![Western Blot: p38 gamma/SAPK3 Antibody (OTI10E1) - Azide and BSA Free [NBP2-73189] - hHEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAPK3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAPK3.](http://images.novusbio.com/fullsize/p38-gamma-SAPK3-Antibody-OTI10E1-Azide-and-BSA-Free-Western-Blot-NBP2-73189-img0001.jpg "Western Blot: p38 gamma/SAPK3 Antibody (OTI10E1) - Azide and BSA Free [NBP2-73189] - hHEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAPK3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAPK3.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, IHC, CyTOF-ready |
| Clone | OTI10E1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Full-length protein expressed in 293T cell transfected with human MAPK12 expression vector |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | MAPK12 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 42 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. |
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Secondary Antibodies |
Isotype Controls |
Research Areas for p38 gamma/SAPK3 Antibody (NBP2-73189)Find related products by research area.
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| Gene Symbol | MAPK12 |
![Immunohistochemistry: p38 gamma/SAPK3 Antibody (OTI10E1) - Azide and BSA Free [NBP2-73189] - Staining of paraffin-embedded Human pancreas tissue using anti-SAPK3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/p38-gamma-SAPK3-Antibody-OTI10E1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73189-img0003.jpg "Immunohistochemistry: p38 gamma/SAPK3 Antibody (OTI10E1) - Azide and BSA Free [NBP2-73189] - Staining of paraffin-embedded Human pancreas tissue using anti-SAPK3 mouse monoclonal antibody.")
![Flow Cytometry: p38 gamma/SAPK3 Antibody (OTI10E1) - Azide and BSA Free [NBP2-73189] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-SAPK3 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/p38-gamma-SAPK3-Antibody-OTI10E1-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73189-img0002.jpg "Flow Cytometry: p38 gamma/SAPK3 Antibody (OTI10E1) - Azide and BSA Free [NBP2-73189] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-SAPK3 antibody, and then analyzed by flow cytometry.")
![Immunohistochemistry: p38 gamma/SAPK3 Antibody (OTI10E1) - Azide and BSA Free [NBP2-73189] - Staining of paraffin-embedded Human breast tissue using anti-SAPK3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/p38-gamma-SAPK3-Antibody-OTI10E1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73189-img0004.jpg "Immunohistochemistry: p38 gamma/SAPK3 Antibody (OTI10E1) - Azide and BSA Free [NBP2-73189] - Staining of paraffin-embedded Human breast tissue using anti-SAPK3 mouse monoclonal antibody.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")