NQO-1 Antibodies
We offer NQO-1 Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, Sandwich ELISA, Simple Western, Western Blot. Each NQO-1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our NQO-1 Antibodies can be used in a variety of model species: Canine, Human, Mouse, Porcine, Primate, Rat. Use the list below to choose the NQO-1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our NQO-1 polyclonal antibodies and browse our NQO-1 monoclonal antibody catalog.
Alternate Names for NQO-1 Antibodies
anti-NQO-1 antibody, anti-NQO1 antibody, anti-Azoreductase antibody, anti-DHQU antibody, anti-DIA4azoreductase antibody, anti-diaphorase (NADH/NADPH) (cytochrome b-5 reductase) antibody, anti-dioxin-inducible 1 antibody, anti-DT-diaphorase antibody, anti-DTDEC 1.6.5.2 antibody, anti-Menadione reductase antibody, anti-NAD(P)H dehydrogenase [quinone] 1 antibody, anti-NAD(P)H dehydrogenase, quinone 1 antibody, anti-NAD(P)H:menadione oxidoreductase 1 antibody, anti-NAD(P)H:Quinone acceptor oxidoreductase type 1 antibody, anti-NAD(P)H:quinone oxidoreductase 1 antibody, anti-NAD(P)H:quinone oxireductase antibody, anti-NMOR1diaphorase-4 antibody, anti-Phylloquinone reductase antibody, anti-QR1NMORI antibody, anti-Quinone reductase 1 antibody
12 Results for "nqo-1" in Primary Antibodies
![Western Blot: NQO-1 Antibody (A180) [NB200-209] - Antioxidant enzyme synthesis in response to orange oil treatment. Immunoblot analysis demonstrating expression of NQO1 protein at 6, 12 and 24 hrs following 15 min treatment of BEAS-2B cells with the oil preparation or time-matched soy oil control. Representative blots from one of three separate experiments are shown above. Densitometric evaluations of each target protein blot normalized to its corresponding GAPDH for all three experiments are provided below. Bars represent mean +/- SEM. Image collected and cropped by CiteAb from the following publication (//respiratory-research.biomedcentral.com/articles/10.1186/1465-9921-12-92), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NQO-1-Antibody-A180-Western-Blot-NB200-209-img0019.jpg "<b>Biological Strategies Validation. </b>Western Blot: NQO-1 Antibody (A180) [NB200-209] - Antioxidant enzyme synthesis in response to orange oil treatment. Immunoblot analysis demonstrating expression of NQO1 protein at 6, 12 and 24 hrs following 15 min treatment of BEAS-2B cells with the oil preparation or time-matched soy oil control. Representative blots from one of three separate experiments are shown above. Densitometric evaluations of each target protein blot normalized to its corresponding GAPDH for all three experiments are provided below. Bars represent mean +/- SEM. Image collected and cropped by CiteAb from the following publication (//respiratory-research.biomedcentral.com/articles/10.1186/1465-9921-12-92), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: NQO-1 Antibody (A180) [NB200-209] - NQO1 antibody was tested in U2OS cells with Dylight 488 (green). Nuclei and beta-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/NQO-1-Antibody-A180-Immunocytochemistry-Immunofluorescence-NB200-209-img0010.jpg)
![Immunohistochemistry-Paraffin: NQO-1 Antibody (A180) [NB200-209] - Analysis of NQO1 in human breast cancer using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/NQO-1-Antibody-A180-Immunohistochemistry-Paraffin-NB200-209-img0014.jpg)
![Flow Cytometry: NQO-1 Antibody (A180) [NB200-209] - An intracellular stain was performed on U-87 cells with NQO-1 [A180] Antibody NB200-209AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/NQO-1-Antibody-A180-Flow-Cytometry-NB200-209-img0018.jpg)
![Western Blot: NQO-1 Antibody (A180) [NB200-209] - Detection of NQO1 in A549 lysates using NB 200-209. Lane 1: 0.5 ug/ml. Lane 2: 2 ug/ml. Exposure: 1 second.](http://images.novusbio.com/fullsize/NQO-1-Antibody-A180-Western-Blot-NB200-209-img0007.jpg)
![Immunohistochemistry-Paraffin: NQO-1 Antibody (A180) [NB200-209] - Immunohistochemistry showing Anti-NQ01 on formalin fixed paraffin embedded human kidney tissue](http://images.novusbio.com/fullsize/NQO-1-Antibody-A180-Immunohistochemistry-Paraffin-NB200-209-img0012.jpg)
![Flow (Intracellular): NQO-1 Antibody (A180) [NB200-209] - An intracellular stain was performed on U87MG cells with NQO1 (A180) antibody NBP2-24917 (blue) along with a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize/NQO-1-Antibody-A180-Flow-Intracellular-NB200-209-img0015.jpg)
![Flow Cytometry: NQO-1 Antibody (A180) [NB200-209] - Detection of NQO-1 in Human A549 Cell Line by Flow Cytometry. Human A549 cell line was stained with Mouse Anti- NQO-1 Monoclonal Antibody (Catalog # NB200-209, filled histogram), or Mouse IgG1 isotype control (Catalog # MAB002, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/fullsize/NQO-1-Antibody-A180-Flow-Cytometry-NB200-209-img0016.jpg)
![Flow Cytometry: NQO-1 Antibody (A180) [NB200-209] - An intracellular stain was performed on U-87 cells with NQO-1 [A180] Antibody NB200-209C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.](http://images.novusbio.com/fullsize/NQO-1-Antibody-A180-Flow-Cytometry-NB200-209-img0017.jpg)
![Simple Western: NQO-1 Antibody (A180) [NB200-209] - Simple Western lane view shows a specific band for NQO-1 in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/NQO-1-Antibody-A180-Simple-Western-NB200-209-img0013.jpg)
![Knockdown Validated: NQO-1 Antibody [NBP1-31355] - (b) Immunohistochemical analyses of RKO/pNQO1, RKO/shCont, and RKO/pshNQO1 xenograft tumours. The sections were stained for NQO1. Scale bar, 50 um. (c) Quantification of NQO1 in RKO/pNQO1, RKO/shCont and RKO/pshNQO1 xenograft tumours (n=3 each group). n=5 in each tumour. Two-tail t-test. *P<0.05. All error bars represent the mean +/- s.e.m. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms13593) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Knockdown-Validated-NBP1-31355-img0025.jpg "<b>Genetic Strategies Validation. </b>Knockdown Validated: NQO-1 Antibody [NBP1-31355] - (b) Immunohistochemical analyses of RKO/pNQO1, RKO/shCont, and RKO/pshNQO1 xenograft tumours. The sections were stained for NQO1. Scale bar, 50 um. (c) Quantification of NQO1 in RKO/pNQO1, RKO/shCont and RKO/pshNQO1 xenograft tumours (n=3 each group). n=5 in each tumour. Two-tail t-test. *P<0.05. All error bars represent the mean +/- s.e.m. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms13593) licensed under a CC-BY license.")
![<b>Genetic Strategies Validation. </b>Western Blot: NQO-1 Antibody [NBP1-31355] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NQO1 antibody [C2C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Western-Blot-NBP1-31355-img0021.jpg)
![Immunohistochemistry: NQO-1 Antibody [NBP1-31355] - IHC detection of high-level expression of NQO1 (n=11) compared with the low-level expression of NQO1 (n=9). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms13593) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Immunohistochemistry-NBP1-31355-img0024.jpg)
![Immunocytochemistry/Immunofluorescence: NQO-1 Antibody [NBP1-31355] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31355-img0016.jpg)
![Western Blot: NQO-1 Antibody [NBP1-31355] - A. 50 ug mouse heart lysate/extract 12% SDS-PAGE NQO1 antibody [C2C3], C-term dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Western-Blot-NBP1-31355-img0017.jpg)
![Western Blot: NQO-1 Antibody [NBP1-31355] - A. 50 ug rat heart lysate/extract 12% SDS-PAGE NQO1 antibody [C2C3], C-term (GTX100235) dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Western-Blot-NBP1-31355-img0018.jpg)
![Immunohistochemistry-Paraffin: NQO-1 Antibody [NBP1-31355] - Human rectal carcinoma. NQO1 antibody [C2C3], C-term diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Immunohistochemistry-Paraffin-NBP1-31355-img0019.jpg)
![Immunohistochemistry-Paraffin: NQO-1 Antibody [NBP1-31355] - Mouse kidney. NQO1 stained by NQO1 antibody [C2C3], C-term diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Immunohistochemistry-Paraffin-NBP1-31355-img0022.jpg)
![Immunohistochemistry-Paraffin: NQO-1 Antibody [NBP1-31355] - Mouse kidney. NQO1 stained by NQO1 antibody [C2C3], C-term diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Immunohistochemistry-Paraffin-NBP1-31355-img0023.jpg)
![Western Blot: NQO-1 Antibody [NB100-1005] - (0.3ug/ml) staining of U251 (A) and HepG2 (B) cell lysate. (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Western-Blot-NB100-1005-img0005.jpg "Western Blot: NQO-1 Antibody [NB100-1005] - (0.3ug/ml) staining of U251 (A) and HepG2 (B) cell lysate. (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: NQO-1 Antibody [NB100-1005] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/NQO-1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1005-img0001.jpg)
![Immunohistochemistry: NQO-1 Antibody [NB100-1005] - (2ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Immunohistochemistry-NB100-1005-img0002.jpg)
![Western Blot: NQO-1 Antibody [NB100-1005] - (0.3ug/ml) staining of Rat Kidney (A), Lung (B) and Duodenum (C) lysate. (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Western-Blot-NB100-1005-img0003.jpg)
![Western Blot: NQO-1 Antibody [NB100-1005] - (0.3ug/ml) staining of Human Kidney (A), Lung (B) and Duodenum (C) lysate. (35ug protein in RIPA buffer). Detected by chemiluminescence](http://images.novusbio.com/fullsize/NQO-1-Antibody-Western-Blot-NB100-1005-img0004.jpg)
![Immunohistochemistry-Paraffin: NQO-1 Antibody [NBP1-85223] - Staining in human stomach and lymph node tissues.. Corresponding NQO1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85223-img0014.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NQO-1 Antibody [NBP1-85223] - Staining in human stomach and lymph node tissues.. Corresponding NQO1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: NQO-1 Antibody [NBP1-85223] - Analysis in human cell lines A-549 and HEK293 using Anti-NQO1 antibody. Corresponding NQO1 RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Western-Blot-NBP1-85223-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: NQO-1 Antibody [NBP1-85223] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85223-img0008.jpg)
![Immunohistochemistry-Paraffin: NQO-1 Antibody [NBP1-85223] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85223-img0017.jpg)
![Immunohistochemistry-Paraffin: NQO-1 Antibody [NBP1-85223] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85223-img0015.jpg)
![Immunohistochemistry-Paraffin: NQO-1 Antibody [NBP1-85223] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85223-img0016.jpg)
![Immunohistochemistry-Paraffin: NQO-1 Antibody [NBP1-85223] - Staining of human lymph node shows no positivity in non - germinal center cells as expected.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85223-img0018.jpg)
![Western Blot: NQO-1 Antibody (1E3-A6) [H00001728-M01] - Analysis of NQO1 expression in transfected 293T cell line by NQO1 monoclonal antibody (M01), clone 1E3-A6.Lane 1: NQO1 transfected lysate(30.9 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/NQO-1-Antibody-1E3-A6-Western-Blot-H00001728-M01-img0013.jpg "Western Blot: NQO-1 Antibody (1E3-A6) [H00001728-M01] - Analysis of NQO1 expression in transfected 293T cell line by NQO1 monoclonal antibody (M01), clone 1E3-A6.Lane 1: NQO1 transfected lysate(30.9 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: NQO-1 Antibody (1E3-A6) [H00001728-M01] - Analysis of monoclonal antibody to NQO1 on HepG2 cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/NQO-1-Antibody-1E3-A6-Immunocytochemistry-Immunofluorescence-H00001728-M01-img0008.jpg)
![Western Blot: NQO-1 Antibody (1E3-A6) [H00001728-M01] - Analysis of NQO1 over-expressed 293 cell line, cotransfected with NQO1 Validated Chimera RNAi ( Cat # H00001728-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NQO1 monoclonal antibody (M01), clone 1E3-A6 (Cat # H00001728-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/NQO-1-Antibody-1E3-A6-Western-Blot-H00001728-M01-img0010.jpg)
![Western Blot: NQO-1 Antibody (1E3-A6) [H00001728-M01] - NQO1 monoclonal antibody (M01), clone 1E3-A6 Analysis of NQO1 expression in HepG2.](http://images.novusbio.com/fullsize/NQO-1-Antibody-1E3-A6-Western-Blot-H00001728-M01-img0012.jpg)
![Immunoprecipitation: NQO-1 Antibody (1E3-A6) [H00001728-M01] - Analysis of NQO1 transfected lysate using anti-NQO1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NQO1 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/NQO-1-Antibody-1E3-A6-Immunoprecipitation-H00001728-M01-img0009.jpg)
![Sandwich ELISA: NQO-1 Antibody (1E3-A6) [H00001728-M01] - Detection limit for recombinant GST tagged NQO1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NQO-1-Antibody-1E3-A6-Sandwich-ELISA-H00001728-M01-img0011.jpg)
![NQO-1 Antibody (1O2N3) [NBP3-15793] - Analysis of extracts of various lysates, using NQO1 antibody at 1:20000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-15793_rabbit-nqo-1-mab-1o2n3-knockout-validated-4520238114..jpg "NQO-1 Antibody (1O2N3) [NBP3-15793] - Analysis of extracts of various lysates, using NQO1 antibody at 1:20000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![NQO-1 Antibody (1O2N3) [NBP3-15793] - Analysis of extracts from wild type(WT) and NQO1 knockdown (KD) HeLa cells, using NQO1 antibody at 1:20000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-15793_rabbit-nqo-1-mab-1o2n3-knockdown-validated-45202381317..jpg)
![NQO-1 Antibody (1O2N3) [NBP3-15793] - Analysis of Rat stomach using NQO1 antibody at dilution of 1:300 (40x lens).Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15793_rabbit-nqo-1-mab-1o2n3-immunohistochemistry-4520238153..jpg)
![NQO-1 Antibody (1O2N3) [NBP3-15793] -Analysis of Mouse stomach using NQO1 antibody at dilution of 1:300 (40x lens).Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15793_rabbit-nqo-1-mab-1o2n3-immunohistochemistry-45202381637..jpg)
![Western Blot: NQO-1 Antibody (13A1) [NBP2-80395] - Analysis of NQO-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:5,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: SiHa cell lysate, untreated Lane 2: A549 cell lysate, untreated](http://images.novusbio.com/fullsize/NQO-1-Antibody-13A1-Western-Blot-NBP2-80395-img0002.jpg "Western Blot: NQO-1 Antibody (13A1) [NBP2-80395] - Analysis of NQO-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:5,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: SiHa cell lysate, untreated Lane 2: A549 cell lysate, untreated")
![Immunohistochemistry-Paraffin: NQO-1 Antibody (13A1) [NBP2-80395] - Immunohistochemical analysis of paraffin-embedded rat uterus tissue using anti-NQO-1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 m](http://images.novusbio.com/fullsize/NQO-1-Antibody-13A1-Immunohistochemistry-Paraffin-NBP2-80395-img0003.jpg)
![Immunohistochemistry-Paraffin: NQO-1 Antibody (13A1) [NBP2-80395] - Analysis in human kidney tissue using NQO-1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with](http://images.novusbio.com/fullsize/NQO-1-Antibody-13A1-Immunohistochemistry-Paraffin-NBP2-80395-img0001.jpg)
![Western Blot: NQO-1 Antibody [NBP2-19573] - Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Western-Blot-NBP2-19573-img0002.jpg "Western Blot: NQO-1 Antibody [NBP2-19573] - Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: NQO-1 Antibody (002) [NBP2-90044] - Lane A: A549 Whole Cell Lysate. Lane B: MCF7 Whole Cell Lysate. Lane C: Raji Whole Cell Lysate. Lane D: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:31 kDa. Observed band size:31 kDa.](http://images.novusbio.com/fullsize/NQO-1-Antibody-002-Western-Blot-NBP2-90044-img0001.jpg "Western Blot: NQO-1 Antibody (002) [NBP2-90044] - Lane A: A549 Whole Cell Lysate. Lane B: MCF7 Whole Cell Lysate. Lane C: Raji Whole Cell Lysate. Lane D: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:31 kDa. Observed band size:31 kDa.")
![Western Blot: NQO-1 Antibody (13A1-R) [NBP3-32659] - Western blot analysis of NQO-1 on different lysates with Mouse anti-NQO-1 antibody (NBP3-32659) at 1/2,000 dilution. Lane 1: A549 cell lysate Lane 2: SiHa cell lysate Lane 3: HepG2 cell lysate Lane 4: MCF7 cell lysate Lane 5: HeLa cell lysate Lane 6: Saos-2 cell lysate (low expression) Lysates/proteins at 30 ug/Lane. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32659) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32659_mouse-nqo-1-mab-13a1-r-27820241524265.jpg "Western Blot: NQO-1 Antibody (13A1-R) [NBP3-32659] - Western blot analysis of NQO-1 on different lysates with Mouse anti-NQO-1 antibody (NBP3-32659) at 1/2,000 dilution. Lane 1: A549 cell lysate Lane 2: SiHa cell lysate Lane 3: HepG2 cell lysate Lane 4: MCF7 cell lysate Lane 5: HeLa cell lysate Lane 6: Saos-2 cell lysate (low expression) Lysates/proteins at 30 ug/Lane. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32659) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")