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NEK2 Antibody (3B7)

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Western Blot: NEK2 Antibody (3B7) [H00004751-M11] - Analysis of NEK2 expression in NIH/3T3 (Cat # L018V1).
Western Blot: NEK2 Antibody (3B7) [H00004751-M11] - Analysis of NEK2 expression in NIH/3T3 (Cat # L018V1).
Immunocytochemistry/ Immunofluorescence: NEK2 Antibody (3B7) [H00004751-M11] - Analysis of monoclonal antibody to NEK2 on HeLa cell. Antibody concentration 25 ug/ml
Western Blot: NEK2 Antibody (3B7) [H00004751-M11] - Analysis of NEK2 expression in PC-12 (Cat # L012V1).
Western Blot: NEK2 Antibody (3B7) [H00004751-M11] - Analysis of NEK2 expression in Raw 264.7 (Cat # L024V1).
Western Blot: NEK2 Antibody (3B7) [H00004751-M11] - Analysis of NEK2 expression in MCF-7 (Cat # L046V1).
ELISA: NEK2 Antibody (3B7) [H00004751-M11] - Detection limit for recombinant GST tagged NEK2 is approximately 3ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
3B7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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NEK2 Antibody (3B7) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
NEK2 (AAH43502, 331 a.a. ~ 445 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EQELCVRERLAEDKLARAENLLKNYSLLKERKFLSLASNPELLNLPSSVIKKKVHFSGESKENIMRSENSESQLTSKSKCKDLKKRLHAAQLRAQALSDIEKNYQLKSRQILGMR
Specificity
NEK2 (3B7)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NEK2
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NEK2 Antibody (3B7)

  • EC 2.7.11
  • EC 2.7.11.1
  • HSPK 21
  • HsPK21
  • NEK2
  • NEK2AHsPK21
  • Never in mitosis A-related kinase 2
  • NIMA (never in mitosis gene a)-related kinase 2
  • NimA-like protein kinase 1
  • NimA-related protein kinase 2
  • NLK1
  • NLK1serine/threonine-protein kinase Nek2

Background

Protein kinase that is involved in mitotic regulation. May have a role at the G2-M transition. May also play a role in meiosis. Isoform 1 but not isoform 2 appears to play a role in centrosome splitting. Isoform 1 phosphorylates and activates NEK11 in G1/S-arrested cells. Isoform 2, which is not present in the nucleolus, does not

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol NEK2
Entrez
OMIM
Uniprot