![Western Blot: NQO-1 Antibody (13A1-R) [NBP3-32659] - Western blot analysis of NQO-1 on different lysates with Mouse anti-NQO-1 antibody (NBP3-32659) at 1/2,000 dilution. Lane 1: A549 cell lysate Lane 2: SiHa cell lysate Lane 3: HepG2 cell lysate Lane 4: MCF7 cell lysate Lane 5: HeLa cell lysate Lane 6: Saos-2 cell lysate (low expression) Lysates/proteins at 30 ug/Lane. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32659) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32659_mouse-nqo-1-mab-13a1-r-27820241524265.jpg "Western Blot: NQO-1 Antibody (13A1-R) [NBP3-32659] - Western blot analysis of NQO-1 on different lysates with Mouse anti-NQO-1 antibody (NBP3-32659) at 1/2,000 dilution. Lane 1: A549 cell lysate Lane 2: SiHa cell lysate Lane 3: HepG2 cell lysate Lane 4: MCF7 cell lysate Lane 5: HeLa cell lysate Lane 6: Saos-2 cell lysate (low expression) Lysates/proteins at 30 ug/Lane. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32659) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
| Reactivity | HuSpecies Glossary |
| Applications | WB |
| Clone | 13A1-R |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant protein within Human NQO-1 aa 1-274 / 274. (Uniprot: P15559) |
| Localization | Cytoplasm. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | NQO1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 31 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH7.4), 0.1% BSA and 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | NQO1 |
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")