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CIB1 Antibody (791119) [Unconjugated]

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Western blot shows lysates of human kidney tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CIB1 Monoclonal Antibody (Catalog # MAB7557) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CIB1 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human kidney tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CIB1 Monoclonal Antibody (Catalog # MAB7557) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody ...read more
CIB1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human CIB1 Monoclonal Antibody (Catalog # MAB7557) at 15 µg/mL overnight at 4 °C. Before incubation with ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
791119
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB7557-SP
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CIB1 Antibody (791119) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human CIB1
Gly2-Leu191
Accession # E9Q3T6
Specificity
Detects human CIB1 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CIB1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 2 ug/mL
Publications
Read Publication using
MAB7557 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CIB1 Antibody (791119) [Unconjugated]

  • calcium and integrin binding 1 (calmyrin)
  • Calcium- and integrin-binding protein
  • Calmyrin
  • CIB1
  • CIBcalcium and integrin binding protein
  • CIBP
  • DNA-dependent protein kinase interacting protein
  • DNA-PKcs-interacting protein
  • Kinase-interacting protein
  • KIP
  • KIP1
  • KIPcalcium and integrin binding, protein kinase interacting protein
  • PRKDCIP
  • SIP2-28
  • SIP2-28calcium and integrin-binding protein 1
  • Snk interacting protein 2-28
  • SNK-interacting protein 2-28

Background

Calcium and Integrin binding protein 1 (CIB1), also known as Calmyrin, is a ubiquitously expressed 22 kDa myristoylated and membrane-associated protein. It contains two EF hand domains (aa 103-138 and aa 148-183). Calmyrin interacts with a range of proteins including Integrin alpha 2b and GPIIb/IIIa, Presenilin-2, InsP3R, Stathmin-2, Myosin 1C, Sphingosine Kinase 1, Polo-like kinases, and Rac3. Human Calmyrin shares 94% aa sequence identity with mouse and rat Calmyrin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CIB1 Antibody (MAB7557)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-P.


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Bioinformatics

Gene Symbol CIB1
Uniprot