
  • SE2-5L16
  • SE2-5LT1
  • SE2-5T2

Background

PARD3 (partitioning defective 3 homolog), also called Par3, is a widely expressed adapter protein involved in asymmetrical cell division, cell polarization, and tight junction formation. It forms a cell polarity complex with Par6 and aPKC (atypical protein kinase C), linking to PTEN (phosphatase and tensin homolog) in polarized epithelia and VE-Cadherin in polarized endothelia. The region used as an immunogen, human PARD3 amino acids (aa) 451-555 (of 1356 aa in full-length PARD3) shares 97% and 96% aa sequence identity with mouse and rat PARD3, respectively. It includes the second of three PDZ domains, a region which is involved in heterophilic interactions and is present within all 10 reported PARD3 isoforms that vary in length from 988 aa to 1356 aa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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PARD3/Par3
Antibody (833517)
MAB8030
PARD3/Par3 Antibody (833517)
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Publications for PARD3/Par3 Antibody (MAB8030)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC, IHC, Western Blot.


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(1)
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Bioinformatics

Gene Symbol PARD3
Uniprot