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Mouse IL-12 p70 Quantikine ELISA Kit, 2 Plate

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Summary
Reactivity MuSpecies Glossary
Applications ELISA
Conjugate
HRP

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Mouse IL-12 p70 Quantikine ELISA Kit, 2 Plate Summary

Background
The Quantikine Mouse IL-12 p70 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-12 p70 in cell culture supernates and mouse serum. It contains recombinant mouse IL-12 expressed in Sf 21 insect cells using a baculovirus expression vector and antibodies raised against recombinant mouse IL-12. This immunoassay has been shown to accurately quantitate the recombin...ant factor. Results obtained using natural mouse IL-12 showed linear curves that were parallel to the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-12 p70.
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Specificity
Natural and recombinant mouse IL-12 p70
Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
Il12a

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using M1270.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse IL-12 p70 Quantikine ELISA Kit, 2 Plate

  • CLMF p35
  • Cytotoxic lymphocyte maturation factor 35 kDa subunit
  • IL12 p70
  • IL-12 p70
  • IL-12 subunit p35
  • IL-12, subunit p35
  • interleukin 12, p35
  • interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35)
  • interleukin-12 alpha chain
  • interleukin-12 subunit alpha
  • natural killer cell stimulatory factor 1, 35 kD subunit
  • NF cell stimulatory factor chain 1
  • NK cell stimulatory factor chain 1
  • NKSF1
  • p35

Background

Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine that has biological activities similar to, as well as distinct from, IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IL-12 p70/IL-12A (M1270)(119)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Transgenic Mouse.


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Human
(3)
Mouse
(113)
Rat
(4)
Transgenic Mouse
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Showing Publications 1 - 10 of 119. Show All 119 Publications.
Publications using M1270 Applications Species
Blanco, T;Nakagawa, H;Musayeva, A;Krauthammer, M;Singh, RB;Narimatsu, A;Ge, H;Shoushtari, SI;Dana, R; Acquired Immunostimulatory Phenotype of Migratory CD103+ Dendritic Cells Promotes Alloimmunity Following Corneal Transplantation JCI insight 2024-09-05 [PMID: 39235864] (Mouse) Mouse
Bova, V;Basilotta, R;Casili, G;Lanza, M;Filippone, A;Campolo, M;Capra, AP;Vitale, G;Chisari, G;Colarossi, C;Giuffrida, D;Paterniti, I;Esposito, E; The Protective Role of Troxerutin (Trox) in Counteracting Anaplastic Thyroid Carcinoma (ATC) Progression Biomedicines 2024-08-05 [PMID: 39200219] (Human) Human
Roy, S;Roy, S;Halder, S;Jana, K;Ukil, A; Leishmania exploits host cAMP/EPAC/calcineurin signaling to induce an IL-33-mediated anti-inflammatory environment for the establishment of infection The Journal of biological chemistry 2024-05-13 [PMID: 38750790] (Mouse) Mouse
Wang, Z;Chen, Y;Wu, H;Wang, M;Mao, L;Guo, X;Zhu, J;Ye, Z;Luo, X;Yang, X;Liu, X;Yang, J;Sheng, Z;Lee, J;Guo, Z;Liu, Y; Intravenous administration of IL-12 encoding self-replicating RNA-lipid nanoparticle complex leads to safe and effective antitumor responses Scientific reports 2024-03-28 [PMID: 38548896] (Mouse) Mouse
Vannini, A;Parenti, F;Forghieri, C;Vannini, G;Barboni, C;Zaghini, A;Gianni, T;Campadelli-Fiume, G; Decrease in Heparan Sulphate Binding in Tropism-Retargeted Oncolytic Herpes Simplex Virus (ReHV) Delays Blood Clearance and Improves Systemic Anticancer Efficacy Cancers 2024-03-13 [PMID: 38539478] (Mouse) Mouse
Zhang, Y;Song, Y;Wang, X;Shi, M;Lin, Y;Tao, D;Han, S; An NFAT1-C3a-C3aR Positive Feedback Loop in Tumor-Associated Macrophages Promotes a Glioma Stem Cell Malignant Phenotype Cancer immunology research 2024-01-30 [PMID: 38289255] (Mouse) Mouse
Wang, J;Ling, D;Shi, L;Li, H;Peng, M;Wen, H;Liu, T;Liang, R;Lin, Y;Wei, L;Zhang, G;Chen, S; METTL3-mediated m6A methylation regulates ovarian cancer progression by recruiting myeloid-derived suppressor cells Cell & bioscience 2023-11-06 [PMID: 37932814] (Mouse, Transgenic Mouse) Mouse, Transgenic Mouse
You, Y;Kim, SH;Kim, CH;Kim, IH;Shin, Y;Kim, TR;Sohn, M;Park, J; Immune-Stimulating Potential of Lacticaseibacillus rhamnosus LM1019 in RAW 264.7 Cells and Immunosuppressed Mice Induced by Cyclophosphamide Microorganisms 2023-09-13 [PMID: 37764156] (Mouse) Mouse
Jin, J;Fan, YJ;Nguyen, TV;Yu, ZN;Song, CH;Lee, SY;Shin, HS;Chai, OH; Fallopia japonica Root Extract Ameliorates Ovalbumin-Induced Airway Inflammation in a CARAS Mouse Model by Modulating the IL-33/TSLP/NF-?B Signaling Pathway International journal of molecular sciences 2023-08-07 [PMID: 37569890] (Mouse) Mouse
KJ Smith, D Minns, BJ McHugh, RK Holloway, R O'Connor, A Williams, L Melrose, R McPherson, VE Miron, DJ Davidson, E Gwyer Find The antimicrobial peptide cathelicidin drives development of experimental autoimmune encephalomyelitis in mice by affecting Th17 differentiation PloS Biology, 2022-08-26;20(8):e3001554. 2022-08-26 [PMID: 36026478] (Mouse) Mouse
Show All 119 Publications.

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Bioinformatics

Gene Symbol Il12a