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Musashi-2 Antibody (OTI2F10)

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Western Blot: Musashi-2 Antibody (OTI2F10) [NBP2-45837] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MSI2 .
Immunohistochemistry: Musashi-2 Antibody (OTI2F10) [NBP2-45837] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: Musashi-2 Antibody (OTI2F10) [NBP2-45837] - Analysis of extracts (35ug) from 9 different cell lines by using MSI2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; ...read more
Immunohistochemistry: Musashi-2 Antibody (OTI2F10) [NBP2-45837] - Analysis of Adenocarcinoma of Human ovary tissu. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: Musashi-2 Antibody (OTI2F10) [NBP2-45837] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: Musashi-2 Antibody (OTI2F10) [NBP2-45837] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: Musashi-2 Antibody (OTI2F10) [NBP2-45837] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunofluorescence: Musashi-2 Antibody (OTI2F10) [NBP2-45837] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY Musashi-2.
Immunofluorescence: Musashi-2 Antibody (OTI2F10) [NBP2-45837] - Analysis of HeLa cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI2F10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

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Musashi-2 Antibody (OTI2F10) Summary

Immunogen
Full length human recombinant protein of human MSI2(NP_620412) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MSI2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:200 - 1:1000
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Musashi-2 Antibody (OTI2F10)

  • FLJ36569
  • MGC3245
  • MSI2
  • MSI2H
  • musashi homolog 2 (Drosophila)
  • Musashi2
  • Musashi-2
  • RNA-binding protein Musashi homolog 2

Background

Musashi 2 encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Two transcript variants encoding distinct isoforms have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Musashi-2 Antibody (NBP2-45837) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MSI2