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Rat PAR2 ELISA Kit (Colorimetric)

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ELISA: Rat PAR2 ELISA Kit (Colorimetric) [NBP3-06968] - Samples were spiked with high concentrations of Rat PAR2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Rat PAR2 ELISA Kit (Colorimetric) [NBP3-06968] - Samples were spiked with high concentrations of Rat PAR2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of ...read more
ELISA: Rat PAR2 ELISA Kit (Colorimetric) [NBP3-06968] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, Plasma, Cell supernatant
Standard Curve Range
7.81 - 500 nIU/mL
Sensitivity
4.69 nIU/mL

Order Details

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Rat PAR2 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat PAR2 in samples. No significant cross-reactivity or interference between Rat PAR2 and analogues was observed.
Standard Curve Range
7.81 - 500 nIU/mL
Sensitivity
4.69 nIU/mL
Assay Type
Sandwich-ELISA
Inter-Assay
%CV > 5.47
Intra-Assay
%CV > 5.55
Spike Recovery
87-107%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
F2RL1

Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Rat PAR2 ELISA Kit (Colorimetric)

  • CFIIRL1
  • coagulation factor II (thrombin) receptor-like 1
  • Coagulation factor II receptor-like 1
  • F2RL1
  • GPCR11
  • GPR11protease-activated receptor 2
  • G-protein coupled receptor 11
  • PAR2
  • PAR-2
  • PAR2G protein-coupled receptor-11
  • proteinase-activated receptor 2
  • proteinase-activated receptor-2
  • Thrombin receptor-like 1

Background

The Proteinase-activated receptor 2 (PAR2) is a member of the proteinase-activated receptor subfamily. It is activated through proteolytic exposure of an occult tethered ligand by trypsin and trypsin-like proteases. This is in contrast to other members of the subfamily which are activated by the protease thrombin. PAR2 has been implicated in acute inflammatory response, asthma, and pain transmission. PAR2 expression has been documented in the periphery. ESTs have been isolated from adrenal, brain, breast, heart/melanocyte/uterus, kidney, lung, and vessel libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Rat PAR2 ELISA Kit
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Rat PAR2 ELISA Kit (Colorimetric)
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Applications: ELISA

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Product PDFs

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  Rat PAR2 ELISA Kit (Colorimetric) Manual
  PDF Datasheet
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Bioinformatics

Gene Symbol F2RL1