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MyoD Antibody (474515) [Unconjugated]

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MyoD was detected in fixed C2C12 mouse myoblast cell line (Positive) and absent in RD cells (Negative) using Mouse Anti-Human MyoD Monoclonal Antibody (Catalog # MAB5966) at 25 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the nucleus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
MyoD was detected in fixed C2C12 mouse myoblast cell line (Positive) and absent in RD cells (Negative) using Mouse Anti-Human MyoD Monoclonal Antibody (Catalog # MAB5966) at 25 µg/ml for 3 hours at room temperature. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF
Clone
474515
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB5966-SP
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MyoD Antibody (474515) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human MyoD
Glu77-Gly215
Accession # P15172
Specificity
Detects human MyoD in direct ELISAs.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
MYOD1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
Publications
Read Publications using
MAB5966 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MyoD Antibody (474515) [Unconjugated]

  • bHLHc1
  • bHLHc1BHLHC1
  • Class C basic helix-loop-helix protein 1
  • MYF3
  • MYF3Myf-3
  • MyoD
  • MYOD1
  • MYODmyoblast determination protein 1
  • myogenic differentiation 1
  • Myogenic factor 3myf-3
  • PUM

Background

MyoD (myoblast determination protein 1), also called Myf-3 (myogenic factor 3) is an ~44 kDa nuclear protein in the MyoD family of muscle-specific bHLH transcription factors. MyoD family members heterodimerize with E proteins and cooperate with MEF2 family transcription factors to regulate expression of skeletal muscle-specific genes. MyoD is essential for skeletal muscle differentiation. Acetylation at lysines 99, 102 and 104 further regulates its activity. Human MyoD shares 96% amino acid identity with mouse and rat MyoD over the sequence used as the immunogen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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(474515) [Unconjugate
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MyoD Antibody (474515) [Unconjugated]
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Publications for MyoD Antibody (MAB5966)(2)

We have publications tested in 2 confirmed species: Human, Xenograft.

We have publications tested in 2 applications: Flow Cytometry, IHC-P.


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Flow Cytometry
(1)
IHC-P
(1)
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(1)
Xenograft
(1)
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FAQs for MyoD Antibody (MAB5966) (0)

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol MYOD1
Uniprot