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NPY2R Antibody

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Western Blot: NPY2R Antibody [NB100-57847] - Staining of Rat Brain lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Rt, Hu, Mu, CaSpecies Glossary
Applications WB, ELISA, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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NPY2R Antibody Summary

Immunogen
Peptide with sequence C-KAKKNLEVKKNN corresponding to internal region according to NP_032757.1.
Epitope
KAKKNLEVKKNN
Specificity
Please note this antibody was designed using the Mouse sequence NP_032757.1, which differs by 1 amino acid from the Human sequence NP_000901.1.
Predicted Species
Human (100%), Mouse (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NPY2R
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoprecipitation 1:50
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.1 - 0.3 ug/mL
Application Notes
A customer has resported successful usage for IP of human Y2R, but the Human Y2R could not be detected in WB by this antibody. WB: Approx. 40-45 kDa band observed in rat brain lysates (calculated MW of 42.6 kDa band according to NP_032757.1).
Publications
Read Publications using NB100-57847.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NPY2R Antibody

  • neuropeptide Y receptor type 2
  • neuropeptide Y receptor Y2
  • NPY2R
  • NPY2-R
  • NPY-Y2 receptor
  • Y2 Receptor

Background

NPY2R is widely expressed in the central nervous system, with particularly high abundance in the hypothalamus. The hypothalamus is known to be important for appetite regulation and thus variants in this gene could be associated with obesity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NPY2R Antibody (NB100-57847)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for NPY2R Antibody (NB100-57847). (Showing 1 - 1 of 1 FAQs).

  1. Which cell lysate should I use with Anti- human Neuropeptide Y2R (cat# NB100-57847)?
    • NPY2R: High levels in amygdala, corpus callosum, hippocampus and subthalamic nucleus. Also detectable in caudate nucleus, hypothalamus and substantia nigra.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NPY2R
Entrez
Uniprot