![Western Blot: MRGPRF Antibody [NBP1-83341] - Analysis in control (vector only transfected HEK293T lysate) and MRGPRF over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/MRGPRF-Antibody-Western-Blot-NBP1-83341-img0010.jpg "Western Blot: MRGPRF Antibody [NBP1-83341] - Analysis in control (vector only transfected HEK293T lysate) and MRGPRF over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: MAGNCSWEAHPGNRNRMCPGLSEAPELYSRGFLTIEQIAMLP |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | MRGPRF |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for MRGPRF Antibody (NBP1-83341)Find related products by research area.
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| Gene Symbol | MRGPRF |
![Immunocytochemistry/Immunofluorescence: MRGPRF Antibody [NBP1-83341] - Staining of human cell line BJ shows localization to nuclear membrane & plasma membrane. Antibody staining shown in green.](http://images.novusbio.com/fullsize/MRGPRF-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83341-img0009.jpg "Immunocytochemistry/Immunofluorescence: MRGPRF Antibody [NBP1-83341] - Staining of human cell line BJ shows localization to nuclear membrane & plasma membrane. Antibody staining shown in green.")
![Immunohistochemistry-Paraffin: MRGPRF Antibody [NBP1-83341] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/MRGPRF-Antibody-Immunohistochemistry-Paraffin-NBP1-83341-img0015.jpg "Immunohistochemistry-Paraffin: MRGPRF Antibody [NBP1-83341] - Staining of human skeletal muscle shows no positivity in myocytes as expected.")
![Immunohistochemistry-Paraffin: MRGPRF Antibody [NBP1-83341] - Staining of human heart muscle shows moderate positivity of the nuclear membrane in myocytes.](http://images.novusbio.com/fullsize/MRGPRF-Antibody-Immunohistochemistry-Paraffin-NBP1-83341-img0006.jpg "Immunohistochemistry-Paraffin: MRGPRF Antibody [NBP1-83341] - Staining of human heart muscle shows moderate positivity of the nuclear membrane in myocytes.")
![Immunohistochemistry-Paraffin: MRGPRF Antibody [NBP1-83341] - Analysis in human prostate and skeletal muscle tissues. Corresponding MRGPRF RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/MRGPRF-Antibody-Immunohistochemistry-Paraffin-NBP1-83341-img0011.jpg "Immunohistochemistry-Paraffin: MRGPRF Antibody [NBP1-83341] - Analysis in human prostate and skeletal muscle tissues. Corresponding MRGPRF RNA-seq data are presented for the same tissues.")
![Immunohistochemistry: MRGPRF Antibody [NBP1-83341] - Staining of human endometrium shows weak to moderate membranous positivity in stromal cells.](http://images.novusbio.com/fullsize/MRGPRF-Antibody-Immunohistochemistry-NBP1-83341-img0012.jpg "Immunohistochemistry: MRGPRF Antibody [NBP1-83341] - Staining of human endometrium shows weak to moderate membranous positivity in stromal cells.")
![Immunohistochemistry-Paraffin: MRGPRF Antibody [NBP1-83341] - Staining of human kidney shows moderate membranous positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/MRGPRF-Antibody-Immunohistochemistry-Paraffin-NBP1-83341-img0013.jpg "Immunohistochemistry-Paraffin: MRGPRF Antibody [NBP1-83341] - Staining of human kidney shows moderate membranous positivity in cells in glomeruli.")
![Immunohistochemistry-Paraffin: MRGPRF Antibody [NBP1-83341] - Staining of human prostate shows moderate membranous positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/MRGPRF-Antibody-Immunohistochemistry-Paraffin-NBP1-83341-img0014.jpg "Immunohistochemistry-Paraffin: MRGPRF Antibody [NBP1-83341] - Staining of human prostate shows moderate membranous positivity in smooth muscle cells.")
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![Immunocytochemistry c-jun [p Ser63] Antibody (1018Y) [Unconjugated]](https://images.novusbio.com/images2/cJun_MAB8930_Immunocytochemistry__Immunofluorescence_18945.jpg)
![Simple Western c-jun [p Ser63] Antibody (1018Y) [Unconjugated]](https://images.novusbio.com/images2/cJun_MAB8930_Simple_Western_19175.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/DATA_IL6_M6000_ELISA_936.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/IL-6_M6000_ELISA_415.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images/m6000b_mouse-il-6-quantikine-elisa-kit-44202415433118.jpg)
![N/A Endostatin [HRP]](https://images.novusbio.com/images2/DATA_Endostatin_DNST0_ELISA_778.jpg)
![N/A Endostatin [HRP]](https://images.novusbio.com/images2/Endostatin_DNST0_ELISA_152.jpg)
![Bioactivity IL-2 [Unconjugated]](https://images.novusbio.com/images/202-il_recombinant-human-il-2-protein-bioactivity-174202314946.jpg)
![SDS-PAGE EGF [Unconjugated]](https://images.novusbio.com/images2/EGF_236-EG_234.jpg)
![Bioactivity EGF [Unconjugated]](https://images.novusbio.com/images2/EGF_236EG_1570.jpg)
![Cell Culture EGF [Unconjugated]](https://images.novusbio.com/images/236-eg_recombinant-human-egf-protein-cf-bioactivity-25202394053.jpg)
![Western Blot Prostatic Acid Phosphatase/ACPP Antibody [Unconjugated]](https://images.novusbio.com/images2/Prostatic_Acid_Phosphatase_AF6240_Western_Blot_10680.jpg)
![Immunohistochemistry Prostatic Acid Phosphatase/ACPP Antibody [Unconjugated]](https://images.novusbio.com/images2/Prostatic_Acid_Phosphatase_AF6240_Immunohistochemistry_10732.jpg)
![ELISA Prostatic Acid Phosphatase/ACPP Antibody [Unconjugated]](https://images.novusbio.com/images2/Prostatic_Acid_Phosphatase_AF6240_ELISA_22233.jpg)
![SDS-PAGE ErbB2/Her2 [Unconjugated]](https://images.novusbio.com/images2/ErbB2_1129-ER_70.jpg)
![Bioactivity ErbB2/Her2 [Unconjugated]](https://images.novusbio.com/images2/1129-er_recombinant-human-erbb2-her2-fc-chimera-protein-cf-bioactivity-7122020142841.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")