![Western Blot: MT-ND3 Antibody [NBP2-93832] - Analysis of extracts of various cell lines, using MT-ND3 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/MT-ND3-Antibody-Western-Blot-NBP2-93832-img0007.jpg "Western Blot: MT-ND3 Antibody [NBP2-93832] - Analysis of extracts of various cell lines, using MT-ND3 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1-70 of human MT-ND3 (YP_003024033.1). MNFALILMINTLLALLLMIITFWLPQLNGYMEKSTPYECGFDPMSPARVPFSMKFFLVAITFLLFDLEIA |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ND3 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Theoretical MW | 13 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 50% glycerol |
| Preservative | 0.05% Proclin 300 |
| Purity | Affinity purified |
| Publication using NBP2-93832 | Applications | Species |
|---|---|---|
| Tan L, Qi X, Kong W et al. A conditional knockout rat resource of mitochondrial protein-coding genes via a DdCBE-induced premature stop codon Science advances 2023-04-14 [PMID: 37058569] (WB, KO, Rat) | WB, KO | Rat |
Secondary Antibodies |
Isotype Controls |
Research Areas for MT-ND3 Antibody (NBP2-93832)Find related products by research area.
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| Gene Symbol | ND3 |
![Immunocytochemistry/Immunofluorescence: MT-ND3 Antibody [NBP2-93832] - Analysis of L929 cells using MT-ND3 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/MT-ND3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-93832-img0001.jpg "Immunocytochemistry/Immunofluorescence: MT-ND3 Antibody [NBP2-93832] - Analysis of L929 cells using MT-ND3 at dilution of 1:100. Blue: DAPI for nuclear staining.")
![Immunohistochemistry-Paraffin: MT-ND3 Antibody [NBP2-93832] - Mouse kidney using MT-ND3 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/MT-ND3-Antibody-Immunohistochemistry-Paraffin-NBP2-93832-img0004.jpg "Immunohistochemistry-Paraffin: MT-ND3 Antibody [NBP2-93832] - Mouse kidney using MT-ND3 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.")
![Immunohistochemistry-Paraffin: MT-ND3 Antibody [NBP2-93832] - Human appendix using MT-ND3 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/MT-ND3-Antibody-Immunohistochemistry-Paraffin-NBP2-93832-img0002.jpg "Immunohistochemistry-Paraffin: MT-ND3 Antibody [NBP2-93832] - Human appendix using MT-ND3 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.")
![Immunohistochemistry-Paraffin: MT-ND3 Antibody [NBP2-93832] - Human colon carcinoma using MT-ND3 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/MT-ND3-Antibody-Immunohistochemistry-Paraffin-NBP2-93832-img0003.jpg "Immunohistochemistry-Paraffin: MT-ND3 Antibody [NBP2-93832] - Human colon carcinoma using MT-ND3 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.")
![Immunoprecipitation: MT-ND3 Antibody [NBP2-93832] - Analysis of 200ug extracts of HeLa cells using 3ug MT-ND3 antibody. Western blot was performed from the immunoprecipitate using MT-ND3 antibody at a dilition of 1:500.](http://images.novusbio.com/fullsize/MT-ND3-Antibody-Immunoprecipitation-NBP2-93832-img0006.jpg "Immunoprecipitation: MT-ND3 Antibody [NBP2-93832] - Analysis of 200ug extracts of HeLa cells using 3ug MT-ND3 antibody. Western blot was performed from the immunoprecipitate using MT-ND3 antibody at a dilition of 1:500.")
![Norrin/NDP Antibody [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Norrin/NDP Antibody [Unconjugated]")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")