![Western Blot: Nodal Antibody (5C3) [H00004838-M03] - Analysis of NODAL expression in Raw 264.7 (Cat # L024V1).](http://images.novusbio.com/fullsize/Nodal-Antibody-5C3-Western-Blot-H00004838-M03-img0012.jpg "Western Blot: Nodal Antibody (5C3) [H00004838-M03] - Analysis of NODAL expression in Raw 264.7 (Cat # L024V1).")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clone | 5C3 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Quality control test: Antibody Reactive Against Recombinant Protein. |
| Immunogen | NODAL (NP_060525, 275 a.a. ~ 346 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RCEGECPNPVGEEFHPTNHAYIQSLLKRYQPHRVPSTCCAPVKTKPLSMLYVDNGRVLLDHHKDMIVEECGC |
| Localization | Secreted |
| Specificity | NODAL - nodal homolog (mouse) |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | NODAL |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
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| Publication using H00004838-M03 | Applications | Species |
|---|---|---|
| Lawrence MG, Margaryan NV, Loessner D et al. Reactivation of embryonic nodal signaling is associated with tumor progression and promotes the growth of prostate cancer cells. Prostate. 2011-01-12 [PMID: 21229563] |
Secondary Antibodies |
Isotype Controls |
Research Areas for Nodal Antibody (H00004838-M03)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: Nodal Antibody (5C3) [H00004838-M03] - Analysis of monoclonal antibody to NODAL on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/Nodal-Antibody-5C3-Immunocytochemistry-Immunofluorescence-H00004838-M03-img0008.jpg "Immunocytochemistry/Immunofluorescence: Nodal Antibody (5C3) [H00004838-M03] - Analysis of monoclonal antibody to NODAL on HeLa cell. Antibody concentration 10 ug/ml")
![Immunohistochemistry-Paraffin: Nodal Antibody (5C3) [H00004838-M03] - Analysis of monoclonal antibody to NODAL on formalin-fixed paraffin-embedded human endometrium cancer. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/Nodal-Antibody-5C3-Immunohistochemistry-Paraffin-H00004838-M03-img0009.jpg "Immunohistochemistry-Paraffin: Nodal Antibody (5C3) [H00004838-M03] - Analysis of monoclonal antibody to NODAL on formalin-fixed paraffin-embedded human endometrium cancer. Antibody concentration 3 ug/ml")
![Western Blot: Nodal Antibody (5C3) [H00004838-M03] - Analysis of NODAL expression in HeLa (Cat # L013V1).](http://images.novusbio.com/fullsize/Nodal-Antibody-5C3-Western-Blot-H00004838-M03-img0010.jpg "Western Blot: Nodal Antibody (5C3) [H00004838-M03] - Analysis of NODAL expression in HeLa (Cat # L013V1).")
![Western Blot: Nodal Antibody (5C3) [H00004838-M03] - Analysis of NODAL expression in PC-12 (Cat # L012V1).](http://images.novusbio.com/fullsize/Nodal-Antibody-5C3-Western-Blot-H00004838-M03-img0011.jpg "Western Blot: Nodal Antibody (5C3) [H00004838-M03] - Analysis of NODAL expression in PC-12 (Cat # L012V1).")
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