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N-Acetylglucosaminyltransferase V/MGAT5 Antibody (706824)

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Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human N-Acetylglucosaminyltransferase V/MGAT5 Monoclonal Antibody (Catalog # MAB5469) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for N-Acetylglucosaminyltransferase V/MGAT5 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human N-Acetylglucosaminyltransferase V/MGAT5 Monoclonal Antibody (Catalog # MAB5469) ...read more
N-Acetylglucosaminyltransferase V/MGAT5 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human N-Acetylglucosaminyltransferase V/MGAT5 Monoclonal Antibody (Catalog # MAB5469) at 25 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clone
706824
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB5469-SP
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N-Acetylglucosaminyltransferase V/MGAT5 Antibody (706824) Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human N-Acetylglucosaminyltransferase V/MGAT5
Leu189-Leu741
Accession # Q09328
Specificity
Detects human N-Acetylglucosaminyltransferase V/MGAT5 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
MGAT5
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 2 ug/mL
Publications
Read Publications using
MAB5469 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for N-Acetylglucosaminyltransferase V/MGAT5 Antibody (706824)

  • alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A
  • Alpha-mannoside beta-1,6-N-acetylglucosaminyltransferase
  • EC 2.4.1.155
  • GGNT5
  • GlcNAc-T V
  • GNT-VGNT-VA
  • Mannoside acetylglucosaminyltransferase 5
  • mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase
  • MGAT5
  • N-Acetylglucosaminyltransferase V
  • N-acetylglucosaminyl-transferase V

Background

N-Acetylglucosaminyltransferase V (GnT-V), also known as mannosylglycoprotein N-acetyl-glucosaminyltransferase 5 (MGAT5), adds an N-acetylglucosamine to the alpha 1-6-linked core mannose of an N-linked oligosaccharide in the Golgi apparatus (1). This reaction is the committing step for the biosynthesis of beta 1-6GlcNAc-branched arm in N-glycans. The degree of N-glycan branching has been shown to regulate cell proliferation and differentiation (2). An increase in the GnT-V activity and its glycan products is also known to positively correlate with the progression of invasive malignancies (3, 4). For example, ectopic expression of GnT-V in epithelial cells results in morphological transformation and tumor growth in mice and overexpression in carcinoma cells has been shown to induce metastatic spread (3-5).

  1. Saito, H. et al. (1994) Biochem. Biophys. Res. Commun. 198:318.
  2. Lau, K.S. et al. (2007) Cell 198:123.
  3. Dennis, J.W. et al. (2002) Biochim. Biophys. Acta 1573:414.
  4. Granovsky, M. et al. (2008) Nat. Med. 7:1.
  5. Kim, Y.S. et al. (2008) Mol. Cell. Proteomics 7:1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for N-Acetylglucosaminyltransferase V/MGAT5 Antibody (MAB5469)(5)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: Western Blot.


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Western Blot
(3)
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Human
(3)
Mouse
(1)
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Showing Publications 1 - 5 of 5.
Publications using MAB5469 Applications Species
Tetsuya Hirata, Yoichiro Harada, Koichiro M. Hirosawa, Yuko Tokoro, Kenichi G.N. Suzuki, Yasuhiko Kizuka N-acetylglucosaminyltransferase-V (GnT-V)-enriched small extracellular vesicles mediate N-glycan remodeling in recipient cells iScience 1/20/2023 [PMID: 36590176]
Tetsuya Hirata, Misaki Takata, Yuko Tokoro, Miyako Nakano, Yasuhiko Kizuka Shedding of N-acetylglucosaminyltransferase-V is regulated by maturity of cellular N-glycan Communications Biology 8/1/2022 [PMID: 35915223]
RF Osuka, T Hirata, M Nagae, M Nakano, H Shibata, R Okamoto, Y Kizuka N-Acetylglucosaminyltransferase-V requires a specific non-catalytic luminal domain for its activity toward glycoprotein substrates The Journal of Biological Chemistry, 2022-01-30;0(0):101666. 2022-01-30 [PMID: 35104505] (Western Blot, Human) Western Blot Human
Priglinger C, Obermann J, Szober C, Merl-Pham J, Ohmayer U, Behler J, Gruhn F, Kreutzer T, Wertheimer C, Geerlof A, Priglinger S, Hauck S Epithelial-to-Mesenchymal Transition of RPE Cells In Vitro Confers Increased beta1,6-N-Glycosylation and Increased Susceptibility to Galectin-3 Binding. PLoS ONE, 2016-01-13;11(1):e0146887. 2016-01-13 [PMID: 26760037] (Western Blot, Human) Western Blot Human
Voss M, Kunzel U, Higel F, Kuhn P, Colombo A, Fukumori A, Haug-Kroper M, Klier B, Grammer G, Seidl A, Schroder B, Obst R, Steiner H, Lichtenthaler S, Haass C, Fluhrer R Shedding of glycan-modifying enzymes by signal peptide peptidase-like 3 (SPPL3) regulates cellular N-glycosylation. EMBO J, 2014-10-29;33(24):2890-905. 2014-10-29 [PMID: 25354954] (Western Blot, Human, Mouse) Western Blot Human, Mouse

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Bioinformatics

Gene Symbol MGAT5
Uniprot