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NGLY1 Antibody

Images

 
Immunohistochemistry-Paraffin: NGLY1 Antibody [NBP1-83792] - Staining of human kidney shows moderate to strong granular cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: NGLY1 Antibody [NBP1-83792] - Staining of human lymph node shows moderate to strong granular cytoplasmic positivity in lymphoid cells.
Immunohistochemistry-Paraffin: NGLY1 Antibody [NBP1-83792] - Staining of human prostate shows moderate to strong granular cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: NGLY1 Antibody [NBP1-83792] - Staining of human testis shows moderate to strong granular cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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NGLY1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: ISDEDFLLLELLHWFKEEFFHWVNNVLCSKCGGQTRSRDRSLLPSDDELKWGAKEVEDHYCDACQFSNRFPRYNNPEKLLETRCGR
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NGLY1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
NGLY1 Protein (NBP1-83792PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (87%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for NGLY1 Antibody

  • EC 3.5.1.52
  • hPNGase
  • N-glycanase 1FLJ12409
  • peptide:N-glycanase
  • peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase
  • PNG1FLJ11005
  • PNGase

Background

NGLY1 is a highly conserved enzyme that catalyzes deglycosylation of misfolded N-linked glycoproteins by cleaving the glycan chain before the proteins are degraded by the proteasome (Zhou et al., 2006).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NGLY1