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![Western Blot: PAR2 Antibody (9X8E6) [NBP3-16555] - Analysis of extracts of various cell lines, using PAR2 Rabbit mAb (NBP3-16555) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/PAR2-Antibody-9X8E6-Western-Blot-NBP3-16555-img0002.jpg "Western Blot: PAR2 Antibody (9X8E6) [NBP3-16555] - Analysis of extracts of various cell lines, using PAR2 Rabbit mAb (NBP3-16555) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Western Blot: PAR2 Antibody (9X8E6) [NBP3-16555] - Analysis of extracts of Rat large intestine, using PAR2 Rabbit mAb (NBP3-16555) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/PAR2-Antibody-9X8E6-Western-Blot-NBP3-16555-img0003.jpg)
![Immunohistochemistry: PAR2 Antibody (9X8E6) [NBP3-16555] - Immunohistochemistry analysis of paraffin-embedded Human gastric cancer tissue using PAR2 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16555_rabbit-par2-mab-9x8e6-52202511365142.jpg)
![Immunohistochemistry: PAR2 Antibody (9X8E6) [NBP3-16555] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using PAR2 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16555_rabbit-par2-mab-9x8e6-5220251265325.jpg)
![Immunohistochemistry: PAR2 Antibody (9X8E6) [NBP3-16555] - Immunohistochemistry analysis of paraffin-embedded Human breast tissue using PAR2 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16555_rabbit-par2-mab-9x8e6-52202511361293.jpg)
![Immunohistochemistry: PAR2 Antibody (9X8E6) [NBP3-16555] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using PAR2 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16555_rabbit-par2-mab-9x8e6-5220251138377.jpg)
![Immunohistochemistry: PAR2 Antibody (9X8E6) [PAR2] - Immunohistochemistry analysis of paraffin-embedded Human colon tissue using PAR2 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16555_rabbit-par2-mab-9x8e6-52202517213570.jpg)
![Immunohistochemistry: PAR2 Antibody (9X8E6) [PAR2] - Immunohistochemistry analysis of paraffin-embedded Human breast tissue using PAR2 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16555_rabbit-par2-mab-9x8e6-52202517273587.jpg)
![Immunohistochemistry: PAR2 Antibody (9X8E6) [PAR2] - Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using PAR2 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16555_rabbit-par2-mab-9x8e6-5220251727358.jpg)
![Immunohistochemistry: PAR2 Antibody (9X8E6) [PAR2] - Immunohistochemistry analysis of paraffin-embedded Human pancreatic cancer tissue using PAR2 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16555_rabbit-par2-mab-9x8e6-52202517213531.jpg)
![Immunohistochemistry: PAR2 Antibody (9X8E6) [PAR2] - Immunohistochemistry analysis of paraffin-embedded Human gastric cancer tissue using PAR2 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16555_rabbit-par2-mab-9x8e6-52202517192960.jpg)
![Western Blot: PAR2 Antibody [NBP2-11250] - 1:500 antibody dilution in DiluObuffer. Apparent MW is 50 KDa.](http://images.novusbio.com/fullsize/PAR2-Antibody-Western-Blot-NBP2-11250-img0003.jpg "Western Blot: PAR2 Antibody [NBP2-11250] - 1:500 antibody dilution in DiluObuffer. Apparent MW is 50 KDa.")
![Immunohistochemistry-Paraffin: PAR2 Antibody [NBP2-11250] - 1:100 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification.](http://images.novusbio.com/fullsize/PAR2-Antibody-Immunohistochemistry-Paraffin-NBP2-11250-img0004.jpg)
![Immunohistochemistry-Paraffin: PAR2 Antibody [NBP2-11250] - 1:100 dilution in IHC blocking buffer. DAB (brown staining and Hematoxlyn QS (blue) counsterstain. FFPE section](http://images.novusbio.com/fullsize/PAR2-Antibody-Immunohistochemistry-Paraffin-NBP2-11250-img0002.jpg)
![Immunohistochemistry-Paraffin: PAR2 Antibody [NLS252] - Anti-F2RL1 / PAR2 antibody IHC of human Lung, Adenocarcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/PAR2-Antibody-Immunohistochemistry-Paraffin-NLS252-img0005.jpg "Immunohistochemistry-Paraffin: PAR2 Antibody [NLS252] - Anti-F2RL1 / PAR2 antibody IHC of human Lung, Adenocarcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: PAR2 Antibody [NLS252] - Analysis of anti-F2RL1 / PAR2 antibody with human kidney.](http://images.novusbio.com/fullsize/PAR2-Antibody-Immunohistochemistry-Paraffin-NLS252-img0001.jpg)
![Immunohistochemistry-Paraffin: PAR2 Antibody [NLS252] - Analysis of anti-F2RL1 / PAR2 antibody with human adrenal.](http://images.novusbio.com/fullsize/PAR2-Antibody-Immunohistochemistry-Paraffin-NLS252-img0003.jpg)
![Immunohistochemistry-Paraffin: PAR2 Antibody [NLS252] - Anti-F2RL1 / PAR2 antibody IHC of human Breast, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/PAR2-Antibody-Immunohistochemistry-Paraffin-NLS252-img0004.jpg)
![Immunohistochemistry-Paraffin: PAR2 Antibody [NLS252] - Anti-F2RL1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/PAR2-Antibody-Immunohistochemistry-Paraffin-NLS252-img0006.jpg)
![Western Blot: PAR2 Antibody (SR1993) [NBP3-22142] - Western blot analysis using NBP3-22142 in K562 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22142_rabbit-par2-mab-sr1993-57202315213814.jpg "Western Blot: PAR2 Antibody (SR1993) [NBP3-22142] - Western blot analysis using NBP3-22142 in K562 cell lysate.")
![Western Blot: PAR2 Antibody (JE35-05) [NBP3-32715] - Western blot analysis of PAR2 on different lysates with Rabbit anti-PAR2 antibody (NBP3-32715) at 1/1,000 dilution. Lane 1: HCT 116 cell lysate (15 ug/Lane) Lane 2: HepG2 cell lysate (15 ug/Lane) Lane 3: PANC-1 cell lysate (15 ug/Lane) Lane 4: PC-3M cell lysate (15 ug/Lane) Lane 5: AGS cell lysate (15 ug/Lane) Lane 6: SGC-7901 cell lysate (15 ug/Lane) Lane 7: Human kidney tissue lysate (30 ug/Lane) Lane 8: Human stomach tissue lysate (30 ug/Lane) Lane 9: Mouse large intestine tissue lysate (30 ug/Lane) Predicted band size: 44 kDa Observed band size: 55 kDa (Glycosylation) Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32715) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32715_rabbit-par2-mab-je35-05-278202416285258.jpg "Western Blot: PAR2 Antibody (JE35-05) [NBP3-32715] - Western blot analysis of PAR2 on different lysates with Rabbit anti-PAR2 antibody (NBP3-32715) at 1/1,000 dilution. Lane 1: HCT 116 cell lysate (15 ug/Lane) Lane 2: HepG2 cell lysate (15 ug/Lane) Lane 3: PANC-1 cell lysate (15 ug/Lane) Lane 4: PC-3M cell lysate (15 ug/Lane) Lane 5: AGS cell lysate (15 ug/Lane) Lane 6: SGC-7901 cell lysate (15 ug/Lane) Lane 7: Human kidney tissue lysate (30 ug/Lane) Lane 8: Human stomach tissue lysate (30 ug/Lane) Lane 9: Mouse large intestine tissue lysate (30 ug/Lane) Predicted band size: 44 kDa Observed band size: 55 kDa (Glycosylation) Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32715) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Flow Cytometry: PAR2 Antibody (JE35-05) [NBP3-32715] - Flow cytometric analysis of HepG2 cells labeling PAR2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32715, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32715_rabbit-par2-mab-je35-05-29202416453257.jpg)
![Immunohistochemistry-Paraffin: PAR2 Antibody [NBP1-44306] - Analysis of anti-F2RL1 antibody with human kidney.](http://images.novusbio.com/fullsize/PAR2-Antibody-Immunohistochemistry-Paraffin-NBP1-44306-img0002.jpg "Immunohistochemistry-Paraffin: PAR2 Antibody [NBP1-44306] - Analysis of anti-F2RL1 antibody with human kidney.")
![SDS-Page: Recombinant Human PAR2 Protein [H00002150-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-PAR2-Protein-SDS-Page-H00002150-Q01-img0002.jpg "SDS-Page: Recombinant Human PAR2 Protein [H00002150-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![ELISA: Rat PAR2 ELISA Kit (Colorimetric) [NBP3-06968] - Samples were spiked with high concentrations of Rat PAR2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-06968_rat-par2-elisa-kit-colorimetric-13220241613058.jpg "ELISA: Rat PAR2 ELISA Kit (Colorimetric) [NBP3-06968] - Samples were spiked with high concentrations of Rat PAR2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat PAR2 ELISA Kit (Colorimetric) [NBP3-06968] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-06968_rat-par2-elisa-kit-colorimetric-432024859733.jpg)
![ELISA: Human PAR2 ELISA Kit (Colorimetric) [NBP3-06966] - Samples were spiked with high concentrations of Human PAR2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-06966_human-par2-elisa-kit-colorimetric-132202416121319.jpg "ELISA: Human PAR2 ELISA Kit (Colorimetric) [NBP3-06966] - Samples were spiked with high concentrations of Human PAR2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human PAR2 ELISA Kit (Colorimetric) [NBP3-06966] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-06966_human-par2-elisa-kit-colorimetric-4320249163399.jpg)
![ELISA: Mouse PAR2 ELISA Kit (Colorimetric) [NBP3-06967] - Samples were spiked with high concentrations of Mouse PAR2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-06967_mouse-par2-elisa-kit-colorimetric-132202416254090.jpg "ELISA: Mouse PAR2 ELISA Kit (Colorimetric) [NBP3-06967] - Samples were spiked with high concentrations of Mouse PAR2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse PAR2 ELISA Kit (Colorimetric) [NBP3-06967] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-06967_mouse-par2-elisa-kit-colorimetric-432024973650.jpg)