![Western Blot: NGFR/TNFRSF16/p75NTR Antibody (SA39-02) [NBP2-67296] - Analysis of p75 NGF Receptor on PC-12 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/NGFR-TNFRSF16-p75NTR-Antibody-SA39-02-Western-Blot-NBP2-67296-img0011.jpg "Western Blot: NGFR/TNFRSF16/p75NTR Antibody (SA39-02) [NBP2-67296] - Analysis of p75 NGF Receptor on PC-12 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IP |
| Clone | SA39-02 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Synthetic peptide within Human NGFR/TNFRSF16/p75NTR aa 345-394 / 427. (SwissProt: P08138 Human; SwissProt: Q9Z0W1 Mouse; SwissProt: P07174 Rat) |
| Localization | Membrane |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | NGFR |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | IP: Use at an assay dependent concentration. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
![Immunohistochemistry TrkB Antibody [Unconjugated]](https://images.novusbio.com/images2/TrkB_AF1494_Immunohistochemistry_10167.jpg)
![Western Blot TrkB Antibody [Unconjugated]](https://images.novusbio.com/images2/TrkB_AF1494_Western_Blot_23433.jpg)
![Western Blot TrkB Antibody [Unconjugated]](https://images.novusbio.com/images2/TrkB_AF1494_Western_Blot_19007.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for NGFR/TNFRSF16/p75NTR Antibody (NBP2-67296)Find related products by research area.
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| Gene Symbol | NGFR |
![Immunocytochemistry/Immunofluorescence: NGFR/TNFRSF16/p75NTR Antibody (SA39-02) [NBP2-67296] - Staining NGFR p75 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NGFR-TNFRSF16-p75NTR-Antibody-SA39-02-Immunocytochemistry-Immunofluorescence-NBP2-67296-img0005.jpg "Immunocytochemistry/Immunofluorescence: NGFR/TNFRSF16/p75NTR Antibody (SA39-02) [NBP2-67296] - Staining NGFR p75 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: NGFR/TNFRSF16/p75NTR Antibody (SA39-02) [NBP2-67296] - Rat uterus tissue using anti-p75 NGF Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/NGFR-TNFRSF16-p75NTR-Antibody-SA39-02-Immunohistochemistry-Paraffin-NBP2-67296-img0009.jpg "Immunohistochemistry-Paraffin: NGFR/TNFRSF16/p75NTR Antibody (SA39-02) [NBP2-67296] - Rat uterus tissue using anti-p75 NGF Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot: NGFR/TNFRSF16/p75NTR Antibody (SA39-02) [NBP2-67296] - Western blot analysis of NGFR/TNFRSF16/p75NTR on skeletal muscle tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperatur](http://images.novusbio.com/fullsize/NGFR-TNFRSF16-p75NTR-Antibody-SA39-02-Western-Blot-NBP2-67296-img0010.jpg "Western Blot: NGFR/TNFRSF16/p75NTR Antibody (SA39-02) [NBP2-67296] - Western blot analysis of NGFR/TNFRSF16/p75NTR on skeletal muscle tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperatur")
![Immunocytochemistry/Immunofluorescence: NGFR/TNFRSF16/p75NTR Antibody (SA39-02) [NBP2-67296] - Staining NGFR p75 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/NGFR-TNFRSF16-p75NTR-Antibody-SA39-02-Immunocytochemistry-Immunofluorescence-NBP2-67296-img0001.jpg "Immunocytochemistry/Immunofluorescence: NGFR/TNFRSF16/p75NTR Antibody (SA39-02) [NBP2-67296] - Staining NGFR p75 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: NGFR/TNFRSF16/p75NTR Antibody (SA39-02) [NBP2-67296] - Analysis of mouse uterus tissue using anti-p75 NGF Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/NGFR-TNFRSF16-p75NTR-Antibody-SA39-02-Immunohistochemistry-Paraffin-NBP2-67296-img0006.jpg "Immunohistochemistry-Paraffin: NGFR/TNFRSF16/p75NTR Antibody (SA39-02) [NBP2-67296] - Analysis of mouse uterus tissue using anti-p75 NGF Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry-Paraffin: NGFR/TNFRSF16/p75NTR Antibody (SA39-02) [NBP2-67296] - Human tonsil tissue using anti-p75 NGF Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/NGFR-TNFRSF16-p75NTR-Antibody-SA39-02-Immunohistochemistry-Paraffin-NBP2-67296-img0007.jpg "Immunohistochemistry-Paraffin: NGFR/TNFRSF16/p75NTR Antibody (SA39-02) [NBP2-67296] - Human tonsil tissue using anti-p75 NGF Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry-Paraffin: NGFR/TNFRSF16/p75NTR Antibody (SA39-02) [NBP2-67296] - Human uterus tissue using anti-p75 NGF Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/NGFR-TNFRSF16-p75NTR-Antibody-SA39-02-Immunohistochemistry-Paraffin-NBP2-67296-img0008.jpg "Immunohistochemistry-Paraffin: NGFR/TNFRSF16/p75NTR Antibody (SA39-02) [NBP2-67296] - Human uterus tissue using anti-p75 NGF Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![N/A BDNF [HRP]](https://images.novusbio.com/images2/DATA_BDNF_DBD00_ELISA_587.jpg)
![N/A BDNF [HRP]](https://images.novusbio.com/images2/DATA_BDNF_DBD00_ELISA_588.jpg)
![N/A BDNF [HRP]](https://images.novusbio.com/images2/BDNF_DBD00_ELISA_36.jpg)
![Immunohistochemistry TrkA Antibody [Unconjugated]](https://images.novusbio.com/images2/TrkA_AF1056_Immunohistochemistry_6672.jpg)
![Western Blot TrkA Antibody [Unconjugated]](https://images.novusbio.com/images2/TrkA_AF1056_Western_Blot_17300.jpg)
![Immunohistochemistry TrkA Antibody [Unconjugated]](https://images.novusbio.com/images/af1056_rat-trka-affinity-purified-polyclonal-ab-8220249503264.jpg)
![N/A TNF RII/TNFRSF1B [HRP]](https://images.novusbio.com/images2/TNF_RII_DRT200_ELISA_176.jpg)
![N/A TNF RII/TNFRSF1B [HRP]](https://images.novusbio.com/images2/DATA_TNF_RII_DRT200_ELISA_817.jpg)
![N/A TNF RII/TNFRSF1B [HRP]](https://images.novusbio.com/images2/DATA_TNF_RII_DRT200_ELISA_818.jpg)
![Western Blot LEDGF Antibody (762815) [Unconjugated]](https://images.novusbio.com/images2/12543.jpg)
![Immunocytochemistry LEDGF Antibody (762815) [Unconjugated]](https://images.novusbio.com/images2/12613.jpg)
![Neutralization Siglec-7/CD328 Antibody [Unconjugated]](https://images.novusbio.com/images2/Siglec-7_AF1138_Block_Neutralize_8760.jpg)
![SDS-PAGE beta-NGF [Unconjugated]](https://images.novusbio.com/images2/beta-NGF_256-GF_321.jpg)
![Bioactivity beta-NGF [Unconjugated]](https://images.novusbio.com/images2/beta-NGF_256-GF_322.jpg)
![NT-3 [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "NT-3 [Unconjugated]")
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![Western Blot TrkC Antibody [Unconjugated]](https://images.novusbio.com/images2/TrkC_AF1404_Western_Blot_19346.jpg)
![Simple Western TrkC Antibody [Unconjugated]](https://images.novusbio.com/images2/TrkC_AF1404_Simple_Western_20169.jpg)
![Immunocytochemistry/ Immunofluorescence TrkC Antibody [Unconjugated]](https://images.novusbio.com/images/af1404_mouse-rat-trkc-affinity-purified-polyclonal-ab-8220249503256.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")