![Western Blot: Nrf1 Antibody (NRF1/2609) [NBP3-07451] - Western Blot Analysis of human HEK293, HeLa, A431, MOLT4 cell lysates using Nrf1 Mouse Monoclonal Antibody (NRF1/2609).](http://images.novusbio.com/fullsize/Nrf1-Antibody-NRF1-2609-Western-Blot-NBP3-07451-img0003.jpg "Western Blot: Nrf1 Antibody (NRF1/2609) [NBP3-07451] - Western Blot Analysis of human HEK293, HeLa, A431, MOLT4 cell lysates using Nrf1 Mouse Monoclonal Antibody (NRF1/2609).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC |
| Clone | NRF1/2609 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08773) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Recombinant full-length human Nrf1 protein (Uniprot: Q16656) |
| Localization | Nucleus. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | NRF1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry-Paraffin: Nrf1 Antibody (NRF1/2609) [NBP3-07451] - Formalin-fixed, paraffin-embedded human prostate carcinoma stained with Nrf1 Mouse Monoclonal Antibody (Nrf1/2609).](http://images.novusbio.com/fullsize/Nrf1-Antibody-NRF1-2609-Immunohistochemistry-Paraffin-NBP3-07451-img0002.jpg "Immunohistochemistry-Paraffin: Nrf1 Antibody (NRF1/2609) [NBP3-07451] - Formalin-fixed, paraffin-embedded human prostate carcinoma stained with Nrf1 Mouse Monoclonal Antibody (Nrf1/2609).")
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