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pan Actin Antibody (5J11) [DyLight 594]

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pan Actin Antibody (5J11) [DyLight 594] [NBP2-25142DL594] - Vial of DyLight 594 conjugated antibody. DyLight 594 is optimally excited at 593 nm by the Yellow-Green laser (561 nm) and has an emission maximum of 618 nm.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, EqSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
5J11
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 594

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pan Actin Antibody (5J11) [DyLight 594] Summary

Immunogen
An Actin preparation derived from bovine brain.
Localization
Cytoplasm > cytoskeleton.
Specificity
This antibody recognizes all six isotypes of Actin.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ACTA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for pan Actin Antibody (5J11) [DyLight 594]

  • ACTA
  • actin, alpha 1, skeletal muscle
  • alpha skeletal muscle actin
  • alpha skeletal muscle
  • alpha-actin-1
  • ASMA
  • CFTD
  • CFTDM
  • MPFD
  • NEM1
  • NEM2
  • NEM3

Background

Actins are highly conserved proteins ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. Actin has been found to be expressed in at least six isomeric forms. It is expressed in heart and skeletal striated muscle tissue, and in certain smooth muscle tissues, regulating contractile potentials for these cells. It is also expressed in the cytoplasm of non-muscle cells, functioning to control cell structure and motility (1). Polymerization of globular actin (G-actin) leads to a structural filament (F-actin), whose formation requires the Arp2/3 complex (2). It has been reported that actin is hyperphosphorylated in primary breast tumors (3). It has also been shown that actin dysfunction leads to certain types of heart failure (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for pan Actin Antibody (NBP2-25142DL594) (0)

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Secondary Antibodies

 

Isotype Controls

Additional pan Actin Products

Array NBP2-25142DL594

Research Areas for pan Actin Antibody (NBP2-25142DL594)

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Blogs on pan Actin.

"Actin the Fool" about Cytoskeleton Structure
Actins are highly conserved, commonly found and abundant proteins involved in several types of cell motility as well as cytoskeleton maintenance. In vertebrate species, three main groups of actin isoforms, the alpha, beta and gamma, have been identifi...  Read full blog post.

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Bioinformatics

Gene Symbol ACTA1