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Myosin Light Chain 2 Antibody (3B2) [PE/Cy5.5]

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Product Details

Summary
Reactivity Hu, Mu, XpSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC
Clone
3B2
Clonality
Monoclonal
Host
Mouse
Conjugate
PE/Cy5.5

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-30249AF350
Alexa Fluor 405
NBP1-30249AF405
Alexa Fluor 488
NBP1-30249AF488
Alexa Fluor 532
NBP1-30249AF532
Alexa Fluor 594
NBP1-30249AF594
Alexa Fluor 647
NBP1-30249AF647
Alexa Fluor 700
NBP1-30249AF700
Alexa Fluor 750
NBP1-30249AF750
Allophycocyanin
NBP1-30249APC
Allophycocyanin/Cy7
NBP1-30249APCCY7
Biotin
NBP1-30249B
DyLight 350
NBP1-30249UV
DyLight 405
NBP1-30249V
DyLight 488
NBP1-30249G
DyLight 550
NBP1-30249R
DyLight 594
NBP1-30249DL594
DyLight 650
NBP1-30249C
DyLight 680
NBP1-30249FR
DyLight 755
NBP1-30249IR
FITC
NBP1-30249F
HRP
NBP1-30249H
Janelia Fluor 549
NBP1-30249JF549
Janelia Fluor 646
NBP1-30249JF646
PE
NBP1-30249PE
PE/Atto594
NBP1-30249PEATT594
PE/Cy7
NBP1-30249PECY7
PerCP
NBP1-30249PCP
Unconjugated
NBP1-30249
mFluor Violet 450 SE
NBP1-30249MFV450
mFluor Violet 500 SE
NBP1-30249MFV500
mFluor Violet 610 SE
NBP1-30249MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP1-30249
BSA Free
NBP1-30249
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Myosin Light Chain 2 Antibody (3B2) [PE/Cy5.5] Summary

Immunogen
Recombinant human Myosin Light Chain 2 (1-166aa) purified from E. coli (NP_000423).
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MYL2
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Reactivity Notes

Xenopus reactivity reported in scientific literature (PMID: 23727888). Mouse reactivity reported in scientific literature (PMID: 28430962).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Alternate Names for Myosin Light Chain 2 Antibody (3B2) [PE/Cy5.5]

  • cardiac ventricular myosin light chain 2
  • CMH10myosin light chain 2
  • DKFZp779C0562
  • MLC2
  • MLC-2
  • MLC-2v
  • myosin regulatory light chain 2, ventricular/cardiac muscle isoform
  • myosin, light chain 2, regulatory, cardiac, slow
  • myosin, light polypeptide 2, regulatory, cardiac, slow
  • regulatory light chain of myosin
  • RLC of myosin
  • slow cardiac myosin regulatory light chain 2

Background

MYL2 (myosin, light chain 2, regulatory, cardiac, slow), also known as MLC-2, MLC2v. Entrez protein NP_000423. MYL2 associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. It is an hexamer of two heavy chains and four light chains that is predominantly expressed in adult cardiac ventricle muscle. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol MYL2