Home » NGFRAP1/BEX3/NADE » NGFRAP1/BEX3/NADE Antibodies » NGFRAP1/BEX3/NADE Antibody (672911) [Unconjugated]
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NGFRAP1/BEX3/NADE Antibody (672911) [Unconjugated]

 (1 Publication)
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Western blot shows lysates of HT-29 human colon adenocarcinoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human NGFRAP1/BEX3 Monoclonal Antibody (Catalog # MAB6858) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NGFRAP1/BEX3 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HT-29 human colon adenocarcinoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human ...read more
NGFRAP1/BEX3 was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human NGFRAP1/BEX3 Monoclonal Antibody (Catalog # MAB6858) at 15 µg/mL overnight at 4 °C. Before incubation ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC
Clone
672911
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB6858-SP
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NGFRAP1/BEX3/NADE Antibody (672911) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human NGFRAP1/BEX3
Met1-Pro111
Accession # Q00994
Specificity
Detects human NGFRAP1/BEX3 in direct ELISAs.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
NGFRAP1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publication using
MAB6858 in the following applications:

  • ICC
    1 publication
  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NGFRAP1/BEX3/NADE Antibody (672911) [Unconjugated]

  • BEX3
  • BEX3p75NTR-associated cell death executor
  • Brain-expressed X-linked protein 3
  • DXS6984EX-linked 3
  • HGR74
  • NADE
  • nerve growth factor receptor (TNFRSF16) associated protein 1
  • Nerve growth factor receptor-associated protein 1
  • NGFRAP1
  • Ovarian granulosa cell 13.0 kDa protein HGR74
  • ovarian granulosa cell protein (13kD)
  • protein BEX3

Background

NGFRAP1 (nerve growth factor receptor associated protein 1), also called BEX3 (brain-expressed X-linked protein 3), NADE (p75NTR-associated death executor) or FAP-1 (Fas-associated phosphatase-1), is a 24 kDa member of the BEX protein family. A nuclear export signal (aa 77-87) allows NGFRAP1 to export bound p75NTR. It is widely expressed, but highest in ovarian granulosa cells, testis, prostate, seminal vesicle and liver. Except for a 13-19 amino acid (aa) rodent Arg/His region at aa 35 that is not found in humans, human NGFRAP1 shares 94% aa identity with mouse and rat NGFRAP1. In humans, a 101 aa isoform with an alternative start site at aa 11 has been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NGFRAP1/BEX3/NADE Antibody (MAB6858)(1)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ICC, IHC.


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Bioinformatics

Gene Symbol NGFRAP1
Uniprot