Home » p21/CIP1/CDKN1A » p21/CIP1/CDKN1A Antibodies » p21/CIP1/CDKN1A Antibody [Unconjugated]
Novus Biologicals products are now on bio-techne.com

p21/CIP1/CDKN1A Antibody [Unconjugated]

 (8 Publications)
 
 See All Related
Submit a Review

Images

 
(
Enlarge)
Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 1 µM camptothecin (CPT) for 16 hours. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human p21/CIP1/CDKN1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1047), followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for p21/CIP1/CDKN1A at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 1 µM camptothecin (CPT) for 16 hours. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human p21/CIP1/CDKN1A ...read more
p21/CIP1/CDKN1A was detected in immersion fixed MCF-7 human breast cancer cell line treated with (left panel) or without (right panel) camptothecin using Goat Anti-Human p21/CIP1/CDKN1A Antigen Affinity-purified ...read more
p21/CIP1/CDKN1A was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 1.7 µg/mL Goat Anti-Human p21/CIP1/CDKN1A Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
MCF‑7 human breast cancer cell line was unstimulated (light orange filled histogram) or treated with 1 μM camphtothecin for 16 hours, then stained with Goat Anti-Human p21/CIP1/CDKN1A Antigen Affinity‑purified ...read more
Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line untreated (-) or treated (+) with 1 µM Camptothecin (CPT) for 16 hours, loaded at 0.2 mg/mL. A specific band was detected for ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, IP, CyTOF-ready, Flow, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF1047
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF1047-SP
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

p21/CIP1/CDKN1A Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human p21
Ser2-Pro164
Accession # P38936
Specificity
Detects human p21 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CDKN1A
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 1-2 ug/500 ug cell lysate
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Simple Western 5 ug/mL
  • Western Blot 0.5 ug/mL
Publications
Read Publications using
AF1047 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for p21/CIP1/CDKN1A Antibody [Unconjugated]

  • CAP20cyclin-dependent kinase inhibitor 1
  • CDK-interacting protein 1
  • CDKN1A
  • CDKN1melanoma differentiation associated protein 6
  • CIP1
  • CIP1WAF1CDK-interaction protein 1
  • cyclin-dependent kinase inhibitor 1A (p21, Cip1)
  • MDA6
  • MDA-6
  • Melanoma differentiation-associated protein 6
  • p21
  • p21CIP1
  • p21Cip1/Waf1
  • PIC1
  • SDI1DNA synthesis inhibitor
  • wild-type p53-activated fragment 1

Background

p21CIP1, also called CIP1 (CDK-interacting protein 1) and CDKN1A, is a 21 kDa Cyclin/Cyclin-dependent kinase (Cdk) inhibitor that blocks cell cycle progression from G1 to S phase in the cell cycle. Because p21 is a transcriptional target of the p53 tumor suppressor, p21 expression increases in cells that contain stabilized p53 due to genotoxic stress exposure.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

p53 Antibody (PAb
240) - BSA Free
NB200-103
Flow Cytometry p53 Antibody (PAb 240) - BSA Free
Flow Cytometry p53 Antibody (PAb 240) - BSA Free
Flow (Intracellular) p53 Antibody (PAb 240) - BSA Free
(3 Reviews)
(42 Publications)
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
p19ARF/CDKN2A
Antibody - BSA Free
NB200-106
Immunohistochemistry p19ARF/CDKN2A Antibody - BSA Free
Western Blot p19ARF/CDKN2A Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence p19ARF/CDKN2A Antibody - BSA Free
(1 Review)
(48 Publications)
Species: Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, WB
p27/Kip1 [p
Thr157] Antibody
AF1555
Western Blot p27/Kip1 [p Thr157] Antibody
(1 Review)
(17 Publications)
Species: Hu
Applications: WB
CDK2 Antibody
(OTI2D9)
NBP2-00776
Flow Cytometry CDK2 Antibody (OTI2D9)
Flow Cytometry CDK2 Antibody (OTI2D9)
Flow Cytometry CDK2 Antibody (OTI2D9)
(1 Review)
Species: Ca, Hu, Pm, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
IFI27 Antibody
(CL14627)
NBP3-26876
Immunohistochemistry-Paraffin IFI27 Antibody (CL14627)
Immunohistochemistry-Paraffin IFI27 Antibody (CL14627)
Immunohistochemistry-Paraffin IFI27 Antibody (CL14627)
Species: Hu
Applications: IHC
26S proteasome
subunit 9 Antibody
NBP2-47560
Immunohistochemistry 26S proteasome subunit 9 Antibody
Immunohistochemistry-Paraffin 26S proteasome subunit 9 Antibody
Immunohistochemistry-Paraffin 26S proteasome subunit 9 Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Dynactin 6 Antibody
NBP1-80616
Immunohistochemistry-Paraffin Dynactin 6 Antibody
Western Blot Dynactin 6 Antibody
Western Blot Dynactin 6 Antibody
(2 Publications)
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
SSSCA1 Antibody
(OTI2F5)
NBP2-03198
Flow Cytometry SSSCA1 Antibody (OTI2F5)
Flow Cytometry SSSCA1 Antibody (OTI2F5)
Flow Cytometry SSSCA1 Antibody (OTI2F5)
(1 Publication)
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
TMED7 Antibody
NBP1-90949
Immunohistochemistry TMED7 Antibody
Immunohistochemistry TMED7 Antibody
Immunohistochemistry TMED7 Antibody
(1 Publication)
Species: Hu
Applications: IHC, IHC-P, WB
PCNA Antibody
(PC10)
NB500-106
Immunocytochemistry/ Immunofluorescence PCNA Antibody (PC10)
Western Blot PCNA Antibody (PC10)
Flow Cytometry PCNA Antibody (PC10)
(5 Reviews)
(45 Publications)
Species: Ch, Dr, Fi, Hu, Mar, Mu, Po, Pm, Rb, Rt, Ye, Ze
Applications: ChIP, ChIP, ELISA, Flow, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, IP, Simple Western, WB
Cyclin D1 Antibody
(SPM587)
NBP2-32840
Western Blot Cyclin D1 Antibody (SPM587)
Flow Cytometry Cyclin D1 Antibody (SPM587)
Flow Cytometry Cyclin D1 Antibody (SPM587)
(1 Review)
(9 Publications)
Species: Hu, Pm, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
CIB1 Antibody
(791119) [Unconjugate
MAB7557
Western Blot CIB1 Antibody (791119) [Unconjugated]
Immunohistochemistry CIB1 Antibody (791119) [Unconjugated]
(1 Publication)
Species: Hu
Applications: IHC, WB
CDK4 Antibody
NBP1-31308
Immunocytochemistry/ Immunofluorescence CDK4 Antibody
Immunocytochemistry/ Immunofluorescence CDK4 Antibody
Immunohistochemistry-Paraffin CDK4 Antibody
(7 Publications)
Species: Bv, Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
RB1 Antibody
(607121) [Unconjugate
MAB6495
Western Blot RB1 Antibody (607121) [Unconjugated]
Immunocytochemistry RB1 Antibody (607121) [Unconjugated]
Simple Western RB1 Antibody (607121) [Unconjugated]
(5 Publications)
Species: Hu
Applications: ICC, Simple Western, WB
Bcl-2 Antibody
NB100-56098
Western Blot Bcl-2 Antibody
Western Blot Bcl-2 Antibody
Western Blot Bcl-2 Antibody
(2 Reviews)
(26 Publications)
Species: Ca, Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB
Bax Antibody (6A7)
NBP1-28566
Western Blot Bax Antibody (6A7)
Western Blot Bax Antibody (6A7)
Immunohistochemistry-Frozen Bax Antibody (6A7)
(3 Reviews)
(20 Publications)
Species: Bv, Hu, Pm, Mu, Po, Rt
Applications: Func, IHC, IHC-Fr, IHC-P, WB
Caspase-3 Antibody
[Unconjugated] - Acti ...
AF835
Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active
Immunohistochemistry Caspase-3 Antibody [Unconjugated] - Active
Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active
(7 Reviews)
(387 Publications)
Species: Hu, Mu
Applications: ICC, IHC
ERK2 Antibody
[Unconjugated]
AF1230
Western Blot ERK2 Antibody [Unconjugated]
Immunohistochemistry ERK2 Antibody [Unconjugated]
Knockout Validated ERK2 Antibody [Unconjugated]
(3 Publications)
Species: Hu, Mu, Rt
Applications: IHC, KO, WB

Publications for p21/CIP1/CDKN1A Antibody (AF1047)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC, Western Blot.


Filter By Application
ICC
(2)
Western Blot
(2)
All Applications
Filter By Species
Human
(3)
All Species
Showing Publications 1 - 8 of 8.
Publications using AF1047 Applications Species
Taylor P Enrico, Wayne Stallaert, Elizaveta T Wick, Peter Ngoi, Xianxi Wang, Seth M Rubin et al. Cyclin F drives proliferation through SCF-dependent degradation of the retinoblastoma-like tumor suppressor p130/RBL2 eLife 12/1/2021 [PMID: 34851822]
JOR Hernandez, X Wang, M Vazquez-Se, M Lopez-Marf, MF Sobral-Rey, A Moran-Horo, M Sundberg, DO Lopez-Cant, CK Probst, GU Ruiz-Espar, K Giannikou, R Abdi, EP Henske, DJ Kwiatkowsk, M Sahin, DR Lemos A tissue-bioengineering strategy for modeling rare human kidney diseases in vivo Nature Communications, 2021-11-11;12(1):6496. 11/11/2021 [PMID: 34764250]
K Koltowska, KS Okuda, M Gloger, M Rondon-Gal, E Mason, J Xuan, S Dudczig, H Chen, H Arnold, R Skoczylas, NI Bower, S Paterson, AK Lagendijk, GJ Baillie, I Leshchiner, C Simons, KA Smith, W Goessling, JK Heath, RB Pearson, E Sanij, S Schulte-Me, BM Hogan The RNA helicase Ddx21 controls Vegfc-driven developmental lymphangiogenesis by balancing endothelial cell ribosome biogenesis and p53 function Nature Cell Biology, 2021-11-08;23(11):1136-1147. 2021-11-08 [PMID: 34750583] (ICC, Western Blot, Human) ICC, Western Blot Human
AJ Rodrigues, JJ Bandolik, FK Hansen, T Kurz, A Hamacher, MU Kassack Priming with HDAC Inhibitors Sensitizes Ovarian Cancer Cells to Treatment with Cisplatin and HSP90 Inhibitors Int J Mol Sci, 2020-11-05;21(21):. 2020-11-05 [PMID: 33167494] (Western Blot, Human) Western Blot Human
Jianjun Wang, Yongliang Yao, Qinghui Zhang, Shasha Li, Lijun Tang Inflammatory responses induced by Helicobacter pylori on the carcinogenesis of gastric epithelial GES‑1 cells International Journal of Oncology 4/9/2019 [PMID: 31081048]
Jenni Lahtela, Laura B. Corson, Annabrita Hemmes, Matthew J. Brauer, Sonja Koopal, James Lee et al. A high-content cellular senescence screen identifies candidate tumor suppressors, including EPHA3 Cell Cycle 2/15/2013 [PMID: 23324396]
Lucia Napione, Marina Strasly, Claudia Meda, Stefania Mitola, Maria Alvaro, Gabriella Doronzo et al. IL-12-dependent innate immunity arrests endothelial cells in G0-G1 phase by a p21(Cip1/Waf1)-mediated mechanism Angiogenesis 12/1/2012 [PMID: 22797886]
Jiang WQ, Zhong ZH, Nguyen A, Henson JD, Toouli CD, Braithwaite AW, Reddel RR Induction of alternative lengthening of telomeres-associated PML bodies by p53/p21 requires HP1 proteins. J. Cell Biol., 2009-05-25;185(5):797-810. 2009-05-25 [PMID: 19468068] (ICC, Human) ICC Human

Reviews for p21/CIP1/CDKN1A Antibody (AF1047) (0)

There are no reviews for p21/CIP1/CDKN1A Antibody (AF1047). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for p21/CIP1/CDKN1A Antibody (AF1047) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional p21/CIP1/CDKN1A Products

Blogs on p21/CIP1/CDKN1A

There are no specific blogs for p21/CIP1/CDKN1A, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our p21/CIP1/CDKN1A Antibody [Unconjugated] and receive a gift card or discount.


Bioinformatics

Gene Symbol CDKN1A
Uniprot