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OLFM4 Antibody (806305) [Unconjugated]

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Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line and SW480 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Olfactomedin-4/OLFM4 Monoclonal Antibody (Catalog # MAB9218) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Olfactomedin-4/OLFM4 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line and SW480 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Olfactomedin-4/OLFM4 ...read more
Olfactomedin-4/OLFM4 was detected in immersion fixed Capan‑1 human pancreatic adenocarcinoma cell line (Positive) and absent in U937 human histiocytic lymphoma cell line (Negative) using Mouse Anti-Human ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clone
806305
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB9218-SP
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OLFM4 Antibody (806305) [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Olfactomedin-4/OLFM4
Asp21-Gln510
Accession # Q6UX06
Specificity
Detects human Olfactomedin-4/OLFM4 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 1-15 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for OLFM4 Antibody (806305) [Unconjugated]

  • Antiapoptotic protein GW112
  • bA209J19.1
  • GC1
  • G-CSF-stimulated clone 1 protein
  • GW112
  • GW112hOLfD
  • hGC-1
  • KIAA4294
  • olfactomedin 4
  • Olfactomedin-4
  • OlfD
  • OLFM4
  • OLM4
  • UNQ362

Background

Olfactomedin-4 (OLFM4), previously called GC-1 (G-CSF-stimulated clone-1), GW112 or hOlfD, is a 64 kDa secreted glycoprotein that is a member of the Olfactomedin/Noelin family of proteins. Mature Olfactomedin-4 is a 490 amino acid (aa) protein that is secreted mainly as multimers. Human Olfactomedin-4 is mainly expressed by gastrointestinal stem cells, developing granulocytes, type M4 acute myelogenous leukemias, and intestinal and prostate cancer cells and is upregulated by NF-kB signaling. It is reported to facilitate cell-cell adhesion by binding to lectins and cadherins, and to be anti-apoptotic. Mature human Olfactomedin-4 shares 71% aa sequence identity with mouse and rat Olfactomedin-4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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