![Western Blot: NEU2 Antibody (4F4) [NBP2-00783] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEU2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEU2.](http://images.novusbio.com/fullsize/NEU2-Antibody-4F4-Western-Blot-NBP2-00783-img0002.jpg "Western Blot: NEU2 Antibody (4F4) [NBP2-00783] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEU2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEU2.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, ICC/IF |
| Clone | OTI4F4 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | Full length human recombinant protein of human NEU2 (NP_005374) produced in HEK293T cell. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | NEU2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 42.1 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
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| Publication using NBP2-00783 | Applications | Species |
|---|---|---|
| Akyildiz Demir S, Seyrantepe V. Identification of cytoplasmic sialidase NEU2-associated proteins by LC-MS/MS Turkish Journal of Biochemistry 2018-08-03 [PMID: 30214522] |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: NEU2 Antibody (4F4) [NBP2-00783] Staining of COS7 cells transiently transfected by pCMV6-ENTRY NEU2.](http://images.novusbio.com/fullsize/NEU2-Antibody-4F4-Immunocytochemistry-Immunofluorescence-NBP2-00783-img0003.jpg "Immunocytochemistry/Immunofluorescence: NEU2 Antibody (4F4) [NBP2-00783] Staining of COS7 cells transiently transfected by pCMV6-ENTRY NEU2.")
![Flow Cytometry: NEU2 Antibody (4F4) [NBP2-00783] - Analysis of Hela cells, using anti-NEU2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/NEU2-Antibody-4F4-Flow-Cytometry-NBP2-00783-img0001.jpg "Flow Cytometry: NEU2 Antibody (4F4) [NBP2-00783] - Analysis of Hela cells, using anti-NEU2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
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