Nudel Antibody (OTI5E11) Summary
Immunogen |
Full length human recombinant protein of human NDEL1(NP_001020750) produced in HEk293 cell. |
Isotype |
IgG2a |
Clonality |
Monoclonal |
Host |
Mouse |
Gene |
NDEL1 |
Purity |
Immunogen affinity purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Flow Cytometry 1:100
- Immunocytochemistry/ Immunofluorescence 1:100
- Immunohistochemistry 1:150
- Immunohistochemistry-Paraffin 1:150
- Western Blot 1:500-2000
|
Theoretical MW |
36.9 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Packaging, Storage & Formulations
Storage |
Store at -20C. Avoid freeze-thaw cycles. |
Buffer |
PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
Preservative |
0.02% Sodium Azide |
Concentration |
0.6 mg/ml |
Purity |
Immunogen affinity purified |
Alternate Names for Nudel Antibody (OTI5E11)
Background
This gene encodes a thiol-activated peptidase that is phosphorylated in M phase of the cell cycle. Phosphorylation regulates the cell cycle-dependent distribution of this protein, with a fraction of the protein bound strongly to centrosomes in interphase and localized to mitotic spindles in early M phase. Overall, this protein plays a role in nervous system development. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq].
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
Video Protocols
FAQs for Nudel Antibody (NBP2-01086). (Showing 1 - 1 of 1 FAQ).
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I want to buy NBP2-01086, but I am confused by the antibody application. Does IHC means IHC-Frozen? I need this antibody who can be applied to IHC-Fr.
- No, this antibody has not been tested for IHC-Fr application in our lab, however, as it worked well in IHC-P (which has more tough conditions for antigens-antibody reactivity compared to IHC-Fr) as well as ICC-IF, we strongly believe that this antibody should work in IHC-Fr application also. Moreover, if you chose to test this antibody in IHC-Fr, you may qualify for our Innovator's Reward program.
Secondary Antibodies
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