Novus Biologicals products are now on bio-techne.com

Nudel Antibody (OTI5E11)

Images

 
Western Blot: Nudel Antibody (5E11) [NBP2-01086] Analysis of extracts (35ug) from 9 different cell lines by using anti-Nudel monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Nudel Antibody (5E11) [NBP2-01086] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Nudel.
Immunohistochemistry-Paraffin: Nudel Antibody (5E11) [NBP2-01086] - Staining of paraffin-embedded Human Ovary tissue using anti-Nudel mouse monoclonal antibody.
Flow Cytometry: Nudel Antibody (5E11) [NBP2-01086] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Nudel antibody, and then analyzed ...read more
Western Blot: Nudel Antibody (5E11) [NBP2-01086] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Nudel (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunohistochemistry-Paraffin: Nudel Antibody (5E11) [NBP2-01086] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Nudel mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Nudel Antibody (5E11) [NBP2-01086] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Nudel mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Nudel Antibody (5E11) [NBP2-01086] - Staining of paraffin-embedded Human Kidney tissue using anti-Nudel mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Nudel Antibody (5E11) [NBP2-01086] - Staining of paraffin-embedded Human liver tissue using anti-Nudel mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI5E11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.6 mg/ml

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Nudel Antibody (OTI5E11) Summary

Immunogen
Full length human recombinant protein of human NDEL1(NP_001020750) produced in HEk293 cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
NDEL1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
Theoretical MW
36.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.6 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Nudel Antibody (OTI5E11)

  • DKFZp451M0318
  • EOPA
  • MITAP1mitosin-associated protein MITAP1
  • Mitosin-associated protein 1
  • nuclear distribution protein nudE-like 1
  • nudE nuclear distribution gene E homolog (A. nidulans)-like 1
  • nudE nuclear distribution gene E homolog like 1 (A. nidulans)
  • nudE nuclear distribution gene E homolog like 1
  • NUDELendooligopeptidase A
  • NUDE-like protein
  • Protein Nudel

Background

This gene encodes a thiol-activated peptidase that is phosphorylated in M phase of the cell cycle. Phosphorylation regulates the cell cycle-dependent distribution of this protein, with a fraction of the protein bound strongly to centrosomes in interphase and localized to mitotic spindles in early M phase. Overall, this protein plays a role in nervous system development. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-87769
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NB110-40773
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P
DPLG70
Species: Hu
Applications: ELISA
H00054820-M01
Species: Hu, Rt
Applications: ELISA, ICC/IF, S-ELISA, WB
NBP2-15843
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KO, WB
NBP1-83908
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NB100-53816
Species: Hu, Rt
Applications: IHC, IHC-P, PEP-ELISA, WB
NBP2-01171
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NB500-101
Species: Bv, Eq, Hu, Mu
Applications: Flow, ICC/IF, IHC-WhMt, IHC, IHC-Fr, IHC-P, IP, KD, WB
NB100-852
Species: Hu, Mu
Applications: PEP-ELISA, WB
NBP1-89993
Species: Hu
Applications: IHC, IHC-P, WB
MAB4467
Species: Hu
Applications: IHC, KO, Simple Western, WB
NBP3-15638
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, WB
2428-FC
Species: Hu
Applications: Bind
NBP1-81838
Species: Hu, Mu
Applications: IHC, IHC-P, WB
NBP1-89827
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-92564
Species: Hu
Applications: ICC/IF, IHC, IHC-P
MAB7918
Species: Hu
Applications: Simple Western, WB

Publications for Nudel Antibody (NBP2-01086) (0)

There are no publications for Nudel Antibody (NBP2-01086).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Nudel Antibody (NBP2-01086) (0)

There are no reviews for Nudel Antibody (NBP2-01086). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Nudel Antibody (NBP2-01086). (Showing 1 - 1 of 1 FAQ).

  1. I want to buy NBP2-01086, but I am confused by the antibody application. Does IHC means IHC-Frozen? I need this antibody who can be applied to IHC-Fr.
    • No, this antibody has not been tested for IHC-Fr application in our lab, however, as it worked well in IHC-P (which has more tough conditions for antigens-antibody reactivity compared to IHC-Fr) as well as ICC-IF, we strongly believe that this antibody should work in IHC-Fr application also. Moreover, if you chose to test this antibody in IHC-Fr, you may qualify for our Innovator's Reward program.

Secondary Antibodies

 

Isotype Controls

Additional Nudel Products

Research Areas for Nudel Antibody (NBP2-01086)

Find related products by research area.

Blogs on Nudel

There are no specific blogs for Nudel, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Nudel Antibody (OTI5E11) and receive a gift card or discount.

Bioinformatics

Gene Symbol NDEL1
Entrez