| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ICC/IF |
| Clonality | Polyclonal |
| Host | Sheep |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Immunogen | E. coli-derived recombinant human CBFB Pro2-Glu165 Accession # Q13951 |
| Specificity | Detects human and mouse CBFB in Western blots and recombinant human CBFB in direct ELISAs. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human RUNX-1, -2, and -3 is observed. |
| Source | N/A |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Sheep |
| Gene | CBFB |
| Purity Statement | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
| Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Reconstitution Instructions | Sterile PBS to a final concentration of 0.2 mg/mL. |
CBFB (Core binding Factor beta; also PEA2 beta and PEBP2 beta ) is a widely-expressed 21-24 kDa member of the CBFB family of proteins. It forms dimeric transcriptional complexes with multiple molecules, including CBFB, RUNX1 and RUNX2. Although it does not bind DNA, it potentiates the binding of its partners to DNA. Human CBFB is 182 amino acids (aa) in length. It contains multiple alpha -helices and beta -strands. CBFB has at least two splice variants. One is approximately 23 kDa in size and possesses a 22 aa substitution for aa 166-182. A second is 16 kDa in size and contains a 22 aa substitution for aa 134-182. And a third shows a potential deletion of aa 56-94 coupled to the above 22 substitution for aa 166-182. CBFB is known to form a 68-70 kDa fusion protein with smooth muscle myosin heavy chain in AML. The CBFB contribution to the N-terminus of this fusion protein usually involves aa 1-165. Over aa 1-165, human CBFB shares 98% aa sequence identity with mouse CBFB.
Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry RUNX2/CBFA1 Antibody (232902) [Unconjugated]](https://images.novusbio.com/images2/RUNX2_MAB2006_Immunocytochemistry_13309.jpg)
![Immunohistochemistry RUNX2/CBFA1 Antibody (232902) [Unconjugated]](https://images.novusbio.com/images/mab2006_human-runx2-cbfa1-mab-clone-232902-812025555286.jpg)
![Western Blot c-Abl Antibody [Unconjugated]](https://images.novusbio.com/images2/c-Abl_AF5414_Western_Blot_5714.jpg)
![Simple Western c-Abl Antibody [Unconjugated]](https://images.novusbio.com/images2/cAbl_AF5414_Simple_Western_17046.jpg)
