Novus Biologicals products are now on bio-techne.com

MDMX Antibody

Images

 
Western Blot: MDMX Antibody [NB100-556] - Detection of human HdmX/MDM4 by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, HEK293T, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity ...read more
Western Blot: MDMX Antibody [NB100-556] - Analysis using the Biotin conjugate of NB100-556. Detection of Jurkat (50, 15, and 5 ug). Antibody used at 1.0 ug/ml. Detection: Streptavidin-HRP and chemiluminescence with an ...read more
Independent Antibodies: Immunoprecipitation: MDMX Antibody [NB100-556] - Detection of human HdmX/MDM4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
 

Independent Antibodies

       

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

MDMX Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 125 and 175 of human ortholog of mouse double minute 4 using the numbering given in Swiss-Prot entry O15151 (GeneID 4194).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MDM4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoprecipitation 2 -10 ug/mg lysate
  • Western Blot 1:1000-1:5000
Control
CC2D1A Overexpression Lysate
Publications
Read Publications using NB100-556.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MDMX Antibody

  • DKFZp781B1423
  • double minute 4 homolog
  • Double minute 4 protein
  • double minute 4, human homolog of; p53-binding protein
  • HDMX
  • Mdm2-like p53-binding protein
  • Mdm4 p53 binding protein homolog (mouse)
  • MDM4 Regulator of P53
  • MDM4
  • Mdm4, transformed 3T3 cell double minute 4, p53 binding protein (mouse)
  • Mdm4, transformed 3T3 cell double minute 4, p53 binding protein
  • MDM4-related protein 1
  • MDMX
  • MDMXMGC132766
  • mouse double minute 4, human homolog of; p53-binding protein
  • MRP1
  • p53-binding protein Mdm4
  • protein Mdm4
  • Protein Mdmx

Background

HdmX/MDM-4 is the human homolog of the murine double minute 2 (Mdm2) protein. It is a critical negative regulator of the p53 tumor suppressor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB400-156
Species: Hu, Mu, Rt
Applications: CyTOF-ready, Dual ISH-IHC, ELISA, Flow, ICC/IF, IHC, IP, Simple Western, WB
NBP2-67667
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
NBP1-69023
Species: Mu
Applications: WB
NBP2-22124
Species: Hu, Mu, Pm
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NB200-103
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NBP1-76670
Species: Hu, Rt
Applications: ELISA, IHC, IHC-P, WB
NBP2-13415
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB100-2736
Species: Hu, Mu, Rt
Applications: IHC, IHC-Fr, IHC-P, IP, WB
NBP2-38160
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-57299
Species: Hu
Applications: IHC, IHC-P, WB
NBP2-37923
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-82820
Species: Hu
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
NBP3-41339
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, WB
NBP2-46467
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NB100-1471
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, PEP-ELISA, WB
AF6278
Species: Hu, Mu
Applications: WB
NB100-64808
Species: Hu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IP, WB
NBP3-14454
Species: Hu
Applications: IHC, IHC-P
NBP2-79854
Species: Hu
Applications: CyTOF-ready, Flow, ICC/IF, IHC, IHC-P, PA, WB
NB100-556
Species: Hu
Applications: WB, IP

Publications for MDMX Antibody (NB100-556)(33)


Showing Publications 1 - 10 of 33. Show All 33 Publications.
Publications using NB100-556 Applications Species
Morales-Martínez, M;Andón-García, D;Patiño-Santiago, KA;Parga-Ortega, JM;Hernández-Hernández, A;Aquino-Jarquin, G;Patino-Lopez, G; Identification of potential new T cell activation molecules: a Bioinformatic Approach Scientific reports 2024-09-27 [PMID: 39333573]
McEvoy J, Ulyanov A, Brennan R et al. Analysis of MDM2 and MDM4 single nucleotide polymorphisms, mRNA splicing and protein expression in retinoblastoma. PLoS One 2012-01-01 [PMID: 22916154]
Mandke P, Wyatt N, Fraser J et al. MicroRNA-34a modulates MDM4 expression via a target site in the open reading frame. PLoS One 2012-01-01 [PMID: 22870278]
Leventaki V, Rodic V, Tripp SR et al. TP53 pathway analysis in paediatric Burkitt lymphoma reveals increased MDM4 expression as the only TP53 pathway abnormality detected in a subset of cases. Br J Haematol 2012-09-01 [PMID: 22845047]
Henze J, Muhlenberg T, Simon S et al. p53 modulation as a therapeutic strategy in gastrointestinal stromal tumors. PLoS One 2012-01-01 [PMID: 22662219]
Lee JH, Jin Y, He G et al. Hypoxia activates tumor suppressor p53 by inducing ATR-Chk1 kinase cascade-mediated phosphorylation and consequent 14-3-3gammainactivation of MDMX protein. J Biol Chem 2012-06-01 [PMID: 22556425]
Biderman L, Poyurovsky MV, Assia Y et al. MdmX is required for p53 interaction with and full induction of the Mdm2 promoter after cellular stress. Mol Cell Biol 2012-04-01 [PMID: 22290440]
Wang X, Wang J, Jiang X et al. MdmX protein is essential for Mdm2 protein-mediated p53 polyubiquitination. J Biol Chem 2011-07-01 [PMID: 21572037]
Li D, Marchenko ND, Schulz R et al. Functional inactivation of endogenous MDM2 and CHIP by HSP90 causes aberrant stabilization of mutant p53 in human cancer cells. Mol Cancer Res 2011-05-01 [PMID: 21478269]
Anderson VE, Walton MI, Eve PD et al. CCT241533 is a potent and selective inhibitor of CHK2 that potentiates the cytotoxicity of PARP inhibitors. Cancer Res 2011-01-01 [PMID: 21239475]
Show All 33 Publications.

Reviews for MDMX Antibody (NB100-556) (0)

There are no reviews for MDMX Antibody (NB100-556). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for MDMX Antibody (NB100-556) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional MDMX Products

Research Areas for MDMX Antibody (NB100-556)

Find related products by research area.

Blogs on MDMX

There are no specific blogs for MDMX, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our MDMX Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol MDM4
Entrez
Uniprot