NLRP3/NALP3 Antibody - BSA Free

NACHT, LRR and PYD domains-containing protein 3 (NALP3), Nucleotide-Binding Oligomerization Domain, or Leucine Rich Repeat and Pyrin Domain Containing 3 (NLRP3) (human NLRP3-isoform 2 theoretical molecular weight 118kDa) acts as a cytosolic receptor for stress-signals initiated by a wide variety of pathogen-associated molecular patterns (PAMPS) and danger-associated molecular patterns (DAMPs) (1). NLRP3 belongs to the family of NOD-like receptors, a type of pattern-recognition receptor (PRR) which participates in innate immune responses. The exact mechanisms leading to NLRP3 activation are still not fully resolved. Some proposed mechanisms for NLRP3 inflammasome activation include induced ion channel flux as exemplified by potassium efflux, sodium influx and calcium signaling, generation of mitochondrial reactive oxygen species (ROS), and lysosomal destabilization (2). Activation of NLRP3 induces its association with the adaptor protein Apoptosis-associated Speck-like protein containing CARD (ASC) forming a complex that binds to Caspase-1 (1, 2). The inflammasome complex, comprised of NLRP3, ASC and Caspase 1, induces the processing of pro-IL-1beta and pro-IL-18 and release of the mature inflammatory cytokines IL-1beta and IL-18 and pyroptosis (1-3).

NLRP3 is expressed in a variety of cell types such as monocytes, dendritic cells, lymphocytes and epithelial cells (3). Abnormal NLRP3 activation may occur as the result of inherited mutations and is associated with diseases such as hereditary periodic fevers (HPFs) and familial cold autoinflammatory syndrome (FCAS). Additionally, abnormal NLRP3 activation is associated with a variety of diseases such as gout, obesity, atherosclerosis, Alzheimers, multiple sclerosis and type 2 diabetes (1, 3). NLRP3 inflammasome is regulated by several post-translational modifications (e.g., ubiquitination, phosphorylation and sumoylation) as well as by various interacting proteins (e.g., JNK1, FBXO3, TXNIP, MARK4 and PKD) (4).

References

1. Abderrazak, A., Syrovets, T., Couchie, D., El Hadri, K., Friguet, B., Simmet, T., & Rouis, M. (2015). NLRP3 inflammasome: From a danger signal sensor to a regulatory node of oxidative stress and inflammatory diseases. Redox Biology. https://doi.org/10.1016/j.redox.2015.01.008

2. Yang, Y., Wang, H., Kouadir, M., Song, H., & Shi, F. (2019). Recent advances in the mechanisms of NLRP3 inflammasome activation and its inhibitors. Cell Death and Disease. https://doi.org/10.1038/s41419-019-1413-8

3. Zahid, A., Li, B., Kombe, A. J. K., Jin, T., & Tao, J. (2019). Pharmacological inhibitors of the nlrp3 inflammasome. Frontiers in Immunology. https://doi.org/10.3389/fimmu.2019.02538

4. Kelley, N., Jeltema, D., Duan, Y., & He, Y. (2019). The NLRP3 inflammasome: An overview of mechanisms of activation and regulation. International Journal of Molecular Sciences. https://doi.org/10.3390/ijms20133328

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, E. coli.

We have publications tested in 7 applications: ICC/IF, IF/IHC, IHC-P, IP, In Vivo, PAGE, WB.

Showing Publications 1 - 10 of 31. Show All 31 Publications. Collapse Publications.

Publications using NBP1-77080	Applications	Species
Wu F, Wei H, Hu Y et al. Upregulation of P2X7 Exacerbates Myocardial Ischemia-Reperfusion Injury through Enhancing Inflammation and Apoptosis in Diabetic Mice Journal of immunology (Baltimore, Md. : 1950) 2023-05-05 [PMID: 37144844] (WB, Mouse) Details: 1:1000 WB dilution	WB	Mouse
Yu Z, Yazdanpanah G, Alam J et al. Induction of Innate Inflammatory Pathways in the Corneal Epithelium in the Desiccating Stress Dry Eye Model Investigative ophthalmology & visual science 2023-04-03 [PMID: 37036417] (WB, Mouse)	WB	Mouse
Gupta A, Singh K, Fatima S et al. Neutrophil Extracellular Traps Promote NLRP3 Inflammasome Activation and Glomerular Endothelial Dysfunction in Diabetic Kidney Disease Nutrients 2022-07-20 [PMID: 35889923] (WB, Mouse) Details: 1:1000 WB diltuion	WB	Mouse
Chen XC, Wu D, Wu HL et al. Metformin improves renal injury of MRL/lpr lupus-prone mice via the AMPK/STAT3 pathway Lupus Science & Medicine 2022-04-11 [PMID: 35414608] (In Vivo)	In Vivo
Jin L, Jin F, Guo S et al. Metformin Inhibits NLR Family Pyrin Domain Containing 3 (NLRP)-Relevant Neuroinflammation via an Adenosine-5-Monophosphate-Activated Protein Kinase (AMPK)-Dependent Pathway to Alleviate Early Brain Injury After Subarachnoid Hemorrhage in Mice Frontiers in Pharmacology 2022-03-17 [PMID: 35370693] (IF/IHC, Mouse)	IF/IHC	Mouse
Gaul S, Schaeffer Km, Opitz L Et Al. Extracellular NLRP3 inflammasome particles are internalized by human coronary artery smooth muscle cells and induce pro-atherogenic effects Scientific reports 2021-07-26 [PMID: 34312415] (WB)	WB
Schultz V, Barrie JA, Donald CL et al. Oligodendrocytes are susceptible to Zika virus infection in a mouse model of perinatal exposure: Implications for CNS complications Glia 2021-08-01 [PMID: 33942402]
Yu, C, Chen, P Et al. hADSCs derived extracellular vesicles inhibit NLRP3inflammasome activation and dry eye. Sci Rep 2020-09-03 [PMID: 32884023] (WB, Mouse)	WB	Mouse
Verma V, Kumar P, Gupta S et al. Alpha-Hemolysin of uropathogenic E. coli regulates NLRP3 inflammasome activation and mitochondrial dysfunction in THP-1 macrophages Sci Rep 2020-07-28 [PMID: 32724079] (IP, WB, Human)	IP, WB	Human
Toyohara T, Roudnicky F, Florido MHC et al. Patient hiPSCs Identify Vascular Smooth Muscle Arylacetamide Deacetylase as Protective against Atherosclerosis Cell Stem Cell 2020-05-14 [PMID: 32413331] (IHC-P, Mouse)	IHC-P	Mouse
Kim H, Seo Js, Lee Sy et Al. AIM2 inflammasome contributes to brain injury and chronic post-stroke cognitive impairment in mice Brain Behav. Immun. 2020-03-19 [PMID: 32201254] (WB, Mouse)	WB	Mouse
Liang F, Huang T, Li B et al. High-intensity interval training and moderate-intensity continuous training alleviate beta-amyloid deposition by inhibiting NLRP3 inflammasome activation in APPswe/PS1dE9 mice Neuroreport 2020-03-25 [PMID: 32150150]
Verma V, Gupta S, Kumar P et al. Involvement of NLRP3 and NLRC4 Inflammasome in Uropathogenic E. coli Mediated Urinary Tract Infections Front Microbiol 2019-09-26 [PMID: 31551961] (IP, WB, PAGE, E. coli)	IP, WB, PAGE	E. coli
Liu JX, Yang C, Zhang WH et al. Disturbance of mitochondrial dynamics and mitophagy in sepsis-induced acute kidney injury Life Sci. 2019-08-31 [PMID: 31479679] (IHC-P, Mouse)	IHC-P	Mouse
Geyfman M, Debabov D, Poloso N, Alvandi N. Mechanistic Insight into the Activity of a Sulfone Compound Dapsone on Propionibacterium (Newly Reclassified as Cutibacterium) Acnes-Mediated Cytokine Production Exp. Dermatol. 2018-12-26 [PMID: 30585659] (WB, Human)	WB	Human
Pan Z, Shan Q, Gu P, Wang XM. miRNA-23a/CXCR4 regulates neuropathic pain via directly targeting TXNIP/NLRP3 inflammasome axis. J Neuroinflammation. 2018-01-31 [PMID: 29386025] (WB, Mouse)	WB	Mouse
Zhuang J, Wen X, Zhang YQ et al. TDP-43 upregulation mediated by the NLRP3 inflammasome induces cognitive impairment in 2 2',4,4'-tetrabromodiphenyl ether (BDE-47)-treated mice. Brain Behav. Immun. 2017-05-19 [PMID: 28532818]
Hua X, Yuan X et al. Desiccating stress worsens alkali burn injury by magnifying caspase-8-induced imbalance of NLRP3 and NLRP6. J Allergy Clin Immunol 2017-01-10 [PMID: 28479336] (IF/IHC, Mouse)	IF/IHC	Mouse
Chi W, Hua X, Chen X et al. Mitochondrial DNA oxidation induces imbalanced activity of NLRP3/NLRP6 inflammasomes by activation of caspase-8 and BRCC36 in dry eye. J. Autoimmun. 2017-02-23 [PMID: 28238526]
Lee HI, Lee SW, Kim NG et al. Low-level light emitting diode (LED) therapy suppresses inflammasome-mediated brain damage in experimental ischemic stroke. J Biophotonics 2017-02-06 [PMID: 28164443]
MacPherson Maximilian, Westbom Catherine, Kogan Helen, Shukla Arti Actin polymerization plays a significant role in asbestos-induced inflammasome activation in mesothelial cells in vitro. Histochem Cell Biol. 2016-12-24 [PMID: 28013367] (ICC/IF, Human)	ICC/IF	Human
Zhu W, Cao FS, Feng J et al. NLRP3 inflammasome activation contributes to long-term behavioral alterations in mice injected with lipopolysaccharide. Neuroscience. 2016-12-05 [PMID: 27923741] (WB, Mouse)	WB	Mouse
Lu C, Xu W, Shao J et al. Nrf2 Activation Is Required for Ligustrazine to Inhibit Hepatic Steatosis in Alcohol-Preferring Mice and Hepatocytes Toxicol. Sci 2017-02-01 [PMID: 27837167] (Mouse)		Mouse
Westbom Catherine, Thompson Joyce K, Leggett Alan et al. Inflammasome Modulation by Chemotherapeutics in Malignant Mesothelioma. PloS One 2015-01-01 [PMID: 26689911] (WB, Mouse)	WB	Mouse
Chi W, Chen H, Li F et al. HMGB1 promotes the activation of NLRP3 and caspase-8 inflammasomes via NF-kB pathway in acute glaucoma. J Neuroinflammation. 2015-07-30 [PMID: 26224068] (WB, Mouse)	WB	Mouse
Fann DY, Santro T, Manzanero S et al. Intermittent fasting attenuates inflammasome activity in ischemic stroke. Exp. Neurol. 2014-05-05 [PMID: 24805069] (WB, Mouse)	WB	Mouse
Yang-Wei Fann D, Lee SY et al. Intravenous immunoglobulin suppresses NLRP1 and NLRP3 inflammasome-mediated neuronal death in ischemic stroke. Cell Death Dis. 2013-09-05 [PMID: 24008734] (WB, Mouse)	WB	Mouse
Kanneganti TD, Ozoren N, Body-Malapel M et al. Bacterial RNA and small antiviral compounds activate caspase-1 through cryopyrin/Nalp3. Nature 2006-03-09 [PMID: 16407888]
L Agostini et al. NALP3 forms an IL-1beta-processing inflammasome with increased activity in Muckle-Wells autoinflammatory disorder. Immunity 20, 319. 2004-01-01 [PMID: 15030775]
O'Connor W Jr, Hartin JA, Zhu X et al. Cutting edge: CIAS1/cryopyrin/PYPAF1/NALP3/CATERPILLER 1.1 is an inducible inflammatory mediator with NF-kappa B suppressive properties. J. Immunol. 171:6329-33. 2003-01-01 [PMID: 14662828]
Chamaillard M, Girardin SE, Viala J et al. Nods, Nalps and Naip: intracellular regulators of bacterial-induced inflammation. Cell. Microbiol. 5:581-92. 2003-01-01 [PMID: 12925128]
Show All 31 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	4 reviewed by: Max MacPherson	IHC-P	Mouse	11/05/2015	View Summary Application Immunohistochemistry-Paraffin Sample Tested mouse lung Species Mouse Lot A-2
	4 reviewed by: Verified Customer	IF	Mouse	04/03/2015	View Summary Application Immunofluorescence Sample Tested corneal epithelium in mouse Species Mouse

Array NBP1-77080

• NLRP3/NALP3 Antibodies
• NLRP3/NALP3 ELISA Kit
• NLRP3/NALP3 Lysates
• NLRP3/NALP3 Proteins