![Western Blot: Mitofusin 1 Antibody (11E9-1H12) [NBP1-71775] - Effect of AIF overexpression on mitochondrial fission and fusion in the cortex. Immunoblotting of OPA1 and Mitofusin 1 in the mitochondrial fraction from cortical tissue of the CL and IL hemispheres at 24 h after HI in WT and AIF Tg mice. The expression of Mitofusin 1 was significantly decreased at 24 h post-HI both in WT and AIF Tg mice, while the short form (82 kDa) and cleavage band of OPA1 were significantly increased at 24 h post-HI in both the WT and AIF Tg mice. Quantification of OPA1 and Mitofusin 1 did not show any significant differences in the CL and IL hemispheres between WT and AIF Tg mice (n=6/group). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-020-2280-z), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Mitofusin-1-Antibody-11E9-1H12-Western-Blot-NBP1-71775-img0014.jpg "Western Blot: Mitofusin 1 Antibody (11E9-1H12) [NBP1-71775] - Effect of AIF overexpression on mitochondrial fission and fusion in the cortex. Immunoblotting of OPA1 and Mitofusin 1 in the mitochondrial fraction from cortical tissue of the CL and IL hemispheres at 24 h after HI in WT and AIF Tg mice. The expression of Mitofusin 1 was significantly decreased at 24 h post-HI both in WT and AIF Tg mice, while the short form (82 kDa) and cleavage band of OPA1 were significantly increased at 24 h post-HI in both the WT and AIF Tg mice. Quantification of OPA1 and Mitofusin 1 did not show any significant differences in the CL and IL hemispheres between WT and AIF Tg mice (n=6/group). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-020-2280-z), licensed under a CC-BY license.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC, KO |
| Clone | 11E9-1H12 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Mouse Mitofusin 1 [Swiss-Prot: Q811U4] |
| Localization | Mitochondrion outer membrane; Multi-pass membrane protein |
| Marker | Mitochondrial Fusion Marker |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | MFN1 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | In Western blot, a band can be seen at ~80 kDa. |
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| Theoretical MW | 80 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: Mitofusin 1 Antibody (11E9-1H12) [NBP1-71775] - Antibody was tested in 3T3 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Mitofusin-1-Antibody-11E9-1H12-Immunocytochemistry-Immunofluorescence-NBP1-71775-img0013.jpg "Immunocytochemistry/Immunofluorescence: Mitofusin 1 Antibody (11E9-1H12) [NBP1-71775] - Antibody was tested in 3T3 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).")
![Immunohistochemistry-Paraffin: Mitofusin 1 Antibody (11E9-1H12) [NBP1-71775] - Analysis of Mitofusin-1 in mouse liver.](http://images.novusbio.com/fullsize/Mitofusin-1-Antibody-11E9-1H12-Immunohistochemistry-Paraffin-NBP1-71775-img0011.jpg "Immunohistochemistry-Paraffin: Mitofusin 1 Antibody (11E9-1H12) [NBP1-71775] - Analysis of Mitofusin-1 in mouse liver.")
![Western Blot: Mitofusin 1 Antibody (11E9-1H12) [NBP1-71775] - Analysis of Mitofusin-1 expression in (1) MEF wild-type and (2) MEF Mitofusin-1-null whole cell lysates using NBP1-71775.](http://images.novusbio.com/fullsize/Mitofusin-1-Antibody-11E9-1H12-Western-Blot-NBP1-71775-img0009.jpg "Western Blot: Mitofusin 1 Antibody (11E9-1H12) [NBP1-71775] - Analysis of Mitofusin-1 expression in (1) MEF wild-type and (2) MEF Mitofusin-1-null whole cell lysates using NBP1-71775.")
![Western Blot: Mitofusin 1 Antibody (11E9-1H12) - BSA Free [NBP1-71775] - Effect of AIF overexpression on mitochondrial fission & fusion in the cortex.a Representative immunoblots of mitochondrial fission proteins (P-DRP1 & FIS1) in the mitochondrial fraction from cortical tissue of P9 WT & AIF Tg mice under physiological conditions. Quantification of P-DRP1 & FIS1 did not show a significant difference between WT & AIF Tg mice (n = 6/group). b Representative immunoblots of mitochondrial fusion proteins (OPA1 & MFN1) in the mitochondrial fraction from cortical tissue of P9 WT & AIF Tg mice under physiological conditions. Quantification of OPA1 & MFN1 did not show any significant difference between WT & AIF Tg mice (n = 6/group). c Immunoblotting of P-DRP1and FIS1 in the mitochondrial fraction from the cortical tissue of the CL & IL hemispheres in WT & AIF Tg mice at 24 h after HI. Quantification of P-DRP1 & FIS1 did not show any significant differences in the CL & IL hemispheres between WT & AIF Tg mice (n = 6/group). d Immunoblotting of OPA1 & MFN1 in the mitochondrial fraction from cortical tissue of the CL & IL hemispheres at 24 h after HI in WT & AIF Tg mice. The expression of MFN1 was significantly decreased at 24 h post-HI both in WT & AIF Tg mice, while the short form (82 kDa) & cleavage band of OPA1 were significantly increased at 24 h post-HI in both the WT & AIF Tg mice. Quantification of OPA1 & MFN1 did not show any significant differences in the CL & IL hemispheres between WT & AIF Tg mice (n = 6/group). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32001673), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-71775_mouse-monoclonal-mitofusin-1-antibody-11e9-1h12-310202416163735.jpg "Western Blot: Mitofusin 1 Antibody (11E9-1H12) - BSA Free [NBP1-71775] - Effect of AIF overexpression on mitochondrial fission & fusion in the cortex.a Representative immunoblots of mitochondrial fission proteins (P-DRP1 & FIS1) in the mitochondrial fraction from cortical tissue of P9 WT & AIF Tg mice under physiological conditions. Quantification of P-DRP1 & FIS1 did not show a significant difference between WT & AIF Tg mice (n = 6/group). b Representative immunoblots of mitochondrial fusion proteins (OPA1 & MFN1) in the mitochondrial fraction from cortical tissue of P9 WT & AIF Tg mice under physiological conditions. Quantification of OPA1 & MFN1 did not show any significant difference between WT & AIF Tg mice (n = 6/group). c Immunoblotting of P-DRP1and FIS1 in the mitochondrial fraction from the cortical tissue of the CL & IL hemispheres in WT & AIF Tg mice at 24 h after HI. Quantification of P-DRP1 & FIS1 did not show any significant differences in the CL & IL hemispheres between WT & AIF Tg mice (n = 6/group). d Immunoblotting of OPA1 & MFN1 in the mitochondrial fraction from cortical tissue of the CL & IL hemispheres at 24 h after HI in WT & AIF Tg mice. The expression of MFN1 was significantly decreased at 24 h post-HI both in WT & AIF Tg mice, while the short form (82 kDa) & cleavage band of OPA1 were significantly increased at 24 h post-HI in both the WT & AIF Tg mice. Quantification of OPA1 & MFN1 did not show any significant differences in the CL & IL hemispheres between WT & AIF Tg mice (n = 6/group). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32001673), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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