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MYLK2 Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: MYLK2 Antibody [NBP2-32489] - Staining in human skeletal muscle and heart muscle tissues using anti-MYLK2 antibody. Corresponding MYLK2 RNA-seq data are ...read more
Western Blot: MYLK2 Antibody [NBP2-32489] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: MYLK2 Antibody [NBP2-32489] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum.
Immunohistochemistry-Paraffin: MYLK2 Antibody [NBP2-32489] - Staining of human heart muscle shows low expression as expected.
Immunohistochemistry-Paraffin: MYLK2 Antibody [NBP2-32489] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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MYLK2 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: IEFQAVPSEKSEVGQALCLTAREEDCFQILDDCPPPPAPFPHRMVELRTGNVSSEFSMNSKEALGGGKFGAVCTCMEKAT
Predicted Species
Mouse (91%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MYLK2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
MYLK2 Protein (NBP2-32489PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (89%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for MYLK2 Antibody

  • EC 2.7.11
  • EC 2.7.11.18
  • KMLC
  • MLCK2
  • MLCK2MLCK
  • MYLK2
  • myosin light chain kinase 2
  • myosin light chain kinase 2, skeletal/cardiac muscle
  • skeletal muscle
  • skMLCK

Background

MYLK2 encodes a myosin light chain kinase, a calcium/calmodulin dependent enzyme, that is exclusively expressed in adult skeletal muscle.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MYLK2 Antibody (NBP2-32489) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MYLK2