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MMP-25/MT6-MMP Antibody (141825) [Unconjugated]

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MMP-25/MT6-MMP was detected in immersion fixed paraffin-embedded sections of human lung using Mouse Anti-Human MMP-25/MT6-MMP Hemopexin Domain Monoclonal Antibody (Catalog # MAB11421) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
MMP-25/MT6-MMP was detected in immersion fixed paraffin-embedded sections of human lung using Mouse Anti-Human MMP-25/MT6-MMP Hemopexin Domain Monoclonal Antibody (Catalog # MAB11421) at 8 µg/mL overnight at 4 °C. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
141825
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB11421-SP
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MMP-25/MT6-MMP Antibody (141825) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human MMP‑25
Pro314-Cys508
Accession # Q9NPA2
Specificity
Detects human MMP‑25/MT6-MMP Hemopexin Domain in Western blots. It recognizes E. coli-expressed hemopexin domain and
NS0‑expressed recombinant human MMP-25.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MMP25
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MMP-25/MT6-MMP Antibody (141825) [Unconjugated]

  • Leukolysin
  • matrix metallopeptidase 25
  • matrix metallopeptidase-like 1
  • matrix metalloproteinase 20
  • matrix metalloproteinase 25
  • matrix metalloproteinase-25
  • matrix metalloproteinase-like 1
  • membrane-type 6 matrix metalloproteinase
  • membrane-type matrix metalloproteinase 6
  • membrane-type-6 matrix metalloproteinase
  • MMP20
  • MMP20A
  • MMP25
  • MMP-25
  • MMPL1
  • MT6MMP
  • MT6-MMP
  • MT-MMP 6
  • MTMMP6
  • MT-MMP6

Background

Matrix Metalloproteinase 25 (MMP-25), also known as MT6-MMP or Leukolysin, is a GPI-linked protease expressed by neutrophils. The extracellular domain of MMP-25 consists of a pro region, a catalytic domain and a hemopexin domain. MMP-25 cleaves collagen type 4, gelatin, fibronectin and fibrin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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MMP-25/MT6-MMP
Antibody (141825) [Un ...
MAB11421
MMP-25/MT6-MMP Antibody (141825) [Unconjugated]
Species: Hu
Applications: WB, IHC

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Bioinformatics

Gene Symbol MMP25
Uniprot