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Recombinant Human NDE1 His Protein

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SDS-Page: Recombinant Human NDE1 Protein [NBP1-51090] - 15 % SDS-PAGE (3ug)
SDS-Page: Recombinant Human NDE1 Protein [NBP1-51090] - 15 % SDS-PAGE (3ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.5 mg/ml

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Recombinant Human NDE1 His Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-335 of Human NDE1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MEDSGKTFSS EEEEANYWKD LAMTYKQRAE NTQEELREFQ EGSREYEAEL ETQLQQIETR NRDLLSENNR LRMELETIKE KFEVQHSEGY RQISALEDDL AQTKAIKDQL QKYIRELEQA NDDLERAKRA TIMSLEDFEQ RLNQAIERNA FLESELDEKE NLLESVQRLK DEARDLRQEL AVQQKQEKPR TPMPSSVEAE RTDTAVQATG SVPSTPIAHR GPSSSLNTPG SFRRGLDDST GGTPLTPAAR ISALNIVGDL LRKVGALESK LASCRNLVYD QSPNRTGGPA SGRSSKNRDG GERRPSSTSV PLGDKGLDTS CRWLSKSTTR SSSSC
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
NDE1
Purity
>85%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • SDS-Page
Theoretical MW
39.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH 8.0), 20% glycerol, 5mM DTT, 0.2 M NaCl
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>85%, by SDS-PAGE

Alternate Names for Recombinant Human NDE1 His Protein

  • FLJ20101
  • HOM-TES-87
  • nuclear distribution protein nudE homolog 1
  • nudE nuclear distribution gene E homolog 1 (A. nidulans)
  • NudE
  • NUDE1
  • rat homolog

Background

NDE1 is a cytoplasmic protein belonging to the nudE protein family. Phosphorylated during mitosis, it is essential for the formation and function of the mitotic spindle in M phase and functions to regulate the Dynein-mediated transport of kinetochore proteins, as well as centrosome duplication during interphase. NDE1 is thought to interact with NDEL1, LIS1 and Dynein IC1/2, cytosolic in a signaling pathway that regulates the formation of neurons and is fundamental to the development of the cerebral cortex. Recombinant human NDE1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol NDE1