![Western Blot: Nuclear Factor Erythroid Derived 2 Antibody (JE65-92) [NBP3-32646] - Western blot analysis of Nuclear Factor Erythroid Derived 2 on different lysates with Rabbit anti-Nuclear Factor Erythroid Derived 2 antibody (NBP3-32646) at 1/1,000 dilution. Lane 1: U-937 cell lysate (15 ug/Lane) Lane 2: K-562 cell lysate (15 ug/Lane) Lane 3: TF-1 cell lysate (15 ug/Lane) Lane 4: HeLa cell lysate (15 ug/Lane) Lane 5: HepG2 cell lysate (15 ug/Lane) Lane 6: Mouse testis tissue lysate (30ug/Lane) Lane 7: Rat testis tissue lysate (30ug/Lane) Predicted band size: 41 kDa Observed band size: 41 kDa Exposure time: 1 minute 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32646) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32646_rabbit-nuclear-factor-erythroid-derived-2-mab-je65-92-27820241524264.jpg "Western Blot: Nuclear Factor Erythroid Derived 2 Antibody (JE65-92) [NBP3-32646] - Western blot analysis of Nuclear Factor Erythroid Derived 2 on different lysates with Rabbit anti-Nuclear Factor Erythroid Derived 2 antibody (NBP3-32646) at 1/1,000 dilution. Lane 1: U-937 cell lysate (15 ug/Lane) Lane 2: K-562 cell lysate (15 ug/Lane) Lane 3: TF-1 cell lysate (15 ug/Lane) Lane 4: HeLa cell lysate (15 ug/Lane) Lane 5: HepG2 cell lysate (15 ug/Lane) Lane 6: Mouse testis tissue lysate (30ug/Lane) Lane 7: Rat testis tissue lysate (30ug/Lane) Predicted band size: 41 kDa Observed band size: 41 kDa Exposure time: 1 minute 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32646) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | JE65-92 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant protein within Human Nuclear Factor Erythroid Derived 2 aa 251-350 / 373. (Uniprot: Q16621) |
| Localization | Cytoplasm, Nucleus. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | NFE2 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 41 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
![Immunocytochemistry FosB/G0S3 Antibody [Unconjugated]](https://images.novusbio.com/images2/FosB_AF2214_Immunocytochemistry__Immunofluorescence_21634.jpg)
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Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | NFE2 |
![Immunohistochemistry: Nuclear Factor Erythroid Derived 2 Antibody (JE65-92) [NBP3-32646] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-Nuclear Factor Erythroid Derived 2 antibody (NBP3-32646) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32646) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32646_rabbit-nuclear-factor-erythroid-derived-2-mab-je65-92-298202414115440.jpg "Immunohistochemistry: Nuclear Factor Erythroid Derived 2 Antibody (JE65-92) [NBP3-32646] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-Nuclear Factor Erythroid Derived 2 antibody (NBP3-32646) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32646) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: Nuclear Factor Erythroid Derived 2 Antibody (JE65-92) [NBP3-32646] - Immunocytochemistry analysis of K-562 cells labeling Nuclear Factor Erythroid Derived 2 with Rabbit anti-Nuclear Factor Erythroid Derived 2 antibody (NBP3-32646) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Nuclear Factor Erythroid Derived 2 antibody (NBP3-32646) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32646_rabbit-nuclear-factor-erythroid-derived-2-mab-je65-92-29202410234219.jpg "Immunocytochemistry/ Immunofluorescence: Nuclear Factor Erythroid Derived 2 Antibody (JE65-92) [NBP3-32646] - Immunocytochemistry analysis of K-562 cells labeling Nuclear Factor Erythroid Derived 2 with Rabbit anti-Nuclear Factor Erythroid Derived 2 antibody (NBP3-32646) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Nuclear Factor Erythroid Derived 2 antibody (NBP3-32646) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.")
![Flow Cytometry: Nuclear Factor Erythroid Derived 2 Antibody (JE65-92) [NBP3-32646] - Flow cytometric analysis of K-562 cells labeling Nuclear Factor Erythroid Derived 2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32646, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32646_rabbit-nuclear-factor-erythroid-derived-2-mab-je65-92-29202416453280.jpg "Flow Cytometry: Nuclear Factor Erythroid Derived 2 Antibody (JE65-92) [NBP3-32646] - Flow cytometric analysis of K-562 cells labeling Nuclear Factor Erythroid Derived 2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32646, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")