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non-muscle heavy chain 10 Myosin Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: non-muscle heavy chain 10 Myosin Antibody [NBP2-38566] - Analysis in human placenta and skeletal muscle tissues. Corresponding MYH10 RNA-seq data are ...read more
Orthogonal Strategies: Western Blot: non-muscle heavy chain 10 Myosin Antibody [NBP2-38566] - Analysis in human cell lines HEK293 and PC-3 using anti-MYH10 antibody. Corresponding MYH10 RNA-seq data are presented ...read more
Immunohistochemistry-Paraffin: non-muscle heavy chain 10 Myosin Antibody [NBP2-38566] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: non-muscle heavy chain 10 Myosin Antibody [NBP2-38566] - Staining of human placenta shows moderate membranous positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: non-muscle heavy chain 10 Myosin Antibody [NBP2-38566] - Staining of human cerebellum shows weak to moderate cytoplasmic positivity in Purkinje cells.
Immunohistochemistry-Paraffin: non-muscle heavy chain 10 Myosin Antibody [NBP2-38566] - Staining of human kidney shows moderate membranous positivity in cells in tubules.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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non-muscle heavy chain 10 Myosin Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: RQLDDATEANEGLSREVSTLKNRLRRGGPISFSSSRSGRRQLHLEGASLELSDDDTESKTSDVNETQPPQ
Predicted Species
Mouse (96%), Rat (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MYH10
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
non-muscle heavy chain 10 Myosin Protein (NBP2-38566PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for non-muscle heavy chain 10 Myosin Antibody

  • cellular myosin heavy chain, type B type B
  • Cellular myosin heavy chain, type B
  • heavy polypeptide 10, non-muscle
  • MGC134913
  • MGC134914
  • MYH10
  • Myosin heavy chain 10
  • Myosin heavy chain, non-muscle IIb
  • myosin heavy chain, nonmuscle type B
  • myosin, heavy chain 10, non-muscle
  • myosin-10
  • near to the ATP binding region
  • NMMHC II-b
  • NMMHCB
  • NMMHC-B
  • NMMHC-IIB
  • non-muscle heavy chain 10 Myosin
  • Non-muscle myosin heavy chain B
  • nonmuscle myosin heavy chain IIB
  • Non-muscle myosin heavy chain IIb
  • nonmuscle myosin heavy chain-B
  • nonmuscle myosin II heavy chain-B

Background

Myosin II is a hexameric protein that consist of a pair of heavy chain subunits (MHC), a pair of essential light chain subunits (MLC), and a pair of regulatory light chain subunits (RLCs). Non-muscle myosin heavy chain II-B is encoded by the myosin heavy chain 10 gene (MYH10) and non-muscle myosin heavy chain II-A is encoded by the Myosin heavy chain 9 gene (MYH9). Myosin in non-muscle cells is involved in motile cellular processes which include cytokinesis, cell shape, secrection, and capping. Alternate names for Myosin-10 is Myosin heavy chain II-B, cellular myosin heavy chain type B, and NMMHCB.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol MYH10
Uniprot