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MTA2 Antibody

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Immunohistochemistry-Paraffin: MTA2 Antibody [NBP1-82615] - Analysis in human skin and liver tissues. Corresponding MTA2 RNA-seq data are presented for the same tissues.
Orthogonal Strategies: Immunohistochemistry-Paraffin: MTA2 Antibody [NBP1-82615] - Analysis in human skin and liver tissues. Corresponding MTA2 RNA-seq data are presented for the same tissues.
Western Blot: MTA2 Antibody [NBP1-82615] - Analysis of mouse cell line NIH-3T3 and rat cell line NBT-II
Immunocytochemistry/ Immunofluorescence: MTA2 Antibody [NBP1-82615] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: MTA2 Antibody [NBP1-82615] - Staining of human tonsil shows strong nuclear positivity in germinal center cells.
Western Blot: MTA2 Antibody [NBP1-82615] - Analysis in human cell line MOLT-4.
Immunohistochemistry-Paraffin: MTA2 Antibody [NBP1-82615] - Staining of human liver shows very weak nuclear positivity in hepatocytes.
Immunohistochemistry-Paraffin: MTA2 Antibody [NBP1-82615] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: MTA2 Antibody [NBP1-82615] - Staining of human skin shows strong nuclear positivity in squamous epithelial cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

  

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MTA2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: ECSIRLPKAAKTPLKIHPLVRLPLATIVKDLVAQAPLKPKTPRGTKTPINRNQLSQNRGLGGIMVKRAYETMAGAGVPFSANGRPLASGIRSSSQPAAKRQKLNPADAPNPVVFVATKDTRALRKALTHLEMRRAARRPNLPLKVKPT
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MTA2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
MTA2 Protein (NBP1-82615PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for MTA2 Antibody

  • DKFZp686F2281
  • metastasis associated 1 family, member 2
  • metastasis -associated gene 1-like 1
  • metastasis associated gene family, member 2
  • Metastasis-associated 1-like 1
  • metastasis-associated protein 2
  • metastasis-associated protein MTA2
  • MTA1-L1 protein
  • MTA1L1MTA1-L1
  • p53 target protein in deacetylase complex
  • PID

Background

Official Gene Symbol: MTA2 Gen Bank Accession Number: NP_004730 Gene ID: 9219 (Human) Gene Map Locus: 11q12-q13.1 (human) MTA2, a homologue of human MTA1 and MTA3, is a member of highly conserved MTA gene family. It is a component of NuRD, an ATP-dependent nucleosome remodeling and histone deacetylase complex. MTA2 is a nuclear protein that interacts with HDAC1 and HDAC2 and has a functional role in chromatin remodeling and deacetylase activity. MTA2 specifically interacts with p53 and represses p53-dependant transcriptional activation, thereby regulating p53-mediated cell growth arrest and apoptosis. It also inhibits the transcriptional activity of Estrogen receptor-alpha. Northern Blot analysis detected a ubiquitous expression of MTA2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol MTA2