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Rat Myosin light chain 3 ELISA Kit (Colorimetric)

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ELISA: Rat Myosin light chain 3 ELISA Kit (Colorimetric) [NBP2-75724] - Samples were spiked with high concentrations of Rat Myosin light chain 3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Rat Myosin light chain 3 ELISA Kit (Colorimetric) [NBP2-75724] - Samples were spiked with high concentrations of Rat Myosin light chain 3 and diluted with Reference Standard & Sample Diluent to produce samples ...read more
ELISA: Rat Myosin light chain 3 ELISA Kit (Colorimetric) [NBP2-75724] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.10 ng/mL

Order Details

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Rat Myosin light chain 3 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat CMLC-1 in samples. No significant cross-reactivity or interference between Rat CMLC-1 and analogues was observed.
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.10 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.03%
Intra-Assay
CV% < 5.32%
Spike Recovery
90-106%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
MYL3

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Rat Myosin light chain 3 ELISA Kit (Colorimetric)

  • Cardiac myosin light chain 1
  • CMH8Ventricular/slow twitch myosin alkali light chain
  • CMLC1
  • light polypeptide 3, alkali; ventricular, skeletal, slow
  • MLC1SBMyosin light chain 1, slow-twitch muscle B/ventricular isoform
  • MLC1V
  • myosin light chain 3
  • myosin, light chain 3, alkali; ventricular, skeletal, slow
  • VLC1

Background

Myosin light chain 3, or MYL3 for short, consists of a 195 amino acid isoform that is 22 kDa, and is involved in the regulation of Myosin, which is a protein that conducts ATP hydrolysis. Current research is being conducted on the relationship between Myosin light chain 3 and a multitude of diseases and disorders, including familial hypertrophic cardiomyopathy, congestive heart failure, restrictive cardiomyopathy, dilated cardiomyopathy, diabetes mellitus, and renal failure. Myosin light chain 3 has been linked to the RhoA pathway, as well as PKA signaling, growth cone motility, cell adhesion, cardiac muscle contraction, and cytoskeleton remodeling. The protein interacts with YWHAQ, MYH13, YWHAZ, MYH9, and MYH7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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  Rat Myosin light chain 3 ELISA Kit (Colorimetric) Manual
  PDF Datasheet
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Bioinformatics

Gene Symbol MYL3