![Western Blot: MVP Antibody (2H3-1A6) [H00009961-M01] - Western blot of several proteins that were increased or decreased in DBA patients including dysferlin (DYSF), gamma-adducin (ADD3), major vault protein (MVP), serine palmitoyltransferase 1 and 2 (SPTLC1 and SPTLC2), HLA-ABC, and intercellular adhesion molecule 4 (ICAM4) with b-actin (ACTB) used as a loading control. Six individual control samples that were included in the pooled controls for proteomic analysis were used for comparison to individual DBA patient samples. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0085504), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MVP-Antibody-2H3-1A6-Western-Blot-H00009961-M01-img0011.jpg "Western Blot: MVP Antibody (2H3-1A6) [H00009961-M01] - Western blot of several proteins that were increased or decreased in DBA patients including dysferlin (DYSF), gamma-adducin (ADD3), major vault protein (MVP), serine palmitoyltransferase 1 and 2 (SPTLC1 and SPTLC2), HLA-ABC, and intercellular adhesion molecule 4 (ICAM4) with b-actin (ACTB) used as a loading control. Six individual control samples that were included in the pooled controls for proteomic analysis were used for comparison to individual DBA patient samples. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0085504), licensed under a CC-BY license.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clone | 2H3-1A6 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | MVP (AAH15623, 1 a.a. ~ 893 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MATEEFIIRIPPYHYIHVLDQNSNVSRVEVGPKTYIRQDNERVLFAPMRMVTVPPRHYCTVANPVSRDAQGLVLFDVTGQVRLRHADLEIRLAQDPFPLYPGEVLEKDITPLQVVLPNTALHLKALLDFEDKDGDKVVAGDEWLFEGPGTYIPRKEVEVVEIIQATIIRQNQALRLRARKECWDRDGKERVTGEEWLVTTVGAYLPAVFEEVLDLVDAVILTEKTALHLRARRNFRDFRGVSRRTGEEWLVTVQDTEAHVPDVHEEVLGVVPITTLGPHNYCVILDPVGPDGKNQLGQKRVVKGEKSFFLQPGEQLEQGIQDVYVLSEQQGLLLRALQPLEEGEDEEKVSHQAGDHWLIRGPLEYVPSAKVEVVEERQAIPLDENEGIYVQDVKTGKVRAVIGSTYMLTQDEVLWEKELPPGVEELLNKGQDPLADRGEKDTAKSLQPLAPRNKTRVVSYRVPHNAAVQVYDYREKRARVVFGPELVSLGPEEQFTVLSLSAGRPKRPHARRALCLLLGPDFFTDVITIETADHARLQLQLAYNWHFEVNDRKDPQETAKLFSVPDFVGDACKAIASRVRGAVASVTFDDFHKNSARIIRTAVFGFETSEAKGPDGMALPRPRDQAVFPQNGLVVSSVDVQSVEPVDQRTRDALQRSVQLAIEITTNSQEAAAKHEAQRLEQEARGRLERQKILDQSEAEKARKELLELEALSMAVESTGTAKAEAESRAEAARIEGEGSVLQAKLKAQALAIETEAELQRVQKVRELELVYARAQLELEVSKAQQLAEVEVKKFKQMTEAIGPSTIRDLAVAGPEMQVKLLQSLGLKSTLITDGSTPINLFNTAFGLLGMGPEGQPLGRRVASGPSPGEGISPQSAQAPQAPGDNHVVPVLR |
| Localization | Cytoplasmic, 5% are nucleus associated and localize to the nuclear pore complexes |
| Specificity | MVP - major vault protein |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | MVP |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Application Notes | Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA. |
|
| Publications |
|
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for MVP Antibody (H00009961-M01)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: MVP Antibody (2H3-1A6) [H00009961-M01] - Analysis of monoclonal antibody to MVP on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/MVP-Antibody-2H3-1A6-Immunocytochemistry-Immunofluorescence-H00009961-M01-img0006.jpg "Immunocytochemistry/Immunofluorescence: MVP Antibody (2H3-1A6) [H00009961-M01] - Analysis of monoclonal antibody to MVP on HeLa cell. Antibody concentration 10 ug/ml.")
![Immunohistochemistry-Paraffin: MVP Antibody (2H3-1A6) [H00009961-M01] - Analysis of monoclonal antibody to MVP on formalin-fixed paraffin-embedded human lung, adenosquamous cell carcinoma. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/MVP-Antibody-2H3-1A6-Immunohistochemistry-Paraffin-H00009961-M01-img0008.jpg "Immunohistochemistry-Paraffin: MVP Antibody (2H3-1A6) [H00009961-M01] - Analysis of monoclonal antibody to MVP on formalin-fixed paraffin-embedded human lung, adenosquamous cell carcinoma. Antibody concentration 3 ug/ml.")
![Western Blot: MVP Antibody (2H3-1A6) [H00009961-M01] - MVP monoclonal antibody (M01), clone 2H3-1A6 Analysis of MVP expression in Hela S3 NE.](http://images.novusbio.com/fullsize/MVP-Antibody-2H3-1A6-Western-Blot-H00009961-M01-img0010.jpg "Western Blot: MVP Antibody (2H3-1A6) [H00009961-M01] - MVP monoclonal antibody (M01), clone 2H3-1A6 Analysis of MVP expression in Hela S3 NE.")
![ELISA: MVP Antibody (2H3-1A6) [H00009961-M01] - Detection limit for recombinant GST tagged MVP is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/MVP-Antibody-2H3-1A6-Sandwich-ELISA-H00009961-M01-img0009.jpg "ELISA: MVP Antibody (2H3-1A6) [H00009961-M01] - Detection limit for recombinant GST tagged MVP is approximately 1ng/ml as a capture antibody.")
![Western Blot: MVP Antibody (2H3-1A6) [H00009961-M01] - Immunoblot validation & characterization of dysferlin.A) Western blot of several proteins that were increased or decreased in DBA patients including dysferlin (DYSF), gamma-adducin (ADD3), major vault protein (MVP), serine palmitoyltransferase 1 & 2 (SPTLC1 & SPTLC2), HLA-ABC, & intercellular adhesion molecule 4 (ICAM4) with beta -actin (ACTB) used as a loading control. Six individual control samples that were included in the pooled controls for proteomic analysis were used for comparison to individual DBA patient samples. B) Confocal images of intact RBCs from DBA patient D1 & healthy control C2 showing localization of dysferlin (green) at the membrane where spectrin (red) is localized. Dysferlin fluorescence signal in the membrane of DBA RBCs is present in punctuate patterns & is evident above the autofluorescent background seen in the cytoplasm as highlighted in the zoomed images on the right panel. C) Western blot showing the enrichment of dysferlin in the RBC ghosts (WG) & the inside-out vesicles (IOV) but not in the RBC membrane cytoskeletal extract (CE) of DBA patients D1 & D2. D) Distribution of dysferlin spectral counts versus gel slice number for DBA samples D1 & D2. Higher abundance of dysferlin in D1 with high molecular weight dysferlin crosslinks & low mass protein fragments correlate with immunoblots shown in panel A. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24454878), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00009961-m01_mouse-monoclonal-mvp-antibody-2h3-1a6-310202415485144.jpg "Western Blot: MVP Antibody (2H3-1A6) [H00009961-M01] - Immunoblot validation & characterization of dysferlin.A) Western blot of several proteins that were increased or decreased in DBA patients including dysferlin (DYSF), gamma-adducin (ADD3), major vault protein (MVP), serine palmitoyltransferase 1 & 2 (SPTLC1 & SPTLC2), HLA-ABC, & intercellular adhesion molecule 4 (ICAM4) with beta -actin (ACTB) used as a loading control. Six individual control samples that were included in the pooled controls for proteomic analysis were used for comparison to individual DBA patient samples. B) Confocal images of intact RBCs from DBA patient D1 & healthy control C2 showing localization of dysferlin (green) at the membrane where spectrin (red) is localized. Dysferlin fluorescence signal in the membrane of DBA RBCs is present in punctuate patterns & is evident above the autofluorescent background seen in the cytoplasm as highlighted in the zoomed images on the right panel. C) Western blot showing the enrichment of dysferlin in the RBC ghosts (WG) & the inside-out vesicles (IOV) but not in the RBC membrane cytoskeletal extract (CE) of DBA patients D1 & D2. D) Distribution of dysferlin spectral counts versus gel slice number for DBA samples D1 & D2. Higher abundance of dysferlin in D1 with high molecular weight dysferlin crosslinks & low mass protein fragments correlate with immunoblots shown in panel A. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24454878), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunohistochemistry LDLR Antibody [Unconjugated]](https://images.novusbio.com/images2/LDL_R_AF2255_Immunohistochemistry_7149.jpg)
![Western Blot LDLR Antibody [Unconjugated]](https://images.novusbio.com/images2/LDLR_AF2255_Western_Blot_23731.jpg)
![Western Blot LDLR Antibody [Unconjugated]](https://images.novusbio.com/images/af2255_mouse-ldl-r-affinity-purified-polyclonal-ab-4120241049445.jpg)
![N/A t-Plasminogen Activator/tPA [HRP]](https://images.novusbio.com/images2/DATA_tPlasminogen_Activator_DTPA00_ELISA_854.jpg)
![N/A t-Plasminogen Activator/tPA [HRP]](https://images.novusbio.com/images2/tPlasminogen_Activator_DTPA00_ELISA_510.jpg)
![Enzyme Activity u-Plasminogen Activator/Urokinase [Unconjugated]](https://images.novusbio.com/images2/uPlasminogen_Activator_1310SE_1837.jpg)
![Immunohistochemistry Lipoprotein Lipase/LPL Antibody [Unconjugated]](https://images.novusbio.com/images2/Lipoprotein_Lipase_AF7197_Immunohistochemistry_21186.jpg)
![Western Blot Lipoprotein Lipase/LPL Antibody [Unconjugated]](https://images.novusbio.com/images2/Lipoprotein_Lipase_AF7197_Western_Blot_11656.jpg)
![Immunocytochemistry Lipoprotein Lipase/LPL Antibody [Unconjugated]](https://images.novusbio.com/images2/Lipoprotein_Lipase_AF7197_Immunocytochemistry_11911.jpg)
![N/A Kallikrein 3/PSA [HRP]](https://images.novusbio.com/images2/DATA_Kallikrein_3_DKK300_ELISA_729.jpg)
![N/A Kallikrein 3/PSA [HRP]](https://images.novusbio.com/images2/DATA_Kallikrein_3_DKK300_ELISA_730.jpg)
![N/A Kallikrein 3/PSA [HRP]](https://images.novusbio.com/images2/Kallikrein_3_DKK300_ELISA_124.jpg)
