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Myosin heavy chain 11 Antibody (JA03-35)

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Immunocytochemistry/Immunofluorescence: Myosin heavy chain 11 Antibody (JA03-35) [NBP2-66967] - Staining smooth muscle Myosin heavy chain 11 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/ Immunofluorescence: Myosin heavy chain 11 Antibody (JA03-35) [NBP2-66967] - Staining smooth muscle Myosin heavy chain 11 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were ...read more
Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody (JA03-35) [NBP2-66967] - Analysis of paraffin-embedded rat trachea tissue using anti-smooth muscle Myosin heavy chain 11 antibody. Counter stained with ...read more
Immunocytochemistry/ Immunofluorescence: Myosin heavy chain 11 Antibody (JA03-35) [NBP2-66967] - Staining smooth muscle Myosin heavy chain 11 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were ...read more
Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody (JA03-35) [NBP2-66967] - Analysis of paraffin-embedded human colon tissue using anti-smooth muscle Myosin heavy chain 11 antibody. Counter stained with ...read more
Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody (JA03-35) [NBP2-66967] - Analysis of paraffin-embedded human jejunum tissue using anti-smooth muscle Myosin heavy chain 11 antibody. Counter stained with ...read more
Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody (JA03-35) [NBP2-66967] - Analysis of paraffin-embedded human prostate tissue using anti-smooth muscle Myosin heavy chain 11 antibody. Counter stained with ...read more
Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody (JA03-35) [NBP2-66967] - Analysis of paraffin-embedded human stomach cancer tissue using anti-smooth muscle Myosin heavy chain 11 antibody. Counter stained ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
JA03-35
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

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Myosin heavy chain 11 Antibody (JA03-35) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Synthetic peptide within human smooth muscle Myosin heavy chain 11 aa 1935-1972/1972. (SwissProt: P35749 Human; SwissProt: Q63862 Rat)
Localization
Melanosome.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
MYH11
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500
Publications
Read Publications using
NBP2-66967 in the following applications:

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:34411694).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Myosin heavy chain 11 Antibody (JA03-35)

  • AAT4
  • FAA4
  • MYH11 myosin, heavy chain 11, smooth muscle
  • MYH11
  • SMHC
  • SMMHC
  • Smooth muscle myosin heavy chain

Background

The protein encoded by the MYH11 gene is a smooth muscle myosin belonging to the myosin heavy chain family. The gene product is a subunit of a hexameric protein that consists of two heavy chain subunits and two pairs of non-identical light chain subunits. MYH11 functions as a major contractile protein, converting chemical energy into mechanical energy through the hydrolysis of ATP. The gene encoding a human ortholog of rat NUDE1 is transcribed from the reverse strand of this gene, and its 3' end overlaps with that of the latter. The pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript that encodes a protein consisting of the first 165 residues from the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Alternative splicing generates isoforms that are differentially expressed, with ratios changing during muscle cell maturation. Alternatively spliced transcript variants encoding different isoforms have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Myosin heavy chain 11 Antibody (NBP2-66967)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: ICC/IF.


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Bioinformatics

Gene Symbol MYH11