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MMP-16/MT3-MMP Antibody [Unconjugated]

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MMP-16/MT3-MMP was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human MMP-16/MT3-MMP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1785) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
MMP-16/MT3-MMP was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human MMP-16/MT3-MMP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1785) at 15 µg/mL overnight at 4 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
AF1785-SP
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MMP-16/MT3-MMP Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human MMP‑16/MT3‑MMP
Ala32-Gly291 (Ile152Asn)
Accession # P51512
Specificity
Detects human MMP‑16/MT3‑MMP in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MMP16
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF1785 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MMP-16/MT3-MMP Antibody [Unconjugated]

  • chromosome 8 open reading frame 57
  • DKFZp761D112
  • EC 3.4.24
  • EC 3.4.24.-
  • EC 3.4.24.80
  • matrix metallopeptidase 16 (membrane-inserted)
  • matrix metalloproteinase 16 (membrane-inserted)
  • matrix metalloproteinase-16
  • Membrane-type matrix metalloproteinase 3
  • Membrane-type-3 matrix metalloproteinase
  • MMP16
  • MMP-16
  • MMPX2
  • MMP-X2
  • MT3MMP
  • MT3-MMP
  • MT3-MMPC8orf57
  • MT-MMP 3
  • MT-MMP2
  • MTMMP3
  • MT-MMP3
  • Putative transmembrane protein C8orf57

Background

Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix (ECM). MMP-16 (MT3-MMP) is found in brain, lung, placenta, smooth muscle cells, and malignant tumor tissues including oral melanoma and renal carcinoma (1). MMP-16 has been shown to activate proMMP-2 and degrade various ECM components including native collagens (2, 3). MMP-16 has been proposed to possess the potential to directly enhance the growth and invasiveness of cells in vivo, two critical processes for development and carcinogenesis (4). Structurally, MMP-16 consists of the following domains: a pro domain containing the furin cleavage site, a catalytic domain containing the zinc-binding site, a hinge region, a hemopexin-like domain, a transmembrane domain, and a cytoplamasic tail (1). The structure of the catalytic domain in complex with a hydroxamate inhibitor has been solved (5). The rhMMP-16PC consists of the pro and catalytic domains, which can be activated by treatment with furin.

  1. Takino, T. et al. (1995) J. Biol. Chem. 270:23013.
  2. Shofuda, K. et al. (1997) J. Biol. Chem. 272:9749.
  3. Shimada, T. et al. (1999) Eur. J. Biochem. 262:907.
  4. Kang, T. et al. (2000) FASEB J. 14:2559.
  5. Lang, R. et al. (2004) J. Mol. Biol. 336:213.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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MMP-16/MT3-MMP
Antibody [Unconjugate
AF1785
MMP-16/MT3-MMP Antibody [Unconjugated]
Species: Hu
Applications: WB, IHC, IP

Publications for MMP-16/MT3-MMP Antibody (AF1785) (0)

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Review for MMP-16/MT3-MMP Antibody (AF1785) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF1785:
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immunofluorescence - paraffin
(1)
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Human
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Images Ratings Applications Species Date Details
Other MMP-16/MT3-MMP AF1785
Enlarge 		5
reviewed by:
Ruhul Choudhury
 immunofluorescence - paraffin Human 03/18/2017
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Summary

ApplicationOther
Sample TestedFirst trimester decidua
SpeciesHuman

Comments

CommentsSodium citrate antigen retrieval.
Permeabilized with 0.5% triton X.
Primary antibody concentration - 10 ug/ml
Secondary antibody - Rabbit Anti-Goat Alexa Fluor® 568 at 4 ug/ml.


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Other MMP-16/MT3-MMP AF1785
Enlarge
 Ruhul Choudhury
03/18/2017
Application: immunofluorescence - paraffin
Species: Human
read this review

Bioinformatics

Gene Symbol MMP16
Uniprot