Osteopontin/OPN Antibody (190312) [Unconjugated]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Osteopontin (OPN), previously called SPP1 (secreted phosphoprotein 1), Eta1(early T lymphocyte activation 1) or BSP (bone sialoprotein), is a secreted molecule in the SIBLING (small integrin binding ligand N-linked glycoprotein) family of noncollagenous matricellular proteins (1‑3). Human OPN is synthesized as a 317 aminoacid (aa) precursor protein with a 16 aa signal peptide and a 301 aa mature protein (3). Mature human OPN shares 64% and 62% aa sequence identity with mouse and rat OPN, respectively. OPN is highly acidic and has 26 potential Ser/Thr phosphorylation sites and a C‑terminal CD44 binding site (1‑4). Depending on tissue-specific modification by O- and N‑glycosylation, sulfation, phosphorylation and transglutamination, OPN can be detected at 45‑75 kDa (5, 6). The central region of OPN contains RGD and nonRGD binding sites for multiple integrins (3, 4). Adjacent to the RGD motif is the sequence SVVYGLR (SLAYGLR in mouse) which serves as a cryptic binding site for additional integrins: it is masked in full length OPN but is exposed following OPN cleavage by thrombin in tumors and sites of tissue injury
(6‑8). OPN can also be cleaved by MMP3, 7, 9, and 12 within the SVVYGLR motif and at sites closer to the C‑terminus (8, 9). OPN is widely expressed and is prominent in mineralized tissues. It inhibits bone mineralization and kidney stone formation, and promotes inflammation and cell adhesion and migration (1, 2, 4, 6). Its expression is up‑regulated during inflammation, obesity, atherosclerosis, cancer, and tissue damage, and contributes to the pathophysiology of these conditions (1, 2, 6, 9, 10).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 3 applications: ICC, IHC, IHC-P.

Showing Publications 1 - 6 of 6.

Publications using MAB1433	Applications	Species
M Fang, K Liu, X Li, Y Wang, W Li, B Li AntagomiR-29b inhibits vascular and valvular calcification and improves heart function in rats J. Cell. Mol. Med., 2020-08-26;0(0):. 2020-08-26 [PMID: 32845082] (IHC, Rat)	IHC	Rat
Serena E O'Neil, Carina Malmhäll, Konstantinos Samitas, Teet Pullerits, Apostolos Bossios, Jan Lötvall Quantitative expression of osteopontin in nasal mucosa of patients with allergic rhinitis: effects of pollen exposure and nasal glucocorticoid treatment Allergy, Asthma & Clinical Immunology 2010-12-01 [PMID: 21044308]
Samitas K, Zervas E, Vittorakis S, Semitekolou M, Alissafi T, Bossios A, Gogos H, Economidou E, Lotvall J, Xanthou G, Panoutsakopoulou V, Gaga M Osteopontin expression and relation to disease severity in human asthma. Eur. Respir. J., 2010-06-18;37(2):331-41. 2010-06-18 [PMID: 20562127] (IHC-P, Human)	IHC-P	Human
Xiao Wang, Lan Chao, Guohui Ma, Liansheng Chen, Guangchao Jin, Mei Hua et al. Primary Breast Carcinoma: Association of Mammographic Calcifications with Osteopontin Expression Radiology 2010-01-01 [PMID: 20019131]
Hofmann A, Ritz U, Verrier S, Eglin D, Alini M, Fuchs S, Kirkpatrick CJ, Rommens PM The effect of human osteoblasts on proliferation and neo-vessel formation of human umbilical vein endothelial cells in a long-term 3D co-culture on polyurethane scaffolds. Biomaterials, 2008-08-09;29(31):4217-26. 2008-08-09 [PMID: 18692894] (ICC, Human)	ICC	Human
Xanthou G, Alissafi T, Semitekolou M, Simoes DC, Economidou E, Gaga M, Lambrecht BN, Lloyd CM, Panoutsakopoulou V Osteopontin has a crucial role in allergic airway disease through regulation of dendritic cell subsets. Nat. Med., 2007-04-15;13(5):570-8. 2007-04-15 [PMID: 17435770] (IHC-P, Human)	IHC-P	Human

Images	Ratings	Applications	Species	Date	Details
	4 reviewed by: Verified Customer	IHC-P	Human	02/10/2015	View Summary Application Immunohistochemistry-Paraffin Sample Tested See PMID 20562127 Species Human

Array MAB1433

• Osteopontin/OPN Antibodies
• Osteopontin/OPN ELISA Kit
• Osteopontin/OPN Lysates
• Osteopontin/OPN Proteins
• Osteopontin/OPN ELISAs
• Osteopontin/OPN Proteins and Enzymes