| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52978SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72836 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76575-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3255-SP | |||
![Western Blot: Musashi-2 Antibody [NBP2-52978] - Total protein from HeLa, Neuro2A and SH-SY5Y cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-MS12 in 1% milk, and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Musashi-2-Antibody-Western-Blot-NBP2-52978-img0003.jpg "Western Blot: Musashi-2 Antibody [NBP2-52978] - Total protein from HeLa, Neuro2A and SH-SY5Y cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-MS12 in 1% milk, and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Musashi-2 Antibody [NBP2-52978] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-MSI2 at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Musashi-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-52978-img0001.jpg)
![Immunohistochemistry-Paraffin: Musashi-2 Antibody [NBP2-52978] - IHC analysis of a formalin fixed paraffin embedded tissue section of murine pancreas using rabbit anti-Musashi-2 antibody at 1:400 dilution. The signal was developed using HRP-DAB based detection method and the counter-staining was performed with hematoxylin. The antibody generated a weaker signal in the exocrine glandular cells (acini) with very strong staining in the Islets of Langerhans, especially in the alpha cells.](http://images.novusbio.com/fullsize/Musashi-2-Antibody-Immunohistochemistry-Paraffin-NBP2-52978-img0002.jpg)
![Western Blot: Musashi 2 Antibody [NBP2-19443] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Musashi-2-Antibody-Western-Blot-NBP2-19443-img0002.jpg "Western Blot: Musashi 2 Antibody [NBP2-19443] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: Musashi-2 Antibody [NBP2-19443] - Rat brain. Musashi 2 stained by Musashi 2 antibody [N1C1] diluted at 1:500.Antigen Retrieval: Trilogy™M (EDTA based) buffer, 15min.](http://images.novusbio.com/fullsize/Musashi-2-Antibody-Immunohistochemistry-Paraffin-NBP2-19443-img0009.jpg)
![Western Blot: Musashi 2 Antibody [NBP2-19443] - Sample (30 ug of whole cell lysate) A: MCF-7 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Musashi-2-Antibody-Western-Blot-NBP2-19443-img0001.jpg)
![Immunohistochemistry-Frozen: Musashi-2 Antibody [NBP2-19443] - Frozen sectioned adult mouse retina. Green: Musashi 2 protein stained by Musashi 2 antibody [N1C1] diluted at 1:250. Red: Protein kinase C alpha staining. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Musashi-2-Antibody-Immunohistochemistry-Frozen-NBP2-19443-img0005.jpg)
![Immunohistochemistry-Paraffin: Musashi-2 Antibody [NBP2-19443] - Rat fore brain. Musashi 2 antibody [N1C1] dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Musashi-2-Antibody-Immunohistochemistry-Paraffin-NBP2-19443-img0007.jpg)
![Immunohistochemistry-Paraffin: Musashi-2 Antibody [NBP2-19443] - Mouse hind brain. Musashi 2 antibody [N1C1] dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Musashi-2-Antibody-Immunohistochemistry-Paraffin-NBP2-19443-img0008.jpg)
![Western Blot: Musashi-2 Antibody (OTI2F10) [NBP2-45837] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MSI2 .](http://images.novusbio.com/fullsize/Musashi-2-Antibody-OTI2F10-Western-Blot-NBP2-45837-img0008.jpg "Western Blot: Musashi-2 Antibody (OTI2F10) [NBP2-45837] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MSI2 .")
![Immunohistochemistry: Musashi-2 Antibody (OTI2F10) [NBP2-45837] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Musashi-2-Antibody-OTI2F10-Immunohistochemistry-NBP2-45837-img0007.jpg)
![Western Blot: Musashi-2 Antibody (OTI2F10) [NBP2-45837] - Analysis of extracts (35ug) from 9 different cell lines by using MSI2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)](http://images.novusbio.com/fullsize/Musashi-2-Antibody-OTI2F10-Western-Blot-NBP2-45837-img0009.jpg)
![Immunohistochemistry: Musashi-2 Antibody (OTI2F10) [NBP2-45837] - Analysis of Adenocarcinoma of Human ovary tissu. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Musashi-2-Antibody-OTI2F10-Immunohistochemistry-NBP2-45837-img0003.jpg)
![Immunohistochemistry: Musashi-2 Antibody (OTI2F10) [NBP2-45837] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Musashi-2-Antibody-OTI2F10-Immunohistochemistry-NBP2-45837-img0004.jpg)
![Immunohistochemistry: Musashi-2 Antibody (OTI2F10) [NBP2-45837] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Musashi-2-Antibody-OTI2F10-Immunohistochemistry-NBP2-45837-img0005.jpg)
![Immunohistochemistry: Musashi-2 Antibody (OTI2F10) [NBP2-45837] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Musashi-2-Antibody-OTI2F10-Immunohistochemistry-NBP2-45837-img0006.jpg)
![Immunofluorescence: Musashi-2 Antibody (OTI2F10) [NBP2-45837] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY Musashi-2.](http://images.novusbio.com/fullsize/Musashi-2-Antibody-OTI2F10-Immunofluorescence-NBP2-45837-img0001.jpg)
![Immunofluorescence: Musashi-2 Antibody (OTI2F10) [NBP2-45837] - Analysis of HeLa cells.](http://images.novusbio.com/fullsize/Musashi-2-Antibody-OTI2F10-Immunofluorescence-NBP2-45837-img0002.jpg)
![Western Blot: Musashi-2 Antibody [NBP1-76575] - Analysis of MSI2 in EL4 cell lysate with MSI2 antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/Musashi-2-Antibody-Western-Blot-NBP1-76575-img0004.jpg "Western Blot: Musashi-2 Antibody [NBP1-76575] - Analysis of MSI2 in EL4 cell lysate with MSI2 antibody at 1 ug/mL.")
![Immunocytochemistry/Immunofluorescence: Musashi-2 Antibody [NBP1-76575] - EL4 cells with MSI2 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/Musashi-2-Antibody-Immunocytochemistry-NBP1-76575-img0002.jpg)
![Immunohistochemistry-Paraffin: Musashi-2 Antibody [NBP1-76575] - Staining of MSI2 in mouse brain tissue with MSI2 Antibodyat 5 ug/mL.](http://images.novusbio.com/fullsize/Musashi-2-Antibody-Immunohistochemistry-NBP1-76575-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Musashi-2 Antibody [NBP1-76575] - EL4 cells with MSI2 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/Musashi-2-Antibody-Immunofluorescence-NBP1-76575-img0003.jpg)
![Immunohistochemistry: Musashi-2 Antibody [NBP1-76575] - Staining of MSI2 in mouse brain tissue with MSI2 Antibodyat 20 ug/mL.](http://images.novusbio.com/fullsize/Musashi-2-Antibody-Immunofluorescence-NBP1-76575-img0005.jpg)
![Western Blot: Musashi-2 Antibody (PSH01-17) [NBP3-32608] - Western blot analysis of Musashi-2 on different lysates with Rabbit anti-Musashi-2 antibody (NBP3-32608) at 1/1,000 dilution. Lane 1: PC-12 cell lysate (20 ug/Lane) Lane 2: K-562 cell lysate (20 ug/Lane) Lane 3: Human placenta tissue lysate (40 ug/Lane) Lane 4: Mouse brain tissue lysate (40 ug/Lane) Lane 5: Rat brain tissue lysate (40 ug/Lane) Lane 6: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 35 kDa Observed band size: 35/37 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32608) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32608_rabbit-musashi-2-mab-psh01-17-278202416285210.jpg "Western Blot: Musashi-2 Antibody (PSH01-17) [NBP3-32608] - Western blot analysis of Musashi-2 on different lysates with Rabbit anti-Musashi-2 antibody (NBP3-32608) at 1/1,000 dilution. Lane 1: PC-12 cell lysate (20 ug/Lane) Lane 2: K-562 cell lysate (20 ug/Lane) Lane 3: Human placenta tissue lysate (40 ug/Lane) Lane 4: Mouse brain tissue lysate (40 ug/Lane) Lane 5: Rat brain tissue lysate (40 ug/Lane) Lane 6: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 35 kDa Observed band size: 35/37 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32608) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Musashi-2 Antibody (PSH01-17) [NBP3-32608] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Musashi-2 antibody (NBP3-32608) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32608) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32608_rabbit-musashi-2-mab-psh01-17-298202414262658.jpg)
![Immunocytochemistry/ Immunofluorescence: Musashi-2 Antibody (PSH01-17) [NBP3-32608] - Immunocytochemistry analysis of HeLa cells labeling Musashi-2 with Rabbit anti-Musashi-2 antibody (NBP3-32608) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Musashi-2 antibody (NBP3-32608) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32608_rabbit-musashi-2-mab-psh01-17-2920241192390.jpg)
![Western Blot: Musashi-2 Antibody (RM422) [NBP3-09175] - Western Blot of mouse brain tissue lysate using NBP3-09175 at a 1:2000 dilution.](http://images.novusbio.com/fullsize/Musashi-2-Antibody-RM422-Western-Blot-NBP3-09175-img0003.jpg "Western Blot: Musashi-2 Antibody (RM422) [NBP3-09175] - Western Blot of mouse brain tissue lysate using NBP3-09175 at a 1:2000 dilution.")
![Immunohistochemistry-Paraffin: Musashi-2 Antibody (RM422) [NBP3-09175] - Immunohistochemical staining of formalin fixed and paraffin embedded human esophageal squamous carcinoma tissue section using NBP3-09175 at a 1:100 dilution.](http://images.novusbio.com/fullsize/Musashi-2-Antibody-RM422-Immunohistochemistry-Paraffin-NBP3-09175-img0002.jpg)
![Immunohistochemistry-Paraffin: Musashi-2 Antibody (RM422) [NBP3-09175] - Immunohistochemical staining of formalin fixed and paraffin embedded human breast ductal carcinoma tissue section using NBP3-09175 at a 1:400 dilution.](http://images.novusbio.com/fullsize/Musashi-2-Antibody-RM422-Immunohistochemistry-Paraffin-NBP3-09175-img0001.jpg)
![Western Blot: Musashi-2 Antibody (7L4E9) [NBP3-15935] - Western blot analysis of extracts of various cell lines, using Musashi-2 (Musashi-2) Rabbit mAb (NBP3-15935) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/Musashi-2-Antibody-7L4E9-Western-Blot-NBP3-15935-img0003.jpg "Western Blot: Musashi-2 Antibody (7L4E9) [NBP3-15935] - Western blot analysis of extracts of various cell lines, using Musashi-2 (Musashi-2) Rabbit mAb (NBP3-15935) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Immunohistochemistry-Paraffin: Musashi-2 Antibody (7L4E9) [NBP3-15935] - Immunohistochemistry of paraffin-embedded mouse brain using Musashi-2 (Musashi-2) Rabbit mAb (NBP3-15935) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Musashi-2-Antibody-7L4E9-Immunohistochemistry-Paraffin-NBP3-15935-img0002.jpg)
![Immunohistochemistry-Paraffin: Musashi-2 Antibody (7L4E9) [NBP3-15935] - Immunohistochemistry of paraffin-embedded rat brain using Musashi-2 (Musashi-2) Rabbit mAb (NBP3-15935) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Musashi-2-Antibody-7L4E9-Immunohistochemistry-Paraffin-NBP3-15935-img0001.jpg)
![Western Blot: Musashi 2 Overexpression Lysate (Adult Normal) [NBL1-13329] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Musashi 2.](http://images.novusbio.com/fullsize/Musashi-2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13329-img0002.jpg "Western Blot: Musashi 2 Overexpression Lysate (Adult Normal) [NBL1-13329] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Musashi 2.")
![SDS-Page: Recombinant Human Musashi-2 Protein [NBP2-23252] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain. Molecular Weight:37.6 kDa (351aa), confirmed by MALDI-TOF](http://images.novusbio.com/fullsize/Recombinant-Human-Musashi-2-Protein-SDS-Page-NBP2-23252-img0001.jpg "SDS-Page: Recombinant Human Musashi-2 Protein [NBP2-23252] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain. Molecular Weight:37.6 kDa (351aa), confirmed by MALDI-TOF")