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Myosin Phosphatase 2 Antibody

Images

 
Western Blot: Myosin Phosphatase 2 Antibody [NBP1-87751] - Analysis in control (vector only transfected HEK293T lysate) and PPP1R12B over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in ...read more
Orthogonal Strategies: Immunohistochemistry-Paraffin: Myosin Phosphatase 2 Antibody [NBP1-87751] - Staining in human heart muscle and pancreas tissues using anti-PPP1R12B antibody. Corresponding PPP1R12B RNA-seq ...read more
Immunohistochemistry-Paraffin: Myosin Phosphatase 2 Antibody [NBP1-87751] - Staining of human heart muscle shows high expression.
Immunohistochemistry-Paraffin: Myosin Phosphatase 2 Antibody [NBP1-87751] - Staining of human pancreas shows low expression as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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Myosin Phosphatase 2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: GTGINFWTKDEDETDGSEEVKETWHERLSRLESGGSNPTTSDSYGDRASARARREAREARLATLTSRVEEDSNRDYKKLYESALTENQKLKTKLQEAQLELADIKS
Predicted Species
Mouse (92%), Rat (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PPP1R12B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
Myosin Phosphatase 2 Protein (NBP1-87751PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Myosin Phosphatase 2 Antibody

  • MGC131980
  • MGC87886
  • Myosin phosphatase target subunit 2
  • myosin phosphatase, target subunit 2
  • Myosin phosphatase-targeting subunit 2
  • MYPT 2
  • MYPT2FLJ40942
  • protein phosphatase 1 regulatory subunit 12B
  • protein phosphatase 1, regulatory (inhibitor) subunit 12B

Background

PPP1R12B, also known as Protein phosphatase 1 regulatory subunit 12B, has 5 isoforms, a 982 amino acid isoform that is 110 kDa, a 386 amino acid isoform that is 43 kDa, a 224 amino acid isoform that is 26 kDa, a 208 amino acid isoform that is 24 kDa, and a 515 amino acid isoform that is 58 kDa; detected in skeletal muscle, fetal and adult heart, brain, placenta, kidney, spleen, thymus, pancreas and lung; with cytoplasmatic subcellular location; regulates myosin phosphatase activity and augments Ca(2+) sensitivity of the contractile apparatus. This protein is being studied for its involvement in breast cancer. The PPP1R12B protein has been shown to involve IL16, PPP1CB, PRKG1, HSPA8, MYL6, and MYL6B in RhoA pathway, PKA signaling, actin-based motility by Rho family GTPases, eIF2 pathway, CDK5 pathway, insulin pathway, G2/M transition, cell cycle, mitotic G2-G2/M phases, regulation of PLK1 activity at G2/M transition, cell cycle, mitotic, and vascular smooth muscle contraction pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PPP1R12B