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OCT4 Antibody [FITC]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Pm, Po, FeSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
FITC

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-2379AF350
Alexa Fluor 405
NB100-2379AF405
Alexa Fluor 488
NB100-2379AF488
Alexa Fluor 532
NB100-2379AF532
Alexa Fluor 594
NB100-2379AF594
Alexa Fluor 647
NB100-2379AF647
Alexa Fluor 700
NB100-2379AF700
Alexa Fluor 750
NB100-2379AF750
Allophycocyanin
NB100-2379APC
Biotin
NB100-2379B
CoraFluor 1
NB100-2379CL1
DyLight 350
NB100-2379UV
DyLight 405
NB100-2379V
DyLight 488
NB100-2379G
DyLight 550
NB100-2379R
DyLight 594
NB100-2379DL594
DyLight 594
NB100-2379PD6
DyLight 650
NB100-2379C
DyLight 680
NB100-2379FR
DyLight 755
NB100-2379IR
HRP
NB100-2379H
Janelia Fluor 525
NB100-2379JF525
Janelia Fluor 549
NB100-2379JF549
Janelia Fluor 585
NB100-2379JF585
Janelia Fluor 635
NB100-2379JF635
Janelia Fluor 646
NB100-2379JF646
Janelia Fluor 669
NB100-2379JF669
PE
NB100-2379PE
PerCP
NB100-2379PCP
Unconjugated
NB100-2379
mFluor Violet 450 SE
NB100-2379MFV450
mFluor Violet 500 SE
NB100-2379MFV500
mFluor Violet 610 SE
NB100-2379MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB100-2379
BSA Free
NB100-2379SS
BSA Free
NB100-2379
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OCT4 Antibody [FITC] Summary

Immunogen
A synthetic peptide made to an internal portion of the human OCT4 protein (between residues 100-200) [UniProt# Q01860]
Localization
Nuclear
Marker
Embryonic Stem Cell Marker
Predicted Species
Porcine (93%), Feline (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
POU5F1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Publications
Read Publications using
NB100-2379F in the following applications:

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Feline (93%), Porcine (93%). Customer feedback suggests that this antibody does not give good results in IHC-P on feline tissues. Bovine reactivity reported in scientific literature (PMID: 23054358).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for OCT4 Antibody [FITC]

  • class 5, transcription factor 1
  • MGC22487
  • Oct-3
  • OCT3Oct4
  • Oct-4
  • Octamer-binding protein 3
  • otc-4
  • OTF3POU domain class 5, transcription factor 1
  • OTF4
  • POU class 5 homeobox 1
  • POU-type homeodomain-containing DNA-binding protein

Background

OCT4 (octamer-binding protein 4) is a transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and generates a trimeric complex with SOX2 on DNA for controlling the expression of several genes (YES1, FGF4, UTF1, ZFP206 etc.) involved in embryonic development. Expressed mainly in the nucleus, OCT4 interacts with UBE2I, ZSCAN10, PKM, WWP2 etc. and OCT4-PKM interaction positively regulate OCT4's transcriptional activity. Once translated, its modifications such as sumoylation enhances the protein stability, DNA binding and transactivation activity and is required for enhanced YES1 expression. OCT4 undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation. OCT4 is found in oocytes and is zygotically expressed in pluripotent cells of pre-gastrulation embryos, and has been observed to be essential for early embryogenesis as well as for embryonic stem cell pluripotency. OCT4 is expressed in developing brain with highest levels in specific cell layers of the cortex, the olfactory bulb, hippocampus and cerebellum. Its expression decreases gradually after embryoid body formation, however, improper re-activation of OCT4 contributes to oncogenic processes and it has been implicated in certain malignancies such as breast and ovarian cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for OCT4 Antibody (NB100-2379F)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: FLOW, ICC/IF.


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FLOW
(2)
ICC/IF
(2)
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(2)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for OCT4 Antibody (NB100-2379F) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol POU5F1