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PAG1 Antibody (PAG-C1) [DyLight 594]

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Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, IHC, IP
Clone
PAG-C1
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 594

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PAG1 Antibody (PAG-C1) [DyLight 594] Summary

Immunogen
C-terminal peptide (last 15 amino acids) of human Csk binding protein coupled to KLH.
Epitope
Located in the C-terminal domain.
Specificity
The antibody PAG-C1 recognizes an epitope located in the C-terminal domain of Csk-binding protein (Cbp / PAG), a 46 kDa ubiquitously expressed transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PAG1
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for PAG1 Antibody (PAG-C1) [DyLight 594]

  • CBP
  • CBPMGC138364
  • Csk-binding protein
  • PAG1
  • PAGFLJ37858
  • phosphoprotein associated with glycosphingolipid microdomains 1
  • phosphoprotein associated with glycosphingolipid-enriched microdomains 1
  • Transmembrane adapter protein PAG
  • Transmembrane phosphoprotein Cbp

Background

PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PAG1