![Immunocytochemistry/Immunofluorescence: NKX6.1 Antibody [NBP1-82553] - Comparison of the hiPSC differentiation outcome according to the stage of encapsulation. Whole mount immunofluorescence of encapsulated cells stained for insulin (green), NKX6.1 (red), PDX1 (purple) and DAPI (blue), gamma correction 0.4. Scale bars: 10 um. Graphs data are shown as mean +/- SEM. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-57305-x), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NKX6.1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82553-img0017.jpg "Immunocytochemistry/Immunofluorescence: NKX6.1 Antibody [NBP1-82553] - Comparison of the hiPSC differentiation outcome according to the stage of encapsulation. Whole mount immunofluorescence of encapsulated cells stained for insulin (green), NKX6.1 (red), PDX1 (purple) and DAPI (blue), gamma correction 0.4. Scale bars: 10 um. Graphs data are shown as mean +/- SEM. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-57305-x), licensed under a CC-BY license.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | Flow, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: PLGTHNPGGLKPPATGGLSSLGSPPQQLSAATPHGINDILSRPSM |
| Predicted Species | Rat (96%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | NKX6-1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for NKX6.1 Antibody (NBP1-82553)Find related products by research area.
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COVID-19 and metabolic dysregulation: SARS-CoV-2 injures human exocrine and endocrine pancreas Jamshed Arslan, Pharm D, PhD Humans rely on the pancreas for digesting food and generating energy from it. SARS-CoV-2-mediated damage to the exocrine pancreas is evident from the pancreatitis, pancreatic enlargeme... Read full blog post. |
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![Immunohistochemistry-Paraffin: NKX6.1 Antibody [NBP1-82553] - Staining of human colon shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/NKX6.1-Antibody-Immunohistochemistry-Paraffin-NBP1-82553-img0015.jpg "Immunohistochemistry-Paraffin: NKX6.1 Antibody [NBP1-82553] - Staining of human colon shows no positivity in glandular cells as expected.")
![Immunocytochemistry/Immunofluorescence: NKX6.1 Antibody [NBP1-82553] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NKX6.1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82553-img0013.jpg "Immunocytochemistry/Immunofluorescence: NKX6.1 Antibody [NBP1-82553] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.")
![Immunocytochemistry/Immunofluorescence: NKX6.1 Antibody [NBP1-82553] - Wnt-modulation influences the distribution of mono-hormonal and bi-hormonal S7 cells. IF analysis of NKX6.1+ (red), PDX1+ (green) cells and NKX6.1+/PDX1+ cells (yellow). Scale bar upper panel: 25 um, lower panel: 7.5 um. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fendo.2019.00293/full), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NKX6.1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82553-img0018.jpg "Immunocytochemistry/Immunofluorescence: NKX6.1 Antibody [NBP1-82553] - Wnt-modulation influences the distribution of mono-hormonal and bi-hormonal S7 cells. IF analysis of NKX6.1+ (red), PDX1+ (green) cells and NKX6.1+/PDX1+ cells (yellow). Scale bar upper panel: 25 um, lower panel: 7.5 um. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fendo.2019.00293/full), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: NKX6.1 Antibody [NBP1-82553] - Immunohistochemical staining of human pancreas shows strong nuclear positivity in islets of Langerhans.](http://images.novusbio.com/fullsize/NKX6.1-Antibody-Immunohistochemistry-Paraffin-NBP1-82553-img0010.jpg "Immunohistochemistry-Paraffin: NKX6.1 Antibody [NBP1-82553] - Immunohistochemical staining of human pancreas shows strong nuclear positivity in islets of Langerhans.")
![Immunohistochemistry-Paraffin: NKX6.1 Antibody [NBP1-82553] - Staining of human liver shows no positivity as expected.](http://images.novusbio.com/fullsize/NKX6.1-Antibody-Immunohistochemistry-Paraffin-NBP1-82553-img0014.jpg "Immunohistochemistry-Paraffin: NKX6.1 Antibody [NBP1-82553] - Staining of human liver shows no positivity as expected.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")